Large-scale production of saikosaponins through root culturing of Bupleurum falcatum L. using modified airlift reactors

Journal of Bioscience and Bioengineering

Volumn 113, Issue 1, 2012, Pages 99-105

  Kusakari, Ken ^a,b   Yokoyama, Mineyuki ^a   Inomata, Shinji ^a   Gozu, Yoko ^a   Katagiri, Chika ^a   Sugimoto, Yukihiro ^b  

^a SHISEIDO RESEARCH CENTER   (Japan)

^b KOBE UNIVERSITY   (Japan)

Author keywords

Airlift reactor; Bupleurum falcatum L.; Large scale culture; Root culture; Saikosaponin

Indexed keywords

AIR-LIFT REACTORS; BUBBLE COLUMN REACTORS; BUPLEURUM FALCATUM L.; CULTURE MEDIUM; DRAFT TUBES; EMPTY SPACE; LARGE-SCALE PRODUCTION; LOW AERATION RATES; ROOT CULTURES; ROOT YIELD; ROTATION SPEED; SAIKOSAPONINS; SOLID MATERIAL; STAINLESS STEEL MESH; STIRRED TANK REACTORS;

BUBBLE COLUMNS; FLOCCULATION; HYDRAULIC TURBINES; STAINLESS STEEL; TUBES (COMPONENTS);

PERFORATED PLATES;

SAIKOSAPONIN A; SAIKOSAPONIN D;

AERATION; AIRLIFT REACTOR; ARTICLE; BUPLEURUM FALCATUM; CHEMICAL REACTION; CONTROLLED STUDY; CULTURE MEDIUM; FLOCCULATION; FOAMING; MEDICINAL PLANT; PLANT GROWTH; PLANT ROOT; STIRRED REACTOR;

BIOREACTORS; BUPLEURUM; CULTURE MEDIA; OLEANOLIC ACID; PLANT ROOTS; SAPONINS; TISSUE CULTURE TECHNIQUES;

BUPLEURUM FALCATUM;

EID: 84855342144     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2011.08.019     Document Type: Article

References (48)

