Antifibrotic and antioxidant effects of N-acetylcysteine in an experimental cholestatic model

European Journal of Gastroenterology and Hepatology

Volumn 24, Issue 2, 2012, Pages 179-185

  Galicia Moreno, Marina ^a   Favari, Liliana ^a   Muriel, Pablo ^a  

^a DEPARTAMENTO DE FÍSICA   (Mexico)

Author keywords

biliary cirrhosis; cytokines; fibrosis; lipid peroxidation; N acetylcysteine; oxidative stress

Indexed keywords

ACETYLCYSTEINE; ALANINE AMINOTRANSFERASE; ANTIFIBROTIC AGENT; CATALASE; GLUTATHIONE; HYDROXYPROLINE; INTERLEUKIN 10; INTERLEUKIN 6; LIPID; TRANSFORMING GROWTH FACTOR BETA;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIOXIDANT ACTIVITY; ARTICLE; BILE DUCT LIGATION; BILIARY CIRRHOSIS; CHOLESTASIS; CONTROLLED STUDY; DENSITOMETRY; DRUG EFFICACY; IMMUNOMODULATION; LIPID PEROXIDATION; LIVER FIBROSIS; LIVER INJURY; MALE; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; WESTERN BLOTTING; WISTAR RAT;

ACETYLCYSTEINE; ALANINE TRANSAMINASE; ANIMALS; ANTIOXIDANTS; CATALASE; CHOLESTASIS; CYTOKINES; DISEASE MODELS, ANIMAL; DRUG EVALUATION, PRECLINICAL; FIBROSIS; GLUTATHIONE; INFLAMMATION MEDIATORS; LIPID PEROXIDATION; LIVER; MALE; OXIDATIVE STRESS; RATS; RATS, WISTAR;

EID: 84855869948     PISSN: 0954691X     EISSN: 14735687     Source Type: Journal    
DOI: 10.1097/MEG.0b013e32834f3123     Document Type: Article

References (47)

