SYBR Green-based Real-Time PCR targeting kinetoplast DNA can be used to discriminate between the main etiologic agents of Brazilian cutaneous and visceral leishmaniases

Parasites and Vectors

Volumn 5, Issue 1, 2012, Pages

  Pita Pereira, Daniela ^a   Lins, Rachel ^a   Oliveira, Marcia P ^a   Lima, Rosimar B ^a   Pereira, Bernardo A S ^a   Moreira, Otacilio C ^a   Brazil, Reginaldo P ^a   Britto, Constança ^a  

^a INSTITUTO OSWALDO CRUZ   (Brazil)

Author keywords

Brazil; kinetoplast DNA; Leishmaniases; molecular diagnosis; SYBR Green Real time PCR; thermal dissociation curves

Indexed keywords

CYTOSINE; GUANINE; KINETOPLAST DNA;

AMPLICON; ARTICLE; BRAZIL; CONTROLLED STUDY; GENE AMPLIFICATION; LEISHMANIA; LEISHMANIA BRAZILIENSIS; LEISHMANIA INFANTUM; LEISHMANIA SHAWII; LUTZOMYIA; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; SKIN LEISHMANIASIS; SPECIES DIFFERENTIATION; TEMPERATURE; VISCERAL LEISHMANIASIS;

ADOLESCENT; ANIMALS; BASE SEQUENCE; BRAZIL; CHILD; CHILD, PRESCHOOL; CONSERVED SEQUENCE; DNA, KINETOPLAST; FEMALE; FLUORESCENT DYES; HUMANS; INSECT VECTORS; LEISHMANIA; LEISHMANIASIS, CUTANEOUS; LEISHMANIASIS, VISCERAL; MALE; MOLECULAR SEQUENCE DATA; ORGANIC CHEMICALS; PHYLOGENY; PSYCHODIDAE; REAL-TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA;

LEISHMANIA AMAZONENSIS; LEISHMANIA BRAZILIENSIS; LEISHMANIA INFANTUM; VIANNIA;

EID: 84855612110     PISSN: None     EISSN: 17563305     Source Type: Journal    
DOI: 10.1186/1756-3305-5-15     Document Type: Article

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