Hepatitis B virus X protein mutant upregulates CENP-A expression in hepatoma cells

Oncology Reports

Volumn 27, Issue 1, 2012, Pages 168-173

  Liu, Lina ^a,d   Li, Yongmei ^a   Zhang, Shuhui ^c   Yu, Danghui ^a   Zhu, Minghua ^a,b  

^a SECOND MILITARY MEDICAL UNIVERSITY   (China)

^b SECOND MILITARY MEDICAL UNIVERSITY   (China)

^c SHANGHAI UNIVERSITY OF TRADITIONAL CHINESE MEDICINE   (China)

^d Shanghai Hospital of Armed Police Corps   (China)

Author keywords

Centromere protein A; Hepatitis B virus X protein; Hepatocellular carcinoma

Indexed keywords

CENTROMERE PROTEIN A; CYCLIN DEPENDENT KINASE INHIBITOR 1; CYCLIN DEPENDENT KINASE INHIBITOR 2A; GENOMIC DNA; HEPATITIS B ANTIGEN; HEPATITIS B VIRUS X PROTEIN; MESSENGER RNA; PROTEIN MDM2; PROTEIN P53;

ADULT; AGED; ARTICLE; CANCER TISSUE; CARBOXY TERMINAL SEQUENCE; CELL STRAIN HEPG2; CLINICAL ARTICLE; CONTROLLED STUDY; DELETION MUTANT; FEMALE; GENE DELETION; GENE EXPRESSION REGULATION; GENE MUTATION; GENE OVEREXPRESSION; GENE SEQUENCE; HEPATITIS B VIRUS; HEPATITIS B VIRUS X PROTEIN GENE; HUMAN; HUMAN CELL; HUMAN TISSUE; IMMUNOREACTIVITY; LIVER CARCINOGENESIS; LIVER CELL CARCINOMA; MALE; MISSENSE MUTATION; NONSENSE MUTATION; NUCLEOTIDE SEQUENCE; POINT MUTATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; SENSE MUTATION; UPREGULATION; VIRUS CARCINOGENESIS; VIRUS GENE;

ADULT; AGED; AUTOANTIGENS; BASE SEQUENCE; BLOTTING, WESTERN; CARCINOMA, HEPATOCELLULAR; CELL LINE, TUMOR; CHROMOSOMAL PROTEINS, NON-HISTONE; FEMALE; HEP G2 CELLS; HUMANS; IMMUNOHISTOCHEMISTRY; IMMUNOPRECIPITATION; LIVER NEOPLASMS; MALE; MIDDLE AGED; MOLECULAR SEQUENCE DATA; MUTATION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TRANS-ACTIVATORS; UP-REGULATION;

EID: 84855296445     PISSN: 1021335X     EISSN: 17912431     Source Type: Journal    
DOI: 10.3892/or.2011.1478     Document Type: Article

References (32)

