Use of the yeast two-hybrid system to identify targets of fungal effectors

Methods in Molecular Biology

Volumn 835, Issue , 2012, Pages 165-189

  Lu, Shunwen ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

Galactosidase assay; Aureobasidin A; Autoactivation; Fungal pathogens; Plant disease; Protein protein interactions; Reporter genes; Synthetic dropout media; Yeast transformation

Indexed keywords

DNA BINDING PROTEIN; FUNGAL PROTEIN; GAL4 PROTEIN, S CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEIN; TRANSCRIPTION FACTOR; VEGETABLE PROTEIN;

ARTICLE; FUNGUS; GENE LIBRARY; HOST PATHOGEN INTERACTION; METABOLISM; MICROBIOLOGY; PHYSIOLOGY; PLANT; REPORTER GENE; SACCHAROMYCES CEREVISIAE; TWO HYBRID SYSTEM;

DNA-BINDING PROTEINS; FUNGAL PROTEINS; FUNGI; GENE LIBRARY; GENES, REPORTER; HOST-PATHOGEN INTERACTIONS; PLANT PROTEINS; PLANTS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TRANSCRIPTION FACTORS; TWO-HYBRID SYSTEM TECHNIQUES;

ARABIDOPSIS;

EID: 84855883832     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-501-5_11     Document Type: Article

References (37)