1. Wang Y. Chai-hu. Pharmacology and Applications of Chinese Materia Medica 1987, vol. 2:978-988. World Scientific, New Jersey. H.M. Chang, P.P.H. But (Eds.).
2. Ishii H., Nakamura M., Seo S., Tori K., Tozyo T., Yoshimura Y. Isolation, characterization, and nuclear magnetic resonance spectra of new saponins from the roots of Bupleurum falcatum L. Chem. Pharm. Bull. 1980, 28:2367-2373.
3. Park K.H., Park J., Koh D., Lim Y. Effect of saikosaponin-A, a triterpenoid glycoside, isolated from Bupleurum falcatum on experimental allergic asthma. Phytother. Res. 2002, 16:359-363.
4. Yamamoto M., Kumagai A., Yamamura Y. Structure and actions of saikosaponins isolated from Bupleurum falcatum L. I. Anti-inflammatory action of saikosaponins. Arzneim. Forsch. 1975, 25:1021-1023.
5. Yamamoto M., Kumagai A., Yamamura Y. Structure and actions of saikosaponins isolated from Bupleurum falcatum L. II. Metabolic actions of saikosaponins, especially a plasma cholesterol-lowering action. Arzneim. Forsch. 1975, 25:1240-1243.
6. Kita T., Hata T., Ito E., Yoneda R. Analgesic and other pharmacologic actions of saikosaponin in repeated cold stressed (SART stressed) animals. J. Pharm. Dyn. 1980, 3:269-280.
7. Kato M., Pu M.Y., Isobe K., Iwamoto T., Nagase F., Lwin T., Zhang Y.H., Hattori T., Yanagita N., Nakashima I. Characterization of the immunoregulatory action of saikosaponin-d. Cell. Immunol. 1994, 159:15-25.
8. Fan J., Li X., Li P., Li N., Wang T., Shen H., Siow Y., Choy P., Gong Y. Saikosaponin-d attenuates the development of liver fibrosis by preventing hepatocyte injury. Biochem. Cell Biol. 2007, 85:189-195.
9. Shimizu K., Amagaya S., Ogihara Y. Structural transformation of saikosaponins by gastric juice and intestinal flora. J. Pharmacobiodyn. 1985, 8:718-725.
10. Nishiyama T., Akutsu N., Nakayama Y. Effects of saikosaponin b1 on the synthesis of VII type collagen. Seikagaku 1999, 71(889). (in Japanese).
11. Shimokawa Y., Okuda I., Kuwano M., Ohashi H. Cultivation and breeding of Bupleurum falcatum L. (VI) Geographical variation of B. falcatum. Shoyakugaku Zasshi 1980, 34:239-244. (in Japanese).
12. Wilson P.D.G., Hilton M.G., Robins R.J., Rhodes J.C. Fermentation studies of transformed root cultures. Bioreactors and Biotransformations 1987, 38-51. Elsevier Applied Science Publishers, London New York. G.W. Moody, P.B. Baker (Eds.).
13. Hashimoto T., Yamada Y. Organ culture and manipulation. Methods in Plant Biochemistry 1991, vol. 6:323-350. Academic Press, London. K. Hostettmann (Ed.).
14. Son S.H., Choi S.M., Lee Y.H., Choi K.B., Yun S.R., Kim J.K., Park H.J., Kwon O.W., Noh E.W., Seon J.H., Park Y.G. Large-scale growth and taxane production in cell cultures of Taxus cuspidata (Japanese yew) using a novel bioreactor. Plant Cell Rep. 2000, 19:628-633.
15. Uomori A., Seo S., Tomita Y. Studies on the constituents in tissue cultures of Bupleurum falcatum L. Shoyakugaku Zasshi 1974, 28:152-160. (in Japanese).
16. Wang P.J., Huang C.I. Production of saikosaponins by callus and redifferentiated organs of Bupleurum falcatum L. Plant Tissue Culture 1982, 71-72. Maruzen, Tokyo. A. Fujiwara (Ed.).
17. Yamamoto O., Kamura K. Production of saikosaponin in cultured roots of Bupleurum falcatum L. Plant Tissue Cult. Biotechnol. 1997, 3:138-147.
18. Kusakari K., Yokoyama M., Inomata S. Enhanced production of saikosaponins by root culture of Bupleurum falcatum L. using two-step control of sugar concentration. Plant Cell Rep. 2000, 19:1115-1120.
19. Aoyagi H., Kobayashi Y., Yamada K., Yokoyama M., Kusakari K., Tanaka H. Efficient production of saikosaponins in Bupleurum falcatum root fragments combined with signal transducers. Appl. Microbiol. Biotechnol. 2001, 57:482-488.
20. Ahn J.C., Chong W.S., Kim Y.S., Hwang B. Optimization of the sucrose and ion concentrations for saikosaponin production in hairy root culture of Bupleurum falcatum. Biotechnol. Bioprocess Eng. 2006, 11:121-126.
21. Eibl R., Eibl D. Design of bioreactors suitable for plant cell and tissue cultures. Phytochem. Rev. 2008, 7:593-598.
22. Hilton M.G., Rhodes M.J.C. Growth and hyoscyamine production of 'hairy root' cultures of Datura stramonium in a modified stirred tank reactor. Appl. Microbiol. Biotechnol. 1990, 33:132-138.
23. Uozumi N., Kohketsu K., Kondo O., Honda H., Kobayashi T. Fed-batch culture of hairy root using fructose as a carbon source. J. Ferment. Bioeng. 1991, 72:457-460.
24. Inomata S., Yokoyama M., Gozu Y., Shimizu T., Yanagi M. Growth pattern and ginsenoside production of Agrobacterium-transformed Panax ginseng roots. Plant Cell Rep. 1993, 12:681-686.
