Knockdown of BMI-1 causes cell-cycle arrest and derepresses p16 INK4a, HOXA9 and HOXC13 mRNA expression in HeLa cells

Medical Oncology

Volumn 28, Issue 4, 2011, Pages 1201-1209

  Chen, Fenghua ^a   Li, Yirong ^a   Wang, Lin ^a   Hu, Lihua ^a  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

B cell specific Moloney murine leukemia virus integration site 1 (BMI 1); HOXA9; HOXC13; p16 INK4A; RNA interference

Indexed keywords

BMI1 PROTEIN; CYCLIN DEPENDENT KINASE INHIBITOR 2A; MESSENGER RNA; SHORT HAIRPIN RNA; TELOMERASE REVERSE TRANSCRIPTASE; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR HOXA9; TRANSCRIPTION FACTOR HOXB4; TRANSCRIPTION FACTOR HOXC13; UNCLASSIFIED DRUG;

ARTICLE; CELL CYCLE ARREST; CELL CYCLE G1 PHASE; CELL CYCLE S PHASE; DOWN REGULATION; FLOW CYTOMETRY; GENE SILENCING; HELA CELL; HUMAN; HUMAN CELL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RNA INTERFERENCE; UPREGULATION;

BASE SEQUENCE; BLOTTING, WESTERN; CELL CYCLE CHECKPOINTS; CELL SEPARATION; CYCLIN-DEPENDENT KINASE INHIBITOR P16; FLOW CYTOMETRY; GENE EXPRESSION; GENE EXPRESSION REGULATION, NEOPLASTIC; GENE KNOCKDOWN TECHNIQUES; HELA CELLS; HOMEODOMAIN PROTEINS; HUMANS; MOLECULAR SEQUENCE DATA; NEOPLASMS; NUCLEAR PROTEINS; PROTO-ONCOGENE PROTEINS; REAL-TIME POLYMERASE CHAIN REACTION; REPRESSOR PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA INTERFERENCE; RNA, MESSENGER; TRANSFECTION;

EID: 84655170252     PISSN: 13570560     EISSN: 1559131X     Source Type: Journal    
DOI: 10.1007/s12032-010-9634-9     Document Type: Article

References (23)

1. A Kuzmichev T Jenuwein P Tempst D Reinberg 2004 Different EZH2-containing complexes target methylation of histone H1 or nucleosomal histone H3 Mol Cell 14 2 183 193
2. S Schoeftner AK Sengupta S Kubicek K Mechtler L Spahn H Koseki T Jenuwein A Wutz 2006 Recruitment of PRC1 function at the initiation of X inactivation independent of PRC2 and silencing EMBO J 25 13 3110 3122
3. A Kuzmichev R Margueron A Vaquero TS Preissner M Scher A Kirmizis X Ouyang N Brockdorff C Abate-Shen P Farnham D Reinberg 2005 Composition and histone substrates of polycomb repressive group complexes change during cellular differentiation Proc Natl Acad Sci USA 102 6 1859 1864
4. G Buchwald P van der Stoop O Weichenrieder A Perrakis M van Lohuizen TK Sixma 2006 Structure and E3-ligase activity of the Ring-Ring complex of polycomb proteins Bmi1 and Ring1b EMBO J 25 11 2465 2474
5. Z Li R Cao M Wang MP Myers Y Zhang RM Xu 2006 Structure of a Bmi-1-Ring1B polycomb group ubiquitin ligase complex J Biol Chem 281 29 20643 20649
6. JJ Jacobs K Kieboom S Marino RA DePinho M van Lohuizen 1999 The oncogene and polycomb-group gene BMI-1 regulates cell proliferation and senescence through the INK4a locus Nature 397 6715 164 168
7. JJ Jacobs B Scheijen JW Voncken K Kieboom A Berns M van Lohuizen 1999 Bmi-1 collaborates with c-Myc in tumorigenesis by inhibiting c-Myc-induced apoptosis via INK4a/ARF Genes Dev 13 20 2678 2690
8. S Vonlanthen J Heighway HJ Altermatt M Gugger A Kappeler MM Borner M van Lohuizen DC Betticher 2001 The BMI-1 oncoprotein is differentially expressed in non-small cell lung cancer and correlates with INK4A-ARF locus expression Br J Cancer 84 10 372 1376
9. GP Dimri JL Martinez JJ Jacobs P Keblusek K Itahana M Van Lohuizen J Campisi DE Wazer V Band 2002 The Bmi-1 oncogene induces telomerase activity and immortalizes human mammary epithelial cells Cancer Res 62 16 4736 4745
10. NM van der Lugt M Alkema A Berns J Deschamps 1996 The polycomb-group homolog Bmi-1 is a regulator of murine Hox gene expression Mech Dev 58 1-2 153 164
11. S Beà F Tort M Pinyol X Puig L Hernández S Hernández PL Fernandez M van Lohuizen D Colomer E Campo 2001 BMI-1 gene amplification and overexpression in hematological malignancies occur mainly in mantle cell lymphomas Cancer Res 61 6 2409 2412
12. J Lessard G Sauvageau 2003 BMI-1 determines the proliferative capacity of normal and leukaemic stem cells Nature 423 6937 255 260
13. C Leung M Lingbeek O Shakhova J Liu E Tanger P Saremaslani M Van Lohuizen S Marino 2004 BMI-1 is essential for cerebellar development and is overexpressed in human medulloblastomas Nature 428 6980 337 341
14. JH Kim SY Yoon CN Kim JH Joo SK Moon IS Choe YK Choe JW Kim 2004 The BMI-1 oncoprotein is overexpressed in human colorectal cancer and correlates with the reduced p16INK4a/p14ARF proteins Cancer Lett 203 2 217 224
15. T Tuschl 2002 Expanding small RNA interference Nat Biotechnol 20 5 446 448
16. -ΔΔCt method Methods 25 4 402 408
17. AJ Bridge S Pebernard A Ducraux AL Nicoulaz R Iggo 2003 Induction of an interferon response by RNAi vectors in mammalian cells Nat Genet 34 3 263 264
18. D Beuchle G Struhl J Müller 2001 Polycomb group proteins and heritable silencing of Drosophila Hox genes Development 128 6 993 1004
19. A Breiling LP O'Neill D D'Eliseo BM Turner V Orlando 2004 Epigenome changes in active and inactive polycomb-group-controlled regions EMBO Rep 5 10 976 982
20. T Akasaka M van Lohuizen N van der Lugt Y Mizutani-Koseki M Kanno M Taniguchi M Vidal M Alkema A Berns H Koseki 2001 Mice doubly deficient for the polycomb group genes Mel18 and Bmi1 reveal synergy and requirement for maintenance but not initiation of Hox gene expression Development 128 9 1587 1597
21. R Cao Y Tsukada Y Zhang 2005 Role of Bmi-1 and Ring1A in H2A ubiquitylation and HOX gene silencing Mol Cell 20 6 845 854
22. R Cao Y Zhang 2004 SUZ12 is required for both the histone methyltransferase activity and the silencing function of the EED-EZH2 complex Mol Cell 15 1 57 67
23. T Kajiume Y Ninomiya H Ishihara R Kanno M Kanno 2004 Polycomb group gene Mel-18 modulates the self-renewal activity and cell cycle status of hematopoietic stem cells Exp Hematol 32 6 571 578