Expression of soluble and functional full-length human matrix metalloproteinase-2 in Escherichia coli

Journal of Biotechnology

Volumn 157, Issue 1, 2012, Pages 20-24

  Gonçalves, Andrezza N ^a   Meschiari, Cesar A ^a   Stetler Stevenson, William G ^b   Nonato, M Cristina ^a   Alves, Cleidson P ^a   Espreafico, Enilza M ^a   Gerlach, Raquel F ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Bacteria; Escherichia coli; Gelatinase A; Matrix metalloproteinase 2; Protein expression

Indexed keywords

BIOLOGICAL ACTIVITIES; E. COLI; EUKARYOTIC PROTEINS; EUKARYOTIC TISSUE; FLUORIMETRIC ASSAYS; GELATINASE-A; HUMAN MATRIX; MATRIX METALLOPROTEINASE-2; METALLOPROTEINASES; MMP INHIBITOR; PROTEIN EXPRESSION; SOLUBLE FRACTION; WESTERN BLOTTING;

BACTERIA; CYTOLOGY; GEL PERMEATION CHROMATOGRAPHY; PROTEINS; PURIFICATION; TISSUE; TISSUE CULTURE;

ESCHERICHIA COLI;

GELATIN; GELATINASE A; RECOMBINANT MATRIX METALLOPROTEINASE 2; RECOMBINANT PROTEIN; SEPHAROSE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM CULTURE; CELL LYSATE; CULTURE MEDIUM; CYTOPLASM; ENZYME ASSAY; ENZYME PURIFICATION; ESCHERICHIA COLI; EXPRESSION VECTOR; FLUOROMETRY; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN FUNCTION; WESTERN BLOTTING; ZYMOGRAPHY;

CLONING, MOLECULAR; ESCHERICHIA COLI; HUMANS; MATRIX METALLOPROTEINASE 2; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA;

EID: 84855232393     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.09.030     Document Type: Article

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