25. Tanaka H., Nishijima F., Suwa M., Iwamoto T. Rotating drum fermentor for plant cell suspension culture. Biotechnol. Bioeng. 1983, 25:2359-2370.
26. Kondo O., Honda H., Taya M., Kobayashi T. Comparison of growth properties of carrot hairy root in various bioreactors. Appl. Microbiol. Biotechnol. 1989, 32:291-294.
27. Carvalho E.B., Curtis W.R. Characterization of fluid-flow resistance in root cultures with a convective flow tubular bioreactor. Biotechnol. Bioeng. 1998, 60:375-384.
28. Kim Y., Wyslouzil B.E., Weathers P.J. A comparative study of mist and bubble column reactors in the in vitro production of artemisinin. Plant Cell Rep. 2001, 20:451-455.
29. Liu C.Z., Towler M.J., Medrano G., Cramer C.L., Weathers P.J. Production of mouse interleukin-12 is greater in tobacco hairy roots grown in a mist reactor than in an airlift reactor. Biotechnol. Bioeng. 2008, 102:1074-1086.
30. Choi S.M., Son S.H., Yun S.R., Kwon O.W., Seon J.H., Paek K.Y. Pilot-scale culture of adventitious roots of ginseng in a bioreactor system. Plant Cell Tissue Organ Cult. 2000, 62:187-193.
31. Choi Y.E., Lee K.S., Kim E.Y., Kim Y.S., Han J.Y., Kim H.S., Jeong J.H., Ko S.K. Mass production of Siberian ginseng plantlets through large-scale tank culture of somatic embryos. Plant Cell Rep. 2002, 21:24-28.
32. Linsmaier E.M., Skoog F. Organic growth factor requirements of tobacco tissue culture. Physiol. Plant. 1965, 18:100-127.
33. Gamborg O.L., Miller R.A., Ojima K. Nutrient requirements of suspension culture of soybean root cells. Exp. Cell Res. 1968, 50:151-158.
34. Ramakrishnan D., Curtis W.R. Fluid dynamic studies on plant root cultures for application to bioreactor design. Advances in Plant biotechnology. Studies in plant science 1994, vol. 4:281-305. Elsevier, Amsterdam. D.D.Y. Ryu, S. Furusaki (Eds.).
35. Murakami S., Chiou T.W., Wang D.I.C. A fiber-bed bioreactor for anchourage-dependent animal cell cultures: part II. Scaleup potential. Biotechnol. Bioeng. 1991, 37:762-769.
36. Jung G., Tepfer D. Use of genetic transformation by Ri T-DNA of Agrobacterium rhizogenes to stimulate biomass and alkaloid production in Atropa belladonna and Calystegia sepium roots grown in vitro. Plant Sci. 1987, 50:145-151.
37. Kim Y.H., Yoo Y.J. Development of a bioreactor for high density culture of hairy roots. Biotechnol. Tech. 1993, 7:859-862.
38. Paek K.Y., Chakrabarty D., Hahn E.J. Application of bioreactor systems for large scale production of horticultural and medicinal plants. Plant Cell Tissue Organ Cult. 2005, 81:287-300.
39. Wu C.H., Murthy H.N., Hahn E.J., Paek K.Y. Large-scale cultivation of adventitious roots of Echinacea purpurea in airlift bioreactors for the production of chichoric acid, chlorogenic acid and caftaric acid. Biotechnol. Lett. 2007, 29:1179-1182.
40. Joseong J.A., Wu C.H., Murthy H.N., Hahn E.J., Paek K.Y. Application of an airlift bioreactor system for the production of adventitious root biomass and caffeic acid derivatives of Echinacea purpurea. Biotechnol. Bioprocess Eng. 2009, 14:91-98.
41. Taya M., Yoyoma A., Kondo O., Kobayashi T., Matsui C. Growth characteristics of plant hairy roots and their cultures in bioreactors. J. Chem. Eng. Jpn. 1989, 22:84-89.
42. Rodriguez-Mendiola M.A., Stafford A., Cresswell R., Arias-Castro C. Bioreactors for growth of plant roots. Enzyme Microb. Technol. 1991, 13:697-702.
43. Caspeta L., Nieto I., Zamilpa A., Alvarez L., Quintero R., Villarreal L.L. Solanum chrysotrichum hairy root cultures: characterization, scaleup and production of five antifungal saponins for human use. Planta Med. 2005, 71:1084-1087.
44. Abbasi B.H., Liu R., Saxena P.K., Liu C.Z. Cichoric acid production from hairy root cultures of Echinacea purpurea grown in a modified airlift bioreactor. J. Chem. Technol. Biotechnol. 2009, 84:1697-1701.
45. Zhong J.J., Pan Z.W., Wang Z.Y., Wu J.Y., Chen F., Takagi M., Yoshida T. Effect of mixing time on taxoid production in suspension cultures of Taxus chinensis in a centrifugal impeller bioreactor. J. Biosci. Bioeng. 2002, 94:244-250.
46. Hu W.W., Zhong J.J. Effect of bottom clearance on performance of airlift bioreactor in high-density culture of Panax notoginseng cells. J. Biosci. Bioeng. 2001, 92:389-392.
47. Kim Y.S., Cho J.H., Ahn J., Hwang B. Upregulation of isoprenoid pathway genes during enhanced saikosaponin biosynthesis in the hairy roots of Bupleurum falcatum. Mol. Cells 2006, 22:269-274.
48. Kim Y.S., Cho J.H., Park S., Han J.Y., Back K., Choi Y.E. Gene regulation patterns in triterpene biosynthetic pathway driven by overexpression of squalene synthase and methyl jasmonate elicitation in Bupleurum falcatum. Planta 2010, 233:343-355.