Highly effective removal of floxed Blasticidin S resistance cassettes from Dictyostelium discoideum mutants by extrachromosomal expression of Cre

European Journal of Cell Biology

Volumn 91, Issue 2, 2012, Pages 156-160

  Linkner, Joern ^a   Nordholz, Benjamin ^a   Junemann, Alexander ^a   Winterhoff, Moritz ^a   Faix, Jan ^a  

^a HANNOVER MEDICAL SCHOOL   (Germany)

Author keywords

Cre recombinase; Dictyostelium discoideum; Extrachromosomal plasmid knockout; LoxP sites

Indexed keywords

BLASTICIDIN S; CRE RECOMBINASE; NEOMYCIN;

ARTICLE; CELL LINE; CONTROLLED STUDY; DICTYOSTELIUM DISCOIDEUM; EXPRESSION VECTOR; GENE CASSETTE; GENE INACTIVATION; GENOME; HOMOLOGOUS RECOMBINATION; LOXP SITE; MUTANT; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; TRANSIENT EXPRESSION;

CELL LINE; DICTYOSTELIUM; GENE EXPRESSION; GENE KNOCKOUT TECHNIQUES; GENETIC ENGINEERING; GENETIC MARKERS; GENETIC VECTORS; HOMOLOGOUS RECOMBINATION; INTEGRASES; MUTAGENESIS, INSERTIONAL; NUCLEOSIDES; ORGANISMS, GENETICALLY MODIFIED; TRANSFECTION;

DICTYOSTELIUM DISCOIDEUM;

EID: 84855540299     PISSN: 01719335     EISSN: 16181298     Source Type: Journal    
DOI: 10.1016/j.ejcb.2011.11.001     Document Type: Article

References (32)