1. Muriel P. Some experimental models of liver damage in hepatotoxicity: from genomics to in vitro and in vivo models.John Wiley & Sons; 2007. pp. 119-137.
2. Schaffner F, Popper H. Classification and mechanism of cholestasis in Liver and biliary disease Pathophysiology Diagnosis Management.WB Saunders Company Ltd; 1979. pp. 296-323.
3. Sherlock S. Cholestasis in diseases of the liver and biliary system. Blackwell Scientific Publications; 1989. pp. 248-272.
4. Li MK, Crawford JM. The pathology of cholestasis. Semin Liver Dis 2004; 24:21-24.
5. Poli G. Pathogenesis of liver fibrosis: role of oxidative stress. Mol Aspects Med 2000; 21:49-98. (Pubitemid 30642764)
6. Tahan G, Tarcin O, Tahan V, Eren F, Gedik N, Sahan E, et al. The effects of N-acetylcysteine on bile-duct ligation-induced liver fibrosis in rats. Dig Dis Sci 2007; 52:3348-3354. (Pubitemid 350077177)
7. Wang G, Shen H, Rajaraman G, Roberts MS, Gong Y, Jiang P, et al. Expression and antioxidant function of liver fatty acid binding protein in normal and bile-duct ligated rats. Eur J Pharmacol 2007; 560: 61-68.
8. Orellana M, Avalos N, Abarca M, Valdé s E. Microsomal and peroxisomal fatty acid oxidation in bile duct ligated rats: A comparative study between liver and kidney. Gen Pharmacol 1997; 28:525-529. (Pubitemid 27156914)
9. Portincasa P, Grattagliano I, Testini M, Caruso ML,Wang DQ, Moschetta A, et al. Parallel intestinal and liver injury during early cholestasis in the rat: Modulation by bile salts and antioxidants. Free Radic Biol Med 2007; 42:1381-1391. (Pubitemid 46476849)
10. Assimakopoulos SF, Maroulis I, Patsoukis N, Vagenas K, Scopa CD, Georgiou CD, et al. Effect of antioxidant treatments on the gut-liver axis oxidative status and function in bile duct-ligated rats. World J Surg 2007; 31:2023-2032. (Pubitemid 47415730)
11. Svegilati G, D'Ambrosio L, Ferreti G, Casini A, Di Sario A, Salzano R, et al. Fibrogenic effect of oxidative stress on rat hepatic stellate cells. Hepatology 1998; 27:720-726. (Pubitemid 28103548)
12. Liu RM, Gaston Pravia KA. Oxidative stress and glutathione in TGF-b mediated fibrogenesis. Free Radic Biol Med 2010; 48:1-15.
13. Houglum K, Lee KS, Chojkier M. Proliferation of hepatic stellate cells is inhibited by phosphorylation of CREB on serine 133. J Clin Invest 1997; 99:1322-1328. (Pubitemid 27142770)
14. Kawada N, Seki S, Inoue M, Kuroki T. Effect of antioxidants resveratrol, quercetin, and N-acetylcysteine, on the functions of cultured rat hepatic stellate cells and Kupffer cells. Hepatology 1998; 27:1265-1274. (Pubitemid 28227853)
15. De Vries N, De Flora S. N-Acetyl-L-Cysteine. J Cell Biochem Suppl 1993; 17F:270-277. (Pubitemid 23252953)
16. Kobrinsky NL, Sjolander DE, Goldenberg JA, Ortmeier TC. Successful treatment of doxorubicin and cisplatin resistant hepatoblastoma in a child with Beckwith-Wiedemann syndrome with high dose acetaminophen and N-acetylcysteine rescue. Pediatr Blood Cancer 2005; 45:222-225. (Pubitemid 40993592)
17. Rodrigues AJ, Evora PR, Schaff HV. Protective effect of N-acetylcysteine against oxygen radical-mediated coronary artery injury. Braz J Med Biol Res 2004; 37:1215-1224. (Pubitemid 39172556)
18. Akgun E, Caliskan C, Celik HA, Ozutemiz AO, Tuncyurek M, Aydin HH. Effects of N-acetylcysteine treatment on oxidative stress in acetic acidinduced experimental colitis in rats. J Int Med Res 2005; 33:196-206. (Pubitemid 40372435)
19. Yagci G, Gul H, Simsek A, Buyukdogan V, Onguru O, Zeybek N, et al. Beneficial effects of N-acetylcysteine on sodium taurocholate-induced pancreatitis in rats. J. Gastroenterol. 2004; 39:268-276. (Pubitemid 38519433)
20. Pereira-Filho G, Ferreira C, Schwengber A, Marroni C, Zettler C, Marroni N. Role of N-acetylcysteine on fibrosis and oxidative stress in cirrhotic rats. Arq Gastroenterol 2008; 45:156-162.
21. Vendemiale G, Grattagliano I, Caruso ML, Serviddio G, Valentini AM, Pirreli M, et al. Increased oxidative stress in dimethylnitrosamine induced liver fibrosis in the rat: Effect of N-acetylcysteine and interferon-alpha. Toxicol Appl Pharmacol 2001; 175:130-139. (Pubitemid 32848504)
22. Reitman S, Frankel S. A colorimetric method for determination of serum oxaloacetic and glutamic pyruvic transaminases. Am J Clin Pathol 1957; 28:56-63.
23. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by the thiobarbituric acid reaction. Anal Biochem 1979; 95:351-358. (Pubitemid 9194347)
24. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 1976; 72:248-254.
25. Hissin PJ, Hilf R. A florometric method for determination of oxidized and reduced glutathione in tissues. Anal Biochem 1976; 74:214-226.
26. Cohen G, Dembiec D, Marcus J. Measurement of catalase activity in tissue extracts. Anal Biochem 1970; 34:30-38.
27. Prockop DJ, Udenfriend S. A specific method for the analysis of hydroxyproline in tissues and urine. Anal Biochem 1960; 1:228-239.
28. Muriel P, Deheza R. Fibrosis and glycogen stores depletion induced by prolonged biliary obstruction in the rat are ameliorate by metadoxine. Liver Int 2003; 23:262-268. (Pubitemid 37265802)
29. García Tovar CG, Perez A, Luna J, Mena R, Osorio B, Aleman V, et al. Biochemical and histochemical analysis of 71 kDa dystrophin isoform (Dp71f) in rat brain. Acta Histochem 2001; 103:209-224. (Pubitemid 32385679)
30. Parola M, Robino G. Oxidative stress-related molecules and liver fibrosis. J Hepatol 2001; 35:297-306. (Pubitemid 32803489)
31. Harrison PM, Wendon JA, Gimson AE, Alexander GJ, Williams R. Improvement by acetylcysteine of hemodynamics and oxygen transport in fulminant hepatic failure. N Engl J Med 1991; 324:1852-1857.
32. Wu G, Fang YZ, Yang S, Lupton JR, Turner ND. Glutathione metabolism and its implications for health. J Nutr 2004; 134:489-492. (Pubitemid 38298485)
33. Friedman SL. Cytokines and fibrogenesis. Semin Liver Dis 1999; 19:129-140. (Pubitemid 29326120)
34. Millea PJ. N-acetylcysteine: Multiple clinical applications. Am Fam Physician 2009; 80:265-269.
35. Blum J, Fridovich I. Inactivation of glutathione peroxidase by superoxide radical. Arch Biochem Biophys 1985; 240:500-508. (Pubitemid 15250062)
36. Pastor A, Collado PS, Almar M, Gonzáles-Gallego J. Antioxidant enzyme status in biliary obstructed rats: Effects of N-acetylcysteine. J Hepatol 1997; 27:363-370. (Pubitemid 27366218)
37. De Bleser P, Xu G, Rombouts K, Rogiers V, Geerts A. Glutathione levels discriminate between oxidative stress and transforming growth factor-b signaling in activated rat hepatic stellate cells. J Biol Chem 1999; 274:33881-33887. (Pubitemid 129511715)
38. Garcia-Trevijano ER, Iraburu MJ, Fontana L, Domínguez-Rosales JA, Auster A, Covarrubias-Pinedo A, et al. Transforming growth factor beta1 induces the expression of alpha1(I) procollagen mRNA by a hydrogen peroxide-C/ EBPbeta-dependent mechanism in rat hepatic stellate cells. Hepatology 1999; 29:960-970. (Pubitemid 29109616)
39. Herrera B, Murillo MM, Alvarez-Barrientos A, Beltrán J, Fernández M, Fabregat I. Source of early reactive oxygen species in the apoptosis induced by transforming growth factor-beta in fetal hepatocytes. Free Radic Biol Med 2004; 36:16-26. (Pubitemid 38068288)
40. Albright CD, Salganik RI, Craciunescu CN, Mar MH, Zeisel SH. Mitochondrial and microsomal derived reactive oxygen species mediate apoptosis induced by transforming growth factor-beta 1 in immortalized rat hepatocytes. J Cell Biochem 2003; 89:254-261 (Pubitemid 36547260)
41. Barcellos-Hoff M, Dix TA. Redox-mediated activation of latent transforming growth factor-beta 1. Mol Endocrinol 1996; 10:1077-1083. (Pubitemid 26299216)
42. Pociask DA, Sime P, Brody AR. Asbestos-derived reactive oxygen species activate TGF-beta1. Lab Invest 2004; 84:1013-1023. (Pubitemid 39025036)
43. Jobling MF, Mott JD, Finnegan MT, Jurukovski V, Erickson AC,Walian PJ, et al. Isoform-specific activation of latent transforming growth factor beta (LTGF-beta) by reactive oxygen species. Radiat Res 2006; 166:839-848. (Pubitemid 44888249)
44. Sullivan DE, Ferris M, Pociask D, Brody AR. The latent form of TGFbeta (1) is induced by TNFalpha through an ERK specific pathway and is activated by asbestos-derived reactive oxygen species in vitro and in vivo. J Immunotoxicol 2008; 5:145-149. (Pubitemid 351882416)
45. Marucci L, Baroni GS, Mancini R, Benedetti A, Jezequel AM, Orlandi F. Cell proliferation following extrahepatic biliary obstruction. Evaluation by immunohistochemical methods. J Hepatol 1993; 17:163-169. (Pubitemid 23054481)
46. Martínez OM, Villanueva JC, Greshwin ME, Krams SM. Cytokine patterns and cytotoxic mediators in primary biliary cirrhosis. Hepatology 1995; 21:113-119.
47. Ramudo L, Manso MA, Vicente S, De Dios I. Pro-and anti-inflammatory response of acinar cells during acute pancreatitis. Effect of N-acetylcysteine. Cytokine 2005; 32:125-131. (Pubitemid 41681784)