1. Fields S., and Song O. (1989). A novel genetic system to detect protein-protein interactions. Nature 340, 245-246. (Pubitemid 19171591)
2. Chien C.T., Bartel P.L., Sternglanz R., and Fields S. (1991). The two-hybrid system: A method to identify and clone genes for proteins that interact with a protein of interest. Proc Natl Acad Sci USA 88, 9578-9582. (Pubitemid 21915674)
3. Fields S., and Sternglanz R. (1994). The twohybrid system: An assay for protein-protein interactions. Trends Genet 10, 286-292. (Pubitemid 24238900)
4. Van Criekinge W., and Beyaert R. (1999). Yeast Two-Hybrid: State of the art. Biol Proced Online 2, 1-38.
5. Walhout A.J., Boulton S.J., and Vidal M. (2000). Yeast two-hybrid systems and protein interaction mapping projects for yeast and worm. Yeast 17, 88-94. (Pubitemid 30466087)
6. Fields S., and Bartel P.L. (2001). The two-hybrid system. A personal view. Methods Mol Biol 177, 3-8.
7. Causier B., and Davies B. (2002). Analysing protein-protein interactions with the yeast twohybrid system. Plant Mol Biol 50, 855-870. (Pubitemid 36028898)
8. Parrish J.R., Gulyas K.D., and Finley R.L., Jr. (2006). Yeast two-hybrid contributions to interactome mapping. Curr Opin Biotechnol 17, 387-393. (Pubitemid 44163455)
9. Yu, Haiyuan, et al (2008). High-quality binary protein interaction map of the yeast interactome network. Science 322, 104-110.
10. Bruckner A., Polge, C., Lentze N., Auerbach D., and Schlattner U. (2009). Yeast two-hybrid, a powerful tool for systems biology. Int J Mol Sci 10, 2763-2788.
11. De Las Rivas J., and Fontanillo C. (2010). Protein-protein interactions essentials: Key concepts to building and analyzing interactome networks. PLoS Comput Biol 6, e1000807.
12. Johnston S.A., Salmeron J.M., Jr., and Dincher S.S. (1987). Interaction of positive and negative regulatory proteins in the galactose regulon of yeast. Cell 50, 143-146.
13. Deane C.M., Salwinski L., Xenarios I., and Eisenberg D. (2002). Protein interactions: Two methods for assessment of the reliability of high throughput observations. Mol Cell Proteomics 1, 349-356.
14. Manning V.A., Hardison L.K., and Ciuffetti L.M. (2007). Ptr ToxA interacts with a chloroplast-localized protein. Mol Plant Microbe Interact 20, 168-177. (Pubitemid 46146180)
15. Tai Y., Bragg J., and Meinhardt S. (2007). Functional characterization of ToxA and molecular identifi cation of its intracellular targeting protein in wheat American J. Plant Physiol. 2, 76-89. (Pubitemid 350286935)
16. Wolpert T.J., Dunkle L.D., and Ciuffetti L.M. (2002). Host-selective toxins and avirulence determinants: What's in a name? Annu Rev Phytopathol 40, 251-285. (Pubitemid 35235582)
17. Friesen T.L., Meinhardt S.W., and Faris J.D. (2007) The Stagonospora nodorum -wheat pathosystem involves multiple proteinaceous host-selective toxins and corresponding host sensitivity genes that interact in an inverse genefor-gene manner. Plant J 51 , 681-92. (Pubitemid 47231188)
18. Stergiopoulos I., and de Wit P.J. (2009). Fungal effector proteins. Annu Rev Phytopathol 47, 233-263.
19. Ballance G.M., Lamari L., Kowatsch R., and Bernier C.C. (1996). Cloning, expression and occurrence of a gene encoding the Ptr necrosis toxin from Pyrenophora tritici-repentis Mol. Plant Path . On-Line ( http://www.bspp.org.uk/ mppol/ ) 1996/1209ballance).
20. Ciuffetti L.M., Tuori R.P., and Gaventa J.M. (1997). A single gene encodes a selective toxin causal to the development of tan spot of wheat. Plant Cell 9, 135-144. (Pubitemid 27161888)
21. Friesen T.L., Stukenbrock E.H., Liu Z., Meinhardt S., Ling H., Faris J.D., Rasmussen J.B., Solomon P.S., McDonald B.A., and Oliver R.P. (2006). Emergence of a new disease as a result of interspecifi c virulence gene transfer. Nat Genet 38, 953-956. (Pubitemid 44141666)
22. Faris, J.D. Zhang, Z. Lu H., Lu S., Reddy L., Cloutier S., Fellers J.P., Meinhardt S.W., Rasmussen J.B., Xu S.S., Oliver R.P., Simons K.J., and Friesen T.L. (2010). A unique wheat disease resistance-like gene governs effectortriggered susceptibility to necrotrophic pathogens. Proc Natl Acad Sci USA 107, 13544-13549.
23. Zhu Y.Y., Machleder E.M., Chenchik A., Li, R., and Siebert P.D. (2001). Reverse transcriptase template switching: a SMART approach for fulllength cDNA library construction. Biotechniques 30, 892-897. (Pubitemid 32319835)
24. Shcheglov A., Zhulidov P, Bogdanova E. and Shagin D. (2007) Generation of normalized cDNA libraries. In Nucleic acids hybridization modern applications. Buzdin A. and Lukyanov S. Eds. Springer, Dordrecht, The Netherlands. pp. 316.
25. Jacobs J.L., Belew A.T., Rakauskaite R. and Dinman J.D. (2007) Identifi cation of functional, endogenous programmed-1 ribosomal frameshift signals in the genome of Saccharomyces cerevisiae . Nucleic Acids Res 35, 165-174. (Pubitemid 46189841)
26. Naumovski L. (2001). Two-hybrid interactions confi rmed by coimmunoprecipitation of epitope-tagged clones. Methods Mol Biol 177, 151-159.
27. Lee C. (2007). Coimmunoprecipitation assay. Methods Mol Biol 362, 401-406. (Pubitemid 350183185)
28. Kraichely D.M., and MacDonald P.N. (2001). Confi rming yeast two-hybrid protein interactions using in vitro glutathione-S-transferase pulldowns. Methods Mol Biol 177, 135-150.
29. Vikis H.G., and Guan K.L. (2004). Glutathione- S-transferase-fusion based assays for studying protein-protein interactions. Methods Mol Biol 261, 175-186.
30. Garcia-Cuellar M.P., Mederer D., and Slany R.K. (2009). Identifi cation of protein interaction partners by the yeast two-hybrid system. Methods Mol Biol 538, 347-367.
31. Barnard E., McFerran N.V., Trudgett A., Nelson J., and Timson D.J. (2008). Development and implementation of split-GFP-based bimolecular fluorescence complementation (BiFC) assays in yeast. Biochem Soc Trans 36, 479-482. (Pubitemid 351839359)
32. Arndt-Jovin D.J., and Jovin T.M. (1989). Fluorescence labeling and microscopy of DNA. Methods Cell Biol 30, 417-448.
33. Sekar R.B., and Periasamy A. (2003). Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations. J Cell Biol 160, 629-633. (Pubitemid 36298259)
34. Takesako K., Kuroda H., Inoue T., Haruna F., Yoshikawa Y., Kato I., Uchida K., Hiratani T., and Yamaguchi H. (1993). Biological properties of aureobasidin A, a cyclic depsipeptide antifungal antibiotic. J Antibiot (Tokyo) 46, 1414-1420. (Pubitemid 23290990)
35. Hashida-Okado T., Ogawa A., Endo M., Yasumoto R., Takesako K., and Kato I. (1996). AUR1 , a novel gene conferring aureobasidin resistance on Saccharomyces cerevisiae : a study of defective morphologies in Aur1p-depleted cells. Mol Gen Genet 251, 236-244.
36. Guo D., Rajamaki M.L., and Valkonen J. (2008). Protein-protein interactions: The yeast two-hybrid system . Methods Mol Biol 451, 421-439.
37. Iyer K., Burkle L., Auerbach D., Thaminy S., Dinkel M., Engels K., and Stagljar I. (2005). Utilizing the split-ubiquitin membrane yeast two-hybrid system to identify protein-protein interactions of integral membrane proteins. Sci STKE 2005, p. l3.