Purification of native bone marrow megakaryocytes for studies of gene expression

Methods in Molecular Biology

Volumn 788, Issue , 2012, Pages 259-273

  Tolhurst, Gwen ^a   Carter, Richard N ^a   Miller, Nigel ^a   Mahaut Smith, Martyn P ^b  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b UNIVERSITY OF LEICESTER   (United Kingdom)

Author keywords

Ion channel; Megakaryocyte RNA; PCR; Purification; Single cell PCR

Indexed keywords

MESSENGER RNA;

ANIMAL; ARTICLE; BONE MARROW; C57BL MOUSE; CELL SEPARATION; CYTOLOGY; GENE EXPRESSION PROFILING; GENETICS; ISOLATION AND PURIFICATION; MEGAKARYOCYTE; METABOLISM; METHODOLOGY; MOUSE; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALS; BONE MARROW; CELL SEPARATION; GENE EXPRESSION PROFILING; MEGAKARYOCYTES; MICE; MICE, INBRED C57BL; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER;

MURINAE;

EID: 84555191621     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-307-3_18     Document Type: Article

References (17)

1. Wright, J. H. (1910) The histogenesis of the blood platelets Journal of Morphology 21 , 263-278.
2. Wright, J. H. (1906) The origin and nature of the blood plates Boston Medical and Surgical Journal 154 , 643-645.
3. Italiano, J. E., Jr. and Shivdasani, R. A. (2003) Megakaryocytes and beyond: the birth of platelets J Thromb. Haemost. 1 , 1174-1182.
4. Shattil, S. J. and Leavitt, A. D. (2001) All in the family: primary megakaryocytes for studies of platelet a IIb b 3 signaling Thromb. Haemost. 86 , 259-265. (Pubitemid 32663956)
5. Tolhurst, G., Vial, C., Leon, C., Gachet, C., Evans, R. J., and Mahaut-Smith, M. P. (2005) Interplay between P2Y 1 , P2Y 12 , and P2X 1 receptors in the activation of megakaryocyte cation influx currents by ADP: evidence that the primary megakaryocyte represents a fully functional model of platelet P2 receptor signaling Blood 106 , 1644-1651. (Pubitemid 41208577)
6. Mason, M. J. and Mahaut-Smith, M. P. (2004) Measurement and manipulation of intracellular Ca 2+ in single platelets and megakaryocytes Methods Mol. Biol. 273 , 251-276.
7. Mahaut-Smith, M. P. (2004) Patch-clamp recordings of electrophysiological events in the platelet and megakaryocyte Methods Mol. Biol. 273 , 277-300.
8. Mason, M. J. and Mahaut-Smith, M. P. (2001) Voltage-dependent Ca 2+ release in rat megakaryocytes requires functional IP 3 receptors J Physiol 533 , 175-183. (Pubitemid 32499370)
9. Tertyshnikova, S. and Fein, A. (1998) Inhibition of inositol 1,4,5-trisphosphate-induced Ca 2+ release by cAMP-dependent protein kinase in a living cell Proc Natl Acad Sci USA 95 , 1613-1617.
10. Monyer, H. and Jonas, P. (2010) in Single Channel Recording (Sakmann-B and Neher-H, Eds.).
11. Jonas, P., Racca, C., Sakmann, B., Seeburg, P. H., and Monyer, H. (1994) Differences in Ca 2+ permeability of AMPA-type glutamate receptor channels in neocortical neurons caused by differential GluR-B subunit expression Neuron 12 , 1281-1289.
12. Lambolez, B., Audinat, E., Bochet, P., Crepel, F., and Rossier, J. (1992) AMPA receptor subunits expressed by single Purkinje cells Neuron 9 , 247-258.
13. de Preter, K., Vandesompele, J., Heimann, P., Kockx, M. M., Van Gele, M., Hoebeeck, J., De Smet, E., Demarche, M., Laureys, G., Van Roy, N., De Paepe, A., and Speleman, F. (2003) Application of laser capture microdissection in genetic analysis of neuroblastoma and neuroblastoma precursor cells Cancer Lett. 197 , 53-61. (Pubitemid 36859990)
14. Iyer, E. P. and Cox, D. N. (2010) Laser capture microdissection of Drosophila peripheral neurons J. Vis. Exp.
15. Carter, R. N., Tolhurst, G., Walmsley, G., Vizuete-Forster, M., Miller, N., and Mahaut-Smith, M. P. (2006) Molecular and electrophysiological characterization of transient receptor potential ion channels in the primary murine megakaryocyte J. Physiol 576 , 151-162. (Pubitemid 44426227)
16. Raslova, H., Roy, L., Vourc'h, C., Le Couedic, J. P., Brison, O., Metivier, D., Feunteun, J., Kroemer, G., Debili, N., and Vainchenker, W. (2003) Megakaryocyte polyploidization is associated with a functional gene amplification Blood 101 , 541-544. (Pubitemid 36077575)
17. Leven, R. M. (2004) Isolation of primary megakaryocytes and studies of proplatelet formation Methods Mol. Biol. 272 , 281-292.