Optimal time for passaging neurospheres based on primary neural stem cell cultures

Cytotechnology

Volumn 63, Issue 6, 2011, Pages 621-631

  Xiong, Fangling ^a   Gao, Huasong ^b   Zhen, Yan ^a   Chen, Xue ^a   Lin, Weiwei ^a   Shen, Jianhong ^a   Yan, Yaohua ^a   Wang, Xiaodong ^a   Liu, Mei ^a   Gao, Yilu ^a  

^a NANTONG UNIVERSITY   (China)

^b FUDAN UNIVERSITY   (China)

Author keywords

Cell passage; Neural stem cells; Neurosphere; Optimal time; Primary culture

Indexed keywords

CYCLIN D1; CYCLIN DEPENDENT KINASE INHIBITOR 2A;

ARTICLE; CELL ACTIVITY; CELL CULTURE; CELL CYCLE G0 PHASE; CELL CYCLE G1 PHASE; CELL METABOLISM; CELL PROLIFERATION; CELL REGENERATION; CELL STRUCTURE; CENTRAL NERVOUS SYSTEM DISEASE; NERVE CELL DIFFERENTIATION; NEURAL STEM CELL; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION;

EID: 84655161566     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-011-9379-0     Document Type: Article

References (32)

1. J Bartek J Lukas 2001 Pathways governing G1/S transition and their response to DNA damage FEBS Lett 490 117 122 10.1016/S0014-5793(01)02114-7 10.1016/S0014-5793(01)02114-7 1:CAS:528:DC%2BD3MXht1Ortrs%3D (Pubitemid 32171930)
2. AE Bassiouny MM Nosseir MK Zoheiry NA Ameen AM Abdel-Hadi IM Ibrahim S Zada AH El-Deen NE El-Bassiouni 2010 Differential expression of cell cycle regulators in HCV-infection and related hepatocellular carcinoma World J Hepatol 2 32 41 10.4254/wjh.v2.i1.32
3. A Bez E Corsini D Curti M Biggiogera A Colombo RF Nicosia SF Pagano EA Parati 2003 Neurosphere and neurosphere-forming cells: morphological and ultrastructural characterization Brain Res 993 18 29 10.1016/j.brainres.2003.08. 061 10.1016/j.brainres.2003.08.061 1:CAS:528:DC%2BD3sXpt1elt7w%3D (Pubitemid 37464579)
4. KE Bornfeldt 2003 The cyclin-dependent kinase pathway moves forward Circ Res 92 345 347 10.1161/01.RES.0000061765.06145.10 10.1161/01.RES.0000061765. 06145.10 1:CAS:528:DC%2BD3sXjtlWis7s%3D (Pubitemid 36298192)
5. GJ Brewer JR Torricelli 2007 Isolation and culture of adult neurons and neurospheres Nat Protoc 2 1490 1498 10.1038/nprot.2007.207 10.1038/nprot.2007. 207 1:CAS:528:DC%2BD2sXhtFagsLnJ (Pubitemid 46952327)
6. Y Chen V Balasubramaniyan J Peng EC Hurlock M Tallquist J Li QR Lu 2007 Isolation and culture of rat and mouse oligodendrocyte precursor cells Nat Protoc 2 1044 1051 10.1038/nprot.2007.149 10.1038/nprot.2007.149 1:CAS:528:DC%2BD2sXhtFGnurzL (Pubitemid 46901285)
7. SK Dhara SL Stice 2008 Neural differentiation of human embryonic stem cells J Cell Biochem 105 633 640 10.1002/jcb.21891 10.1002/jcb.21891 1:CAS:528:DC%2BD1cXht1Kqu7fK
8. R Favaro M Valotta AL Ferri E Latorre J Mariani C Giachino C Lancini V Tosetti S Ottolenghi V Taylor SK Nicolis 2009 Hippocampal development and neural stem cell maintenance require Sox2-dependent regulation of Shh Nat Neurosci 12 1248 1256 10.1038/nn.2397 10.1038/nn.2397 1:CAS:528:DC%2BD1MXhtV2gsbnN
9. S Goldman 2005 Stem and progenitor cell-based therapy of the human central nervous system Nat Biotechnol 23 862 871 10.1038/nbt1119 10.1038/nbt1119 1:CAS:528:DC%2BD2MXlvFegs7Y%3D (Pubitemid 43093130)
