Advantages and limits of real-time PCR assay and PCR-restriction fragment length polymorphism for the identification of cutaneous Leishmania species in Tunisia

Transactions of the Royal Society of Tropical Medicine and Hygiene

Volumn 105, Issue 1, 2011, Pages 17-22

  Ben Abda, Imène ^a,b   De Monbrison, Frédérique ^b   Bousslimi, Nadia ^a   Aoun, Karim ^a   Bouratbine, Aïda ^a   Picot, Stéphane ^b  

^a PASTEUR INSTITUTE OF TUNIS   (Tunisia)

^b HOSPICES CIVILS DE LYON   (France)

Author keywords

Cutaneous leishmania species; ITS1 PCR RFLP; KDNA Real time PCR; Tunisia

Indexed keywords

ARTICLE; CLINICAL ARTICLE; DIAGNOSTIC ACCURACY; DNA HYBRIDIZATION; DNA SEQUENCE; HUMAN; HUMAN TISSUE; INTERMETHOD COMPARISON; LEISHMANIA; MICROSCOPY; NONHUMAN; PARASITE; REAL TIME POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SKIN LEISHMANIASIS; SPECIES IDENTIFICATION; TUNISIA;

ANIMALS; DNA, PROTOZOAN; HUMANS; LEISHMANIA; LEISHMANIASIS; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SEQUENCE ANALYSIS, DNA; SPECIES SPECIFICITY; TRANSITION TEMPERATURE; TUNISIA;

LEISHMANIA INFANTUM; LEISHMANIA KILLICKI; LEISHMANIA MAJOR;

EID: 78649694062     PISSN: 00359203     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.trstmh.2010.09.003     Document Type: Article

References (36)