1. Austin C.P., Battey J.F., Bradley A., Bucan M., Capecchi M., Collins F.S., Dove W.F., Duyk G., Dymecki S., Eppig J.T., et al. The knockout mouse project. Nat. Genet. 2004, 36:921-924.
2. Barth C., Fraser D.J., Fisher P.R. A rapid, small scale method for characterization of plasmid insertions in the Dictyostelium genome. Nucl. Acids Res. 1998, 26:3317-3318.
3. Blangy A., Lane H.A., d'Herin P., Harper M., Kress M., Nigg E.A. Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo. Cell 1995, 83:1159-1169.
4. Bolourani P., Spiegelman G.B., Weeks G. Delineation of the roles played by RasG and RasC in cAMP-dependent signal transduction during the early development of Dictyostelium discoideum. Mol. Biol. Cell 2006, 17:4543-4550.
5. Breitsprecher D., Kiesewetter A.K., Linkner J., Vinzenz M., Stradal T.E., Small J.V., Curth U., Dickinson R.B., Faix J. Molecular mechanism of Ena/VASP-mediated actin-filament elongation. EMBO J. 2011, 30:456-467.
6. De Lozanne A. Homologous recombination in Dictyostelium as a tool for the study of developmental genes. Methods Cell Biol. 1987, 28:489-495.
7. Derivery E., Lombard B., Loew D., Gautreau A. The Wave complex is intrinsically inactive. Cell Motil. Cytoskel. 2009, 66:777-790.
8. Egelhoff T.T., Lee R.J., Spudich J.A. Dictyostelium myosin heavy chain phosphorylation sites regulate myosin filament assembly and localization in vivo. Cell 1993, 75:363-371.
9. Eichinger L., Pachebat J.A., Glockner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., et al. The genome of the social amoeba Dictyostelium discoideum. Nature 2005, 435:43-57.
10. Faix J., Kreppel L., Shaulsky G., Schleicher M., Kimmel A.R. A rapid and efficient method to generate multiple gene disruptions in Dictyostelium discoideum using a single selectable marker and the Cre-loxP system. Nucl. Acids Res. 2004, 32:e143.
11. Fire A., Xu S., Montgomery M.K., Kostas S.A., Driver S.E., Mello C.C. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 1998, 391:806-811.
12. Han Y.H., Chung C.Y., Wessels D., Stephens S., Titus M.A., Soll D.R., Firtel R.A. Requirement of a vasodilator-stimulated phosphoprotein family member for cell adhesion, the formation of filopodia, and chemotaxis in Dictyostelium. J. Biol. Chem. 2002, 277:49877-49887.
13. Hughes J.E., Kiyosawa H., Welker D.L. Plasmid maintenance functions encoded on Dictyostelium discoideum nuclear plasmid Ddp1. Mol. Cell. Biol. 1994, 14:6117-6124.
14. Kimmel A.R., Faix J. Generation of multiple knockout strains using the Cre-loxP system. Methods Mol. Biol. 2006, 346:187-199.
15. King J.S., Insall R.H. Chemotaxis: finding the way forward with Dictyostelium. Trends Cell Biol. 2009, 19:523-530.
16. Kwiatkowski A.V., Rubinson D.A., Dent E.W., Edward van Veen J., Leslie J.D., Zhang J., Mebane L.M., Philippar U., Pinheiro E.M., Burds A.A., et al. Ena/VASP is required for neuritogenesis in the developing cortex. Neuron 2007, 56:441-455.
17. Lee E., Knecht D.A. Visualization of actin dynamics during macropinocytosis and exocytosis. Traffic 2002, 3:186-192.
18. Leiting B., Noegel A. Construction of an extrachromosomally replicating transformation vector for Dictyostelium discoideum. Plasmid 1988, 20:241-248.
19. Levi S., Polyakov M., Egelhoff T.T. Green fluorescent protein and epitope tag fusion vectors for Dictyostelium discoideum. Plasmid 2000, 44:231-238.
20. Linkner J., Witte G., Stradal T., Curth U., Faix J. High-resolution X-ray structure of the trimeric Scar/WAVE-complex precursor Brk1. PLoS One 2011, e21327.
21. Ma Y., Creanga A., Lum L., Beachy P.A. Prevalence of off-target effects in Drosophila RNA interference screens. Nature 2006, 443:359-363.
22. Odell G.M., Bonner J.T. How the Dictyostelium discoideum grex crawls. Phil. Trans. R. Soc. Lond. B 1986, 312:487-525.
23. Pollitt A.Y., Blagg S.L., Ibarra N., Insall R.H. Cell motility and SCAR localisation in axenically growing Dictyostelium cells. Eur. J. Cell Biol. 2006, 85:1059-1068.
24. Schirenbeck A., Arasada R., Bretschneider T., Stradal T.E.B., Schleicher M., Faix J. The bundling activity of vasodilator-stimulated phosphoprotein is required for filopodium formation. Proc. Natl. Acad. Sci. U.S.A. 2006, 103:7694-7699.
25. Schirenbeck A., Bretschneider T., Arasada R., Schleicher M., Faix J. The Diaphanous-related formin dDia2 is required for the formation and maintenance of filopodia. Nat. Cell Biol. 2005, 7. 619-U24.
26. Steffen A., Faix J., Resch G.P., Linkner J., Wehland J., Small J.V., Rottner K., Stradal T.E.B. Filopodia formation in the absence of functional WAVE- and Arp2/3-complexes. Mol. Biol. Cell 2006, 17:2581-2591.
27. Straight A.F., Cheung A., Limouze J., Chen I., Westwood N.J., Sellers J.R., Mitchison T.J. Dissecting temporal and spatial control of cytokinesis with a myosin II inhibitor. Science 2003, 299:1743-1747.
28. Sutoh K. A transformation vector for Dictyostelium discoideum with a new selectable marker bsr. Plasmid 1993, 30:150-154.
29. Vicker M.G. F-actin assembly in Dictyostelium cell locomotion and shape oscillations propagates as a self-organized reaction-diffusion wave. FEBS Lett. 2002, 510:5-9.
30. Williams J.G. Transcriptional regulation of Dictyostelium pattern formation. EMBO Rep. 2006, 7:694-698.
31. Witke W., Nellen W., Noegel A. Homologous recombination in the Dictyostelium alpha-actinin gene leads to an altered mRNA and lack of the protein. EMBO J. 1987, 6:4143-4148.
32. Yaniv M., Cereghini S. Structure of transcriptionally active chromatin. CRC Crit. Rev. Biochem. 1986, 21:1-26.