Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations

Journal of Cell Biology

Volumn 160, Issue 5, 2003, Pages 629-633

  Sekar, Rajesh Babu ^a   Periasamy, Ammasi ^a  

^a UNIVERSITY OF VIRGINIA   (United States)

Author keywords

Cameleons; Data analysis; Dimerization; FRET assays; FRET microscopy

Indexed keywords

CALCIUM ION; COMPLEMENTARY DNA; CYCLIC AMP; FLUORESCENT DYE; GREEN FLUORESCENT PROTEIN; INTEGRIN; MYOSIN LIGHT CHAIN KINASE; NUCLEIC ACID; PROTEIN; RAC PROTEIN; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN; RHODAMINE;

CELLULAR DISTRIBUTION; CHROMATOPHORE; DATA ANALYSIS; FLUORESCENCE MICROSCOPY; FLUORESCENCE RESONANCE ENERGY TRANSFER MICROSCOPY; GENE EXPRESSION; HUMAN; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTEIN LOCALIZATION; SHORT SURVEY;

ANIMALS; EUKARYOTIC CELLS; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; GREEN FLUORESCENT PROTEINS; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; PROTEINS;

EID: 0037416207     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.200210140     Document Type: Short Survey

References (28)

1. Adams, S.R., A.T. Harootunian, Y.J. Buechler, S.S. Taylor, and R.Y. Tsien. 1991. Fluorescence ratio imaging of cyclic AMP in single cells. Nature. 349:694-697.
2. Bastiaens, P.I., and A. Squire. 1999. Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell. Trends Cell Biol. 9:48-52.
3. Chen, X., B. Zehnbauer, A. Gnirke, and P.Y. Kwok. 1997. Fluorescence energy transfer detection as a homogeneous DNA diagnostic method. Proc. Natl. Acad. Sci. USA. 94:10756-10761.
4. Clegg, R.M. 1996. Fluorescence resonance energy transfer. In Fluorescence Imaging Spectroscopy and Microscopy. Vol. 137. X.F. Wang and B. Herman, editors. John Wiley & Sons Inc., New York. 179-251.
5. Cole, N.B., C.L. Smith, N. Sciaky, M. Terasaki, M. Edidin, and J.L. Schwartz. 1996. Diffusional mobility of Golgi proteins in membranes of living cells. Science. 273:797-801.
6. Day, R.N. 1998. Visualization of Pit-1 transcription factor interactions in the living cell nucleus by fluorescence resonance energy transfer microscopy. Mol. Endocrinol. 12:1410-1419.
7. dos Remedios, C.G., M. Miki, and J.A. Barden. 1987. Fluorescence resonance energy transfer measurements of distances in actin and myosin: A critical evaluation. J. Muscle Res. Cell Motil. 8:97-117.
8. Elangovan, M., R.N. Day, and A. Periasamy. 2002. Nanosecond fluorescence resonance energy transfer-fluorescence lifetime imaging microscopy to localize the protein interactions in a single living cell. J. Microsc. 205:3-14.
9. Elangovan, M., H. Wallrabe, Y. Chen, R.N. Day, M. Barroso, and A. Periasamy. 2003. Characterization of one- and two-photon excitation fluorescence resonance energy transfer microscopy. Methods. 29:58-73.
10. Förster, T. 1965. Delocalized excitation and excitation transfer. In Modern Quantum Chemistry. Vol. 3. O. Sinanoglu, editor. Academic Press Inc., New York. 93-137.
11. Gadella, T.W.J., Jr., and T.M. Jovin. 1995. Oligomerization of epidermal growth factor receptors on A431 cells studied by time-resolved fluorescence imaging microscopy. A stereochemical model for tyrosine kinase receptor activation. J. Cell Biol. 129:1543-1548.
12. Giordano, L., T.M. Jovin, M. Irie, and E.A. Jares-Erijman. 2002. Diheteroarylethenes as thermally stable photoswitchable acceptors in phorochromic fluorescence resonance energy transfer (pcFRET). J. Am. Chem. Soc. 124:7481-7489.
13. Gordon, G.W., G. Berry, X.H. Liang, B. Levine, and B. Herman. 1998. Quantitative fluorescence resonance energy transfer measurements using fluorescence microscopy. Biophys. J. 74:2702-2713.
14. Hanson, M.R., and R.H. Kohler. 2001. GFP imaging: methodology and application to investigate cellular comparrmentation in plants. J. Exp. Bot. 52:529-539.
15. Heim, R., and R.Y. Tsien. 1996. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr. Biol. 6:178-182.
16. Herman, B. 1998. Fluorescence Microscopy. 2nd ed. Springer-Verlag New York Inc., New York. 170 pp.
17. Kraynov, V.S., C. Chamberlain, G.M. Bokoch, M.A. Schwartz, S. Slabaugh, and K.M. Hahn. 2000. Localized Rac activation dynamics visualized in living cells. Science. 290:333-337.
18. Lakowicz, J.R. 1999. Principles of Fluorescence Spectroscopy. 2nd ed. Plenum Publishing Corp., New York. 692 pp.
19. 2+ based on green fluorescent proteins and calmodulin. Nature. 388:882-887.
20. Periasamy, A. 2001. Methods in Cellular Imaging. Oxford University Press, New York. 434 pp.
21. Pollok, B.A., and R. Heim. 1999. Using GFP in FRET-based applications. Trends Cell Biol. 9:57-60.
22. Pozo, M.A.D., W.B. Kiosses, N.B. Alderson, N. Meller, K.M. Hahn, and M.A. Schwartz. 2002. Integrins regulate GTP-Rac localized effector interactions through dissociation of Rho-GDI. Nat. Cell Biol. 4:232-239.
23. Roessel, P.V., and A.H. Brand. 2002. Imaging into the future: visualizing gene expression and protein interactions with fluorescent proteins. Nat. Cell Biol. 4:E15-E20.
24. Struck, D.K., D. Hoekstra, and R.E. Pagano. 1981. Use of resonance energy transfer to monitor membrane fusion. Biochemistry. 20:4093-4099.
25. Truong, K., and M. Ikura. 2001. The use of FRET imaging microscopy to detect protein-protein interactions and protein conformational changes in vivo. Curr. Opin. Struct. Biol. 11:573-578.
26. 2+ imaging by a new calmodulin-GFP fusion molecule. Nat. Strucr. Biol. 8:1069-1073.
27. Ting, A.Y., K.H. Kain, R.L. Klemke, and R.Y. Tsien. 2001. Genetically encoded fluorescent reporters of protein tyrosine kinase activities in living cells. Proc. Natl. Acad. Sci. USA. 98:15003-15008.
28. Xia, Z., and Y. Liu. 2001. Reliable and global measurement of fluorescence resonance energy transfer using fluorescence microscopes. Biophys. J. 81:2395-2402.