Over-expression of PKGIα inhibits hypoxia-induced proliferation, Akt activation, and phenotype modulation of human PASMCs: The role of phenotype modulation of PASMCs in pulmonary vascular remodeling

Gene

Volumn 492, Issue 2, 2012, Pages 354-360

  Yi, Bin ^a   Cui, Jian ^a   Ning, Jiao Nin ^a   Wang, Guan Song ^a   Qian, Gui Sheng ^a   Lu, Kai Zhi ^a  

^a THIRD MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Human pulmonary artery smooth muscle cells; Hypoxia; Phenotype modulation; Proliferation; Protein kinase G; Pulmonary vascular remodeling

Indexed keywords

ADENOVIRUS VECTOR; ALPHA SMOOTH MUSCLE ACTIN; CALPONIN; CYCLIC GMP DEPENDENT PROTEIN KINASE; MYOSIN HEAVY CHAIN; PROTEIN KINASE B; THYMIDINE;

ARTICLE; CELL PROLIFERATION; CONTROLLED STUDY; DOWN REGULATION; ENZYME ACTIVATION; FLOW CYTOMETRY; GENE OVEREXPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HYPOXIA; LUNG BLOOD VESSEL; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PULMONARY ARTERY SMOOTH MUSCLE CELLS; SMOOTH MUSCLE FIBER; TISSUE REGENERATION; UPREGULATION;

ANOXIA; CELL HYPOXIA; CELL PROLIFERATION; CELLS, CULTURED; CYCLIC GMP-DEPENDENT PROTEIN KINASES; HUMANS; MUSCLE, SMOOTH, VASCULAR; PHENOTYPE; PROTO-ONCOGENE PROTEINS C-AKT; PULMONARY ARTERY; TRANSFECTION; UP-REGULATION;

ADENOVIRIDAE;

EID: 84655162225     PISSN: 03781119     EISSN: 18790038     Source Type: Journal    
DOI: 10.1016/j.gene.2011.11.010     Document Type: Article

References (28)

