Expanding human T regulatory cells with the mTOR-inhibitor rapamycin

Methods in Molecular Biology

Volumn 821, Issue , 2012, Pages 279-293

  Battaglia, Manuela ^a   Stabilini, Angela ^b   Tresoldi, Eleonora ^a  

^a SAN RAFFAELE SCIENTIFIC INSTITUTE   (Italy)

^b SAN RAFFAELE HOSPITAL   (Italy)

Author keywords

Immunological tolerance; Rapamycin; Regulatory T cells

Indexed keywords

FORKHEAD TRANSCRIPTION FACTOR; FOXP3 PROTEIN, HUMAN; MTOR PROTEIN, HUMAN; RAPAMYCIN; TARGET OF RAPAMYCIN KINASE;

ANIMAL; ARTICLE; CELL PROLIFERATION; CELL SEPARATION; CULTURE TECHNIQUE; CYTOLOGY; DRUG ANTAGONISM; DRUG EFFECT; FLOW CYTOMETRY; GENETICS; HUMAN; IMMUNOLOGICAL TOLERANCE; METABOLISM; METHODOLOGY; MOUSE; REGULATORY T LYMPHOCYTE;

ANIMALS; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CELL SEPARATION; FLOW CYTOMETRY; FORKHEAD TRANSCRIPTION FACTORS; HUMANS; IMMUNE TOLERANCE; MICE; SIROLIMUS; T-LYMPHOCYTES, REGULATORY; TOR SERINE-THREONINE KINASES;

MURINAE; MUS;

EID: 84555187731     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-430-8_17     Document Type: Article

References (8)

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2. Wing, K., and S. Sakaguchi (2010) Regulatory T cells exert checks and balances on self tolerance and autoimmunity. Nat Immunol 11, 7-13.
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4. Roncarolo, M. G., and M. Battaglia (2007) Regulatory T-cell immunotherapy for tolerance to self antigens and alloantigens in humans. Nat Rev Immunol 7, 585-598. (Pubitemid 47123554)
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6. Battaglia, M., A. Stabilini, B. Migliavacca, J. Horejs-Hoeck, T. Kaupper, and M. G. Roncarolo (2006) Rapamycin promotes expansion of functional CD4 + CD25 + FOXP3+ regulatory T cells of both healthy subjects and type 1 diabetic patients. J Immunol 177, 8338-8347. (Pubitemid 44893795)
7. Foxp3-mediated induction of pim 2 allows human T regulatory cells to preferentially expand in rapamycin. Basu S, Golovina T, Mikheeva T, June CH, Riley JL. J Immunol. 2008 May 1;180(9):5794-8.
8. Stability of human rapamycin-expanded CD4+CD25+ T regulatory cells. Tresoldi E, Dell'albani I, Stabilini A, Jofra T, Valle A, Gagliani N, Bondanza A, Roncarolo MG, Battaglia M. Haematologica. 2011 Sep; 96(9):1357-65.