1. Dejeux P. Leishmaniasis: current situation and new perspectives. Comp Immunol Microbiol Infect Dis 2004, 27:305.
2. Pearson R.D., de Queiroz Sousa A. Clinical spectrum of leishmaniasis. Clin Infec Dis 1996, 22:1-13.
3. Hadighi R., Mohebali M., Boucher P., Hajjaran H., Khamesipour A., Ouellette M. Unresponsiveness to glucantime treatment in Iranian cutaneous leishmaniasis due to drug-resistant Leishmania tropica parasites. PloS Med 2006, 3:e162.
4. Rioux J.A., Lanotte G., Serre E., Pratlong F., Bastien P., Perieres J. Taxonomy of Leishmania. Use of isoenzymes. Suggestions for new classification. Ann Parasitol Hum Comp 1990, 65:111-125.
5. Aoun K., Amri F., Chouihi E., Haouas N., Bedoui K., Benikhlef R., et al. Epidemiology of Leishmania (L.) infantum, L. major and L. killicki in Tunisia: results and analysis of the identification of 226 human and canine isolates. Bull Soc Pathol Exot 2008, 101:323-328.
6. Wortmann G., Hochberg L., Houng H.H., Sweeney C., Zapor M., Aronson N., et al. Rapid identification of Leishmania complexes by a real-time PCR assay. Am J Trop Med Hyg 2005, 73:999-1004.
7. Jirkù M., Zemanová E., Al-Jawabreh A., Schönian G., Lukes J. Development of a direct species-specific PCR assay for differential diagnosis of Leishmania tropica. Diagn Microbiol Infect Dis 2006, 55:75-79.
8. Belkaïd M., Harrat Z., Hemrioui B., Thellier M., Datry A., et Danis M. A propos d'un milieu simple pour l'isolement et la culture des leishmanies. Bull Soc Pathol Exo 1996, 89:276-277.
9. Aoun K., Bouratbine A., Harrat Z., Guizani I., Mokni M., Bel Hadj Ali S., et al. pidemiologic and parasitologic data concerning sporadic cutaneous leishmaniasis in northern Tunisia. Bull Soc Pathol Exot 2000, 93:101-103.
10. Rodrigues E.H., Felinto de Brito M.E., Mendonça M.G., Werkhäuser R.P., Coutinho E.M., Souza W.V., et al. Evaluation of PCR for diagnosis of American cutaneous leishaniasis in an area of endemicity in northeastern Brazil. J Clin Microbiol 2002, 40:3572-3576.
11. Nicolas L., Milon G., Prina N. Rapid differentiation of Old World Leishmania species by LightCycler polymerase chain reaction and melting curve analysis. J Microbiol Methods 2002, 51:295-299.
12. Oliveira F.S., Pirmez C., Pires M.Q., Brazil R.P., Pacheco R.S. PCR-based diagnosis for detection of Leishmania in skin and blood of rodents from an endemic area of cutaneous and visceral leishmaniasis in Brazil. Vet Parasitol 2005, 129:219-227.
13. Al-Jawabreh A., Schoenian G., Hamarsheh O., Presber W. Clinical diagnosis of cutaneous leishmaniasis: A comparison study between standardized graded direct microscopy and ITS-PCR of a Giemsa-stained smears. Acta Tropica 2006, 99:55-61.
14. Toz S.O., Nasereddin A., Ozbel Y., Ertabaklar H., Culha G., Sevil N., et al. Leishmaniasis in Turkey: molecular characterization of Leishmania from human and canine clinical samples. Trop Med Int Health 2009, 14:1401-1406.
15. Schönian G., Nasereddine A., Dinse N., Schweynoch C., Schallig H.D., Presber W., et al. PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis 2003, 47:349-358.
16. Marfurt J., Nasereddin A., Niederwieser I., Jaffe C.L., Beck H.P., Felger I. Identification and differentiation of Leishmania species in clinical samples by PCR amplification of the miniexon sequence and subsequent restriction fragment length polymorphism analysis. J Clin Microbiol 2003, 41:3147-3153.
17. Gadisa E., Genetu A., Kuru T., Jirata D., Dagne K., Aseffa A., et al. Leishmania (Kinetoplastida): species typing with isoenzyme and PCR-RFLP from cutaneous leishmaniasis patients in Ethiopia. Exp Parasitol 2007, 115:339-343.
18. Khatri M.L., Di Muccio T., Gramiccia M. Cutaneous leishmaniasis in North-Western Yemen: a clinicoepidemiologic study and Leishmania species identification by polymerase chain reaction-restriction fragment length polymorphism analysis. J Am Acad Dermatol 2009, 61:e15-21.
19. El Tai N.O., El Fari M., Mauricio I., Miles M.A., Oskam L., El Safi S., et al. Leishmania donovani: intraspecific polymorphisms of Sudanese isolates revealed by PCR based analyses and DNA sequencing. Exper Parasitol 2001, 97:35-44.
20. Mihoubi I., de Monbrison F., Romeuf N., Moulahem T., Picot Outsourced real-time PCR diagnosis of cutaneous leishmaniasis in the outbreak region of Constantine, Algeria. Algeria Med Trop 2006, 66:39-44.
21. Monbrison de F., Mihoubi I., Picot S. Real-time PCR assay for the identification of cutaneous Leishmania parasite species in Constantine region of Algeria. Acta Trop 2007, 102:79-83.
22. Talmi-Frank D., Nasereddin A., Schnur L.F., Schönian G., Töz S.O., Jaffe C.L., et al. Detection and identification of old world Leishmania by high resolution melt analysis. PloS Negl Trop Dis 2010, 4:e581.
23. Chouihi E., Amri F., Bouslimi N., Siala E., Selmi K., Zallagua N., et al. Cultures on NNN medium for the diagnosis of leishmaniasis. Pathol Biol 2009, 57:219-224.
24. Van Eys G.J., Schoone G.J., Kroon N.C., Ebeling S.B. Sequence analysis of small subunit ribosomal RNA genes and its use for detection and identification of Leishmania parasites. Mol Biochem Parasitol 1992, 51:133-142.
25. Russell R., Iribar M.P., Lambson B., Brewster S., Blackwell J.M., Dye C., et al. Intra and inter-specific microsatellite variation in the Leishmania subgenus. Viannia Mol Biochem Parasitol 1999, 103:71-77.
26. Brewster S., Barker D.C. Analysis of minicircle classes in Leishmania (Viannia) species. Trans R Soc Trop Med Hyg 2002, 96:S55-63.
27. Anders G., Eisenberger C.L., Jonas F., Greenblatt C.L. Distinguishing Leishmania tropica and Leishmania major in the Middle East using the polymerase chain reaction with kinetoplast DNA-specific primers. Trans R Soc Trop Med Hyg 2002, 96:87-92.
28. Kumar R., Bumb R.A., Ansari N.A., Mehta R.D., Salotra P. Cutaneous leishmaniasis caused by Leishmania tropica in Bikaner, India: parasite identification and characterization using molecular and immunologic tools. Am J Trop Med Hyg 2007, 76:896-901.
29. Haouas N., Gorcii M., Chargui N., Aoun K., Bouratbine A., Messaadi Akrout F., et al. Leishmaniasis in central and southern Tunisia: current geographical distribution of zymodemes. Parasite 2007, 14:239-246.
30. Bouratbine A., Aoun K., Ghrab J., Harrat Z., Ezzedini M.S., Etlijani S. Spread of Leishmania killicki to Central and South-West Tunisia. Parasite 2005, 12:59-63.
31. Lemrani M., Hamdi S., Laamrani A., Hassar M. PCR detection of Leishmania in skin biopsies. J Infect Dev Ctries 2009, 3:115-122.
32. Bhattarai N.R., Dujardin J.C., Rijal S., De Doncker S., Boelaert M., Van der Auwera G. Development and evaluation of different PCR-based typing methods for discrimination of Leishmania donovani isolates from Nepal. Parasitology 2010, 137:947-957.
33. Nasereddin A., Azmi K., Jaffe C.L., Ereqat S., Amro A., Sawalhah G., et al. Kinetoplast DNA heterogeneity among L. infantum strains in central Israel and Palastine. Vet Parasitol 2009, 161:126-130.
34. Schönian G., Schnur L., El Fari M., Oskam L., Kolesnikov A.A., Sokolowska-Köhler W., et al. Genetic heterogeneity in the species Leishmania tropica revealed by different PCR-based methods. Trans R Soc Trop Med Hyg 2001, 95:217-224.
35. Schwenkenbecher J.M., Wirth T., Schnur L.F., Jaffe C.L., Schallig H., Al Jawabreh A., et al. Microsatellite analysis reveals genetic structure of Leishmania tropica. Int J Parasitol 2006, 36:237-246.
36. Bastien P., Procop G.W., Reischl U. Quantitative real-time PCR is not more sensitive than " conventional" PCR. J Clin Microbiol 2008, 46:1897-1900.