1. Boyle P and Levin B: World Cancer Report 2008. WHO Press, Geneva, p350, 2009.
2. Farazi PA and DePinho RA: Hepatocellular carcinoma pathogenesis: from genes to environment. Nat Rev Cancer 6: 674-687, 2006. (Pubitemid 44286000)
3. Fan CG, Wang CM, Tian C, et al: miR-122 inhibits viral replication and cell proliferation in hepatitis B virus-related hepatocellular carcinoma and targets NDRG3. Oncol Rep 26: 1281-1286, 2011.
4. Thorgeirsson SS and Grisham JW: Molecular pathogenesis of human hepatocellular carcinoma. Nat Genet 31: 339-346, 2002. (Pubitemid 35154440)
5. Sun HC, Li M, Lu JL, et al: Overexpression of Forkhead box M1 protein associates with aggressive tumor features and poor prognosis of hepatocellular carcinoma. Oncol Rep 25: 1533-1539, 2011.
6. Tu H, Bonura C, Giannini C, et al: Biological impact of natural COOH-terminal deletions of hepatitis B virus X protein in hepatocellular carcinoma tissues. Cancer Res 61: 7803-7810, 2001. (Pubitemid 33049368)
7. Liu X, Wang L, Zhang S, et al: Mutations in the C-terminus of the X protein of hepatitis B virus regulate Wnt-5a expression in hepatoma Huh7 cells: cDNA microarray and proteomic analyses. Carcinogenesis 29: 1207-1214, 2008. (Pubitemid 351958700)
8. Ng RK, Lau CY, Lee SM, Tsui SK, Fung KP and Waye MM: cDNA microarray analysis of early gene expression profiles associated with hepatitis B virus X protein-mediated hepatocarcinogenesis. Biochem Biophys Res Commun 322: 827-835, 2004. (Pubitemid 39164427)
9. Wang Y, Lau SH, Sham JS, Wu MC, Wang T and Guan XY: Characterization of HBV integrants in 14 hepatocellular carcinomas: association of truncated X gene and hepatocellular carcinogenesis. Oncogene 23: 142-148, 2004. (Pubitemid 38142248)
10. Hsia CC, Nakashima Y and Tabor E: Deletion mutants of the hepatitis B virus X gene in human hepatocellular carcinoma. Biochem Biophys Res Commun 241: 726-729, 1997. (Pubitemid 28036725)
11. Iavarone M, Trabut JB, Delpuech O, et al: Characterisation of hepatitis B virus X protein mutants in tumour and non-tumour liver cells using laser capture microdissection. J Hepatol 39: 253-261, 2003. (Pubitemid 36920562)
12. Prystowsky MB, Adomako A, Smith RV, et al: The histone deacetylase inhibitor LBH589 inhibits expression of mitotic genes causing G2/M arrest and cell death in head and neck squamous cell carcinoma cell lines. J Pathol 218: 467-477, 2009.
13. Liu X, Zhang S, Lin J, et al: Hepatitis B virus X protein mutants exhibit distinct biological activities in hepatoma Huh7 cells. Biochem Biophys Res Commun 373: 643-647, 2008.
14. Li YM, Zhu Z, Chen Y, Luo ZG, Shi M and Zhu MH: Effect of siRNA targeting centromere protein-A gene on biological behavior of HepG2 cells. Chin J Pathol 37: 124-128, 2008. (Pubitemid 351500731)
15. Malone JL, Nelson AC, Lieberman R, Anderson S and Holt JT: Oestrogen-mediated phosphorylation and stabilization of BRCA2 protein in breast. J Pathol 217: 380-388, 2009.
16. Li Y, Zhu Z, Zhang S, et al: ShRNA-targeted centromere protein A inhibits hepatocellular carcinoma growth. PLoS One 6: e17794, 2011.
17. Hamilton S and Aaltonen L: World Health Organization Classification of Tumours: Pathology and genetics of tumours of the digestive system. IARC Press, Lyon, pp167-169, 2000.
18. Minamishima YA, Nakayama K and Nakayama K: Recovery of liver mass without proliferation of hepatocytes after partial hepatectomy in Skp2-deficient mice. Cancer Res 62: 995-999, 2002. (Pubitemid 34160276)
19. Zhu Z, Luo Z, Li Y, Ni C, Li H and Zhu M: Human inhibitor of growth 1 inhibits hepatoma cell growth and influences p53 stability in a variant-dependent manner. Hepatology 49: 504-512, 2009.
20. Xian ZH, Zhang SH, Cong WM, Yan HX, Wang K and Wu MC: Expression of aspartyl beta-hydroxylase and its clinicopathological significance in hepatocellular carcinoma. Mod Pathol 19: 280-286, 2006. (Pubitemid 43117613)
21. Pfaffl MW: A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29: e45, 2001.
22. Zeindl-Eberhart E, Haraida S, Liebmann S, et al: Detection and identification of tumor-associated protein variants in human hepatocellular carcinomas. Hepatology 39: 540-549, 2004. (Pubitemid 38228380)
23. Zeindl-Eberhart E, Klugbauer S, Dimitrijevic N, Jungblut PR, Lamer S and Rabes HM: Proteome analysis of rat hepatomas: carcinogen-dependent tumor-associated protein variants. Electrophoresis 22: 3009-3018, 2001.
24. Liu XH, Lin J, Zhang SH, et al: COOH-terminal deletion of HBx gene is a frequent event in HBV-associated hepatocellular carcinoma. World J Gastroenterol 14: 1346-1352, 2008. (Pubitemid 351425212)
25. Regnier V, Vagnarelli P, Fukagawa T, et al: CENP-A is required for accurate chromosome segregation and sustained kinetochore association of BubR1. Mol Cell Biol 25: 3967-3981, 2005. (Pubitemid 40617647)
26. Blower MD and Karpen GH: The role of Drosophila CID in kinetochore formation, cell-cycle progression and heterochromatin interactions. Nat Cell Biol 3: 730-739, 2001. (Pubitemid 32734251)
27. Maehara K, Takahashi K and Saitoh S: CENP-A reduction induces a p53-dependent cellular senescence response to protect cells from executing defective mitoses. Mol Cell Biol 30: 2090-2104, 2010.
28. He Y, Sun HQ, He XE, Wang WL and Lei JH: Knockdown of HBx by RNAi inhibits proliferation and enhances chemotherapy-induced apoptosis in hepatocellular carcinoma cells. Med Oncol 27: 1227-1233, 2010.
29. Lin HK, Wang G, Chen Z, et al: Phosphorylation-dependent regulation of cytosolic localization and oncogenic function of Skp2 by Akt/PKB. Nat Cell Biol 11: 420-432, 2009.
30. Park IY, Sohn BH, Yu E, et al: Aberrant epigenetic modifications in hepatocarcinogenesis induced by hepatitis B virus X protein. Gastroenterology 132: 1476-1494, 2007. (Pubitemid 46627570)
31. Merzianu M, Jiang L, Lin P, et al: Nuclear BCL-10 expression is common in lymphoplasmacytic lymphoma/Waldenstrom macroglobulinemia and does not correlate with p65 NF-kappaB activation. Mod Pathol 19: 891-898, 2006.
32. Sirma H, Giannini C, Poussin K, Paterlini P, Kremsdorf D and Brechot C: Hepatitis B virus X mutants, present in hepatocellular carcinoma tissue abrogate both the antiproliferative and transactivation effects of HBx. Oncogene 18: 4848-4859, 1999. (Pubitemid 29423568)