Production and purification of the phosphoprotein of Nipah Virus in Escherichia coli for use in diagnostic assays

Biotechnology and Bioprocess Engineering

Volumn 16, Issue 6, 2011, Pages 1166-1172

  Salvamani, Shamala ^a   Tey, Beng Ti ^a,b   Ng, Wen Cheng ^a   Tan, Wen Siang ^a  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

^b MONASH UNIVERSITY MALAYSIA   (Malaysia)

Author keywords

Antigenicity; Diagnostic agent; ELISA; IMAC; Nipah Virus; Phosphoprotein

Indexed keywords

AFFINITY CHROMATOGRAPHY; BINARY ALLOYS; CLONING; ELECTROPHORESIS; ESCHERICHIA COLI; PURIFICATION; RECOMBINANT PROTEINS; VIRUSES;

ANTIGENICITY; DIAGNOSTIC AGENTS; ELISA; IMAC; NIPAH VIRUS; PHOSPHOPROTEIN;

BIOSYNTHESIS;

BACTERIUM LYSATE; IMIDAZOLE; PHOSPHOPROTEIN; POLYHISTIDINE TAG; RECOMBINANT PROTEIN;

ANTIGENICITY; ARTICLE; BACTERIAL CELL; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; DIAGNOSTIC TEST; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FERMENTATION OPTIMIZATION; IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY; MOLECULAR CLONING; NIPAH VIRUS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; SERODIAGNOSIS; TEMPERATURE DEPENDENCE; VIRUS GENE; WESTERN BLOTTING;

ANIMALIA; ESCHERICHIA COLI; NIPAH VIRUS; PARAMYXOVIRIDAE; SUIDAE;

EID: 84855726512     PISSN: 12268372     EISSN: 19763816     Source Type: Journal    
DOI: 10.1007/s12257-011-0095-6     Document Type: Article

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