1. Aoshima D., Murata T., Hori M., Ozaki H. Time-dependent phenotypic and contractile changes of pulmonary artery in chronic hypoxia-induced pulmonary hypertension. J. Pharmacol. Sci. 2009, 110:182-190.
2. Bai L., et al. SOCS3 was induced by hypoxia and suppressed STAT3 phosphorylation in pulmonary arterial smooth muscle cells. Respir. Physiol. Neurobiol. 2006, 152:83-91.
3. Barman S.A., Zhu S., Han G., White R.E. cAMP activates BKCa channels in pulmonary arterial smooth muscle via cGMP-dependent protein kinase. Am. J. Physiol. Lung Cell. Mol. Physiol. 2003, 284:L1004-L1011.
4. Boerth N.J., Dey N.B., Cornwell T.L., Lincoln T.M. Cyclic GMP-dependent protein kinase regulates vascular smooth muscle cell phenotype. J. Vasc. Res. 1997, 34:245-259.
5. Dostmann W.R., Taylor M.S., Nickl C.K., Brayden J.E., Frank R., Tegge W.J. Highly specific, membrane- permeant peptide blockers of cGMP-dependent protein kinase Ialpha inhibit NO-induced cerebral dilation. Proc. Natl. Acad. Sci. U. S. A. 2000, 97:14772-14777.
6. Fager G., Hansson G.K., Gown A.M., Larson D.M., Skalli O., Bondjers G. Human arterial smooth muscle cells in culture: inverse relationship between proliferation and expression of contractile proteins. In Vitro Cell. Dev. Biol. 1989, 25:511-520.
7. Fukumoto Y., Tawara S., Shimokawa H. Recent progress in the treatment of pulmonary arterial hypertension:expectation for rho-kinase inhibitors. Tohoku J. Exp. Med. 2007, 211:309-320.
8. Goncharova E.A., et al. PI3K is required for proliferation and migration of human pulmonary vascular smooth muscle cells. Am. J. Physiol. Lung Cell. Mol. Physiol. 2002, 283:L354-L363.
9. Gudi T., Chen J.C., Gasteel D.E., Seasholtz T.M., Boss G.R., Pilz R.B. cGMP-dependent protein kinase inhibits serum-response element-dependent transcription by inhibiting rho activation and functions. J. Biol. Chem. 2002, 277:37382-37393.
10. Humbert M., Sitbon O., Simonneau G. Treatment of pulmonary arterial hypertension. N. Engl. J. Med. 2004, 351:1425-1436.
11. Kouri F.M., et al. Plasminogen activator inhibitor type 1 inhibits smooth muscle cell proliferation in pulmonary arterial hypertension. Int. J. Biochem. Cell Biol. 2008, 40:1872-1882.
12. Lincoln T.M., Wu X., Sellak H., Dey N., Choi C.S. Regulation of vascular smooth muscle cell phenotype by cyclic GMP and cyclic GMP-depend protein kinase. Front. Biosci. 2006, 11:356-357.
13. Luo C.X., et al. Suppression of Akt1 phosphorylation by adenoviral transfer of the PTEN gene inhibits hypoxia-induced proliferation of rat pulmonary arterial smooth muscle cells. Biochem. Biophys. Res. Commun. 2010, 397:486-492.
14. Mahoney W.M., Schwartz S.M. Defining smooth muscle cells and smooth muscle injury. J. Clin. Invest. 2005, 115:212-224.
15. Morrell N.W., et al. Cellular and molecular basis of pulmonary arterial hypertension. J. Am. Coll. Cardiol. 2009, 54:S20-S31.
16. Moussallem M.D., Olenych S.G., Scott S.L., Keller T.C., Schlenoff J.B. Smooth muscle cell phenotype modulation and contraction on native and cross-linked polyelectrolyte multilayers. Biomacromolecules 2009, 10:3062-3068.
17. Owens G.K., Kumar M.S., Wamhoff B.R. Molecular regulation of vascular smooth muscle cell differentiation in development and disease. Physiol. Rev. 2004, 84:767-801.
18. Pak O., Aldashev A., Welsh D., Peacock A. The effects of hypoxia on the cells of the pulmonary vasculature. Eur. Respir. J. 2007, 30:364-372.
19. Rhodes C.J., Davidson A., Gibbs J.S., Wharton J., Wilkins M.R. Therapeutic targets in pulmonary arterial hypertension. Pharmacol. Ther. 2009, 121:69-88.
20. Simon A.R., Takahashi S., Severgnini M., Fanburg B.L., Cochran B.H. Role of the JAK-STAT pathway in PDGF-stimulated proliferation of human airway smooth muscle cells. Am. J. Physiol. Lung Cell. Mol. Physiol. 2002, 282:L1296-L1304.
21. St.Hilaire R.C., Kadowitz P.J., Jeter J.R. Adenoviral transfer of vasoactive intestinal peptide (VIP) gene inhibits rat aortic and pulmonary artery smooth muscle cell proliferation. Peptides 2009, 30:2323-2329.
22. Stegemann J.P., Dey N.B., Lincoln T.M., Nerem R.M. Genetic modification of smooth muscle cells to control phenotype and function in vascular tissue engineering. Tissue Eng. 2004, 10:189-199.
23. Stenmark K.R., Fagan K.A., Frid M.G. Hypoxia-induced pulmonary vascular remodeling: cellular and molecular mechanisms. Circ. Res. 2006, 99:675-691.
24. Wang G.S., Qian G.S., Zhou D.S., Zhao J.Q. JAK-STAT signaling pathway in pulmonary arterial smooth muscle cells is activated by hypoxia. Cell Biol. Int. 2005, 29:598-603.
25. Yi B., Lu J.Y., Qian G.S. Changes of cell phenotype and PKGIα expression in pulmonary arterial smooth muscle cells induced by hypoxia. Mil. Med. J. South China 2008, 22:29-31.
26. Yi B., Lu J.Y., Bai L., Wang G.S., Qian G.S. Construction and identification of reccombinant adenovirus containing wild-type human PKGIα gene. Acad. J. Sec. Mil. Med. Univ. 2009, 30:69-72.
27. Zhou L., Hershenson M.B. Mitogenic signaling pathways in airway smooth muscle. Respir. Physiol. Neurobiol. 2003, 137(2-3):295-308.
28. Zhou W., Negash S., Liu J., Raj J.U. Modulation of pulmonary vascular smooth muscle cell phenotype in hypoxia: role of cGMP-dependent protein kinase and myocardin. Am. J. Physiol. Lung Cell. Mol. Physiol. 2009, 296:L780-L789.