10. JB Kim H Zaehres G Wu L Gentile K Ko V Sebastiano MJ Arauzo-Bravo D Ruau DW Han M Zenke HR Scholer 2008 Pluripotent stem cells induced from adult neural stem cells by reprogramming with two factors Nature 454 646 650 10.1038/nature07061 10.1038/nature07061 1:CAS:528:DC%2BD1cXptFOksrY%3D
11. JB Kim V Sebastiano G Wu MJ Arauzo-Bravo P Sasse L Gentile K Ko D Ruau M Ehrich D van den Boom J Meyer K Hubner C Bernemann C Ortmeier M Zenke BK Fleischmann H Zaehres HR Scholer 2009 Oct4-induced pluripotency in adult neural stem cells Cell 136 411 419 10.1016/j.cell.2009.01.023 10.1016/j.cell.2009.01. 023 1:CAS:528:DC%2BD1MXltFSnsr4%3D
12. A Krishan 1975 Rapid flow cytofluorometric analysis of mammalian cell cycle by propidium iodide staining J Cell Biol 66 188 193 10.1083/jcb.66.1.188 10.1083/jcb.66.1.188 1:STN:280:DyaE2M7ovFKkug%3D%3D
13. C Lange WB Huttner F Calegari 2009 Cdk4/cyclinD1 overexpression in neural stem cells shortens G1, delays neurogenesis, and promotes the generation and expansion of basal progenitors Cell Stem Cell 5 320 331 10.1016/j.stem.2009.05. 026 10.1016/j.stem.2009.05.026 1:CAS:528:DC%2BD1MXhsVCiurbE
14. XG Li ZY Yang AF Zhang 2009 The effect of neurotrophin-3/chitosan carriers on the proliferation and differentiation of neural stem cells Biomaterials 30 4978 4985 10.1016/j.biomaterials.2009.05.047 10.1016/j.biomaterials.2009.05.047 1:CAS:528:DC%2BD1MXpt1Cisbs%3D
15. MVT Lobo FJM Alonso C Redondo MA Lopez-Toledano E Caso AS Herranz CL Paino D Reimers E Bazan 2002 Cellular characterization of epidermal growth factor-expanded free-floating neurospheres J Histochem Cytochem 51 89 103 10.1177/002215540305100111 10.1177/002215540305100111
16. SW Lowe CJ Sherr 2003 Tumor suppression by Ink4a-Arf: progress and puzzles Curr Opin Genet Dev 13 77 83 10.1016/S0959-437X(02)00013-8 10.1016/S0959-437X(02)00013-8 1:CAS:528:DC%2BD3sXmsFCisQ%3D%3D (Pubitemid 36143931)
17. AV Molofsky R Pardal T Iwashita IK Park MF Clarke SJ Morrison 2003 Bmi-1 dependence distinguishes neural stem cell self-renewal from progenitor proliferation Nature 425 962 967 10.1038/nature02060 10.1038/nature02060 1:CAS:528:DC%2BD3sXosVOht7w%3D (Pubitemid 37376927)
18. AV Molofsky SG Slutsky NM Joseph S He R Pardal J Krishnamurthy NE Sharpless SJ Morrison 2006 Increasing p16INK4a expression decreases forebrain progenitors and neurogenesis during ageing Nature 443 448 452 10.1038/nature05091 10.1038/nature05091 1:CAS:528:DC%2BD28XhtVSns7bL (Pubitemid 44477280)
19. V Ourednik J Ourednik YF Xu Y Zhang WP Lynch EY Snyder M Schachner 2009 Cross-talk between stem cells and the dysfunctional brain is facilitated by manipulating the niche: evidence from an adhesion molecule Stem Cells 27 2846 2856 10.1002/stem.227 10.1002/stem.227 1:CAS:528:DC%2BC3cXlsFyrsg%3D%3D
20. L Prestoz JB Relvas K Hopkins S Patel P Sowinski J Price C ffrench-Constant 2001 Association between integrin-dependent migration capacity of neural stem cells in vitro and anatomical repair following transplantation Mol Cell Neurosci 18 473 484 10.1006/mcne.2001.1037 10.1006/mcne.2001.1037 1:CAS:528:DC%2BD3MXosFGgtrY%3D (Pubitemid 34008388)
