A fluorescent-based HPLC assay for quantification of cysteine and cysteamine adducts in Escherichia coli-derived proteins

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 880, Issue 1, 2012, Pages 27-33

  Soriano, Brian D ^a   Tam, Lei Ting T ^a   Lu, Hsieng S ^a   Valladares, Violeta G ^a  

^a AMGEN INC   (United States)

Author keywords

AccQTag; Adduct; Cysteamine; Cysteine; HPLC; Mass spectrometry; Peptibody; Thiol

Indexed keywords

ACCQTAG; ADDUCT; CYSTEAMINE; CYSTEINE; HPLC; PEPTIBODY; THIOL;

AMINO ACIDS; COVALENT BONDS; ESCHERICHIA COLI; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PURIFICATION;

PROTEINS;

6 AMINOQUINOLYL N HYDROXYSUCCINIMIDYL CARBAMATE; CYSTEINE; ESCHERICHIA COLI PROTEIN; FLUORESCENT DYE; MERCAPTAMINE; THIOL DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIMIT OF QUANTITATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION;

AMINOQUINOLINES; CARBAMATES; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYSTEAMINE; CYSTEINE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FLUORESCENT DYES; LIMIT OF DETECTION; MASS SPECTROMETRY; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI;

EID: 84855186457     PISSN: 15700232     EISSN: 1873376X     Source Type: Journal    
DOI: 10.1016/j.jchromb.2011.11.011     Document Type: Article

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