21. BA Reynolds S Weiss 1992 Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system Science 255 1701 1710 10.1126/science.1553558 10.1126/science.1553558
22. BA Reynolds S Weiss 1996 Clonal and population analyses demonstrate that an EGF-responsive mammalian embryonic CNS precursor is a stem cell Dev Biol 175 1 13 10.1006/dbio.1996.0090 10.1006/dbio.1996.0090 1:CAS:528:DyaK28Xitlynt7Y%3D (Pubitemid 26150042)
23. P Salomoni F Calegari 2010 Cell cycle control of mammalian neural stem cells: putting a speed limit on G1 Trends Cell Biol 205 233 243 10.1016/j.tcb.2010.01.006 10.1016/j.tcb.2010.01.006
24. CJ Sherr 2001 The INK4a/ARF network in tumour suppression Nat Rev Mol Cell Biol 2 731 737 10.1038/35096061 10.1038/35096061 1:CAS:528: DC%2BD3MXnvVajs7Y%3D (Pubitemid 33679835)
25. P Sicinski JL Donaher SB Parker T Li A Fazeli H Gardner SZ Haslam RT Bronson SJ Elledge RA Weinberg 1995 Cyclin D1 provides a link between development and oncogenesis in the retina and breast Cell 82 621 630 10.1016/0092-8674(95)90034-9 10.1016/0092-8674(95)90034-9 1:CAS:528: DyaK2MXnvVWmtLc%3D
26. M Sugimoto T Nakamura N Ohtani L Hampson IN Hampson A Shimamoto Y Furuichi K Okumura S Niwa Y Taya E Hara 1999 Regulation of CDK4 activity by a novel CDK4-binding protein, p34(SEI-1) Genes Dev 13 3027 3033 10.1101/gad.13.22.3027 1:CAS:528:DyaK1MXnvVCltbs%3D
27. Y Sun S Pollard L Conti M Toselli G Biella G Parkin L Willatt A Falk E Cattaneo A Smith 2008 Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture Mol Cell Neurosci 38 245 258 10.1016/j.mcn.2008.02.014 10.1016/j.mcn.2008.02.014 1:CAS:528: DC%2BD1cXntlCqtL0%3D
28. ON Suslov VG Kukekov TN Ignatova DA Steindler 2002 Neural stem cell heterogeneity demonstrated by molecular phenotyping of clonal neurospheres Proc Natl Acad Sci USA 99 14506 14511 10.1073/pnas.212525299 10.1073/pnas.212525299 1:CAS:528:DC%2BD38XosF2hsL4%3D (Pubitemid 35257701)
29. CN Svendsen AG Smith 1999 New prospects for human stem cell therapy in the nervous system Trends Neurosci 22 357 364 10.1016/S0166-2236(99)01428-9 10.1016/S0166-2236(99)01428-9 1:CAS:528:DyaK1MXltVSgsbo%3D (Pubitemid 29339249)
30. V Tropepe M Sibilia BG Ciruna J Rossant EF Wagner D van der Kooy 1999 Distinct neural stem cells proliferate in response to EGF and FGF in the developing mouse telencephalon Dev Biol 208 166 188 10.1006/dbio.1998.9192 10.1006/dbio.1998.9192 1:CAS:528:DyaK1MXhslejtLc%3D (Pubitemid 29149456)
31. V Vukicevic A Jauch TC Dinger L Gebauer V Hornich SR Bornstein M Ehrhart-Bornstein AM Müller 2010 Genetic instability and diminished differentiation capacity in long-term cultured mouse neurosphere cells Mech Ageing Dev 131 124 132 10.1016/j.mad.2010.01.001 10.1016/j.mad.2010.01.001 1:CAS:528:DC%2BC3cXitVKjtrk%3D
32. S Ye ZP Su J Zhang X Qian QC Zhuge YJ Zeng 2008 Differential centrifugation in culture and differentiation of rat neural stem cells Cell Mol Neurobiol 28 511 517 10.1007/s10571-007-9194-5 10.1007/s10571-007-9194-5 (Pubitemid 351624507)