Transfection by Electroporation

Current Protocols in Neuroscience

Volumn , Issue SUPPL.57, 2011, Pages

  Potter, Huntington ^a   Heller, Richard ^b  

^a UNIVERSITY OF SOUTH FLORIDA   (United States)

^b OLD DOMINION UNIVERSITY   (United States)

Author keywords

DNA vaccine; Gene expression; Gene regulation; Gene therapy; Introduction of DNA into cells; Molecular biology; Transcription and translation

Indexed keywords

DNA;

ARTICLE; CANCER GENE THERAPY; DNA IMMUNIZATION; ELECTROPORATION; EMBRYONIC STEM CELL; GENE EXPRESSION; GENETIC TRANSFECTION; IN VIVO GENE TRANSFER; MAMMAL CELL; NONHUMAN; NONVIRAL GENE THERAPY; PRIORITY JOURNAL; PROTOPLAST;

EID: 84255170527     PISSN: 19348584     EISSN: 19348576     Source Type: Journal    
DOI: 10.1002/0471142301.nsa01es57     Document Type: Article

References (36)

1. Abdulhaqq, S.A. and Weiner, D.B. 2008. DNA vaccines: Developing new strategies to enhance immune responses. Immunol. Res. 42:219-232.
2. Andre, F. and Mir, L.M. 2004. DNAelectrotransfer: Its principles and an updated review of its therapeutic applications. Gene Ther. 11:S33-S42.
3. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. 2001. Phenol/SDS method for plant RNApreparation. Curr. Protoc. Mol. Biol. 9:4.3.1-4.3.4.
4. Bloch, K.D. and Grossmann, B. 2001. Digestion of DNA with restriction endonucleases. Curr. Protoc. Mol. Biol. 31:3.1.1-3.1.21.
5. Bodles-Brakhop, A.M., Heller, R., and Draghia-Akli, R. 2009. Electroporation for the delivery of DNA-based vaccines and immunotherapeutics: Current clinical developments. Mol. Ther. 17:585-592.
6. Bronson, S.K. and Smithies, O. 1994. Altering mice by homologous recombination using embryonic stem cells. J. Biol. Chem. 269:27155-27158.
7. Chang, D.C. 1989. Cell poration and cell fusion using an oscillating electric field. Biophys. J. 56:641-652.
8. Chu, G., Hayakawa, H., and Berg, P. 1987. Electroporation for the efficient transfection of mammalian cells with DNA. Nucleic Acids Res. 15:1311-1326.
9. Elbing, K. and Brent, R. 2002. Growth in liquid media. Curr. Protoc. Mol. Biol. 59:1.2.1-1.2.2.
10. Fromm, M., Taylor, L.P., and Walbot, V. 1985. Expression of genes transferred into monocot and dicot plant cells by electroporation. Proc. Natl. Acad. Sci. U.S.A. 82:5824-5828.
11. Fuller, S.A., Takahashi, M., and Hurrell, J.G. 2001a. Preparation of myeloma cells. Curr. Protoc. Mol. Biol. 18:11.5.1-11.5.3.
12. Fuller, S.A., Takahashi,M. and Hurrell, J.G. 2001b. Cloning of hybridoma cell lines by limiting dilution. Curr. Protoc. Mol. Biol. 1:11.8.1-11.8.2.
13. Glasspool-Malone, J., Somiari, S., Drabick, J., and Malone, R. 2000. Efficient nonviral cutaneous transfection. Mol. Ther. 2:140-146.
14. Gothelf, A., Mir, L.M., and Gehl, J. 2003. Electrochemotherapy: Results of cancer treatment using enhanced delivery of bleomycin by electroporation. Cancer Treat. Rev. 29:371-387.
15. Heilig, J., Elbing, K.L., and Brent, R. 2001. Largescale preparation of plasmidDNA. Curr. Protoc. Mol. Biol. 41:1.7.1-1.7.16.
16. Heller, L.C. and Heller, R. 2006. In vivo electroporation for gene therapy. Hum. Gene Ther. 17:890-897.
17. Heller, L.C., Jaroszeski, M.J., Coppola, D., Mc-Crae, A.N., Hickey, J., and Heller, R. 2006. Optimization of cutaneous electrically mediated plasmid DNA delivery using a novel electrode. Gene Ther. 14:275-280.
18. Heller, R., Jaroszeski, M., Atkin, A., Moradpour, D., Gilbert, R.,Wands, J., and Nicolau, C. 1996. In vivo gene electroinjection and expression in rat liver fed. FEBS Lett. 389:225-228.
19. Hirao, L.A., Wu, L., Khan, A.S., Satishchandran, A.,Draghia-Akli, R., andWeiner, D.B. 2008. Intradermal/ subcutaneous immunization by electroporation improves plasmid vaccine delivery and potency in pigs and rhesus macaques. Vaccine 26:440-448.
20. Joyner, A.L. 2000. Gene Targeting. Oxford University Press, Oxford, United Kingdom.
21. Kingston, R.E., Sheen, J., andMoore, D. 2001. Isotopic assays for reporter gene activity. Curr. Protoc. Mol. Biol. 63:9.7.1-9.7.11.
22. Lucas, M.L. and Heller, R. 2001. Immunomodulation by electrically enhanced delivery of a plasmid encoding IL-12 to murine skeletal muscle. Mol. Ther. 3:47-53.
23. Mir, L.M., Bureau, M.F., Gehl, J., Rangara, R., Rouy, D., Caillaud, J-M., Delaere, P., Branellec, D., Schwartz, B., and Scherman, D. 1999. Highefficiency gene transfer into skeletal musclemediated by electric pulses. Proc. Natl. Acad. Sci. U.S.A. 96:4262-4267.
24. Moore, D. and Dowhan, D. 2002. Purification and concentration of DNA from aqueous solutions. Curr. Protoc. Mol. Biol. 59:2.1.1-2.1.10.
25. Neumann, E., Schaefer-Ridder, M., Wang, Y., and Hofschneider, P.H. 1982. Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO J. 1:841-845.
26. Nishi, T., Yoshizato, K., Yamashiro, S., Takeshima, H., Sato, K., Hamada, K., Kitamura, I., Yoshimura, T., Saya, H., Kuratsu, J., and Ushio, Y. 1996. High-efficiency in vivo gene transfer using intraarterial plasmid DNA injection following in vivo electroporation. Cancer Res. 56:1050-1055.
27. Ou-Lee, T.M., Turgeon, R., and Wu, R. 1986. Uptake and expression of a foreign gene linked to either a plant virus or Drosophila promoter in protoplasts of rice, wheat and sorghum. Proc. Natl. Acad. Sci. U.S.A. 83:6815-6819.
28. Potter, H. 1988. Electroporation in biology: Methods, applications, and instrumentation. Anal. Biochem. 174:361-373.
29. Potter, H., Weir, L., and Leder, P. 1984. Enhancerdependent expression of human κ immunoglobulin genes introduced into mouse pre-B lymphocytes by electroporation. Proc. Natl. Acad. Sci. U.S.A. 81:7161-7165.
30. Rhodes, C.A., Pierce, D.A.,Mettler, I.J.,Mascarenhas, D., and Detmar, J.J. 1988. Genetically transformed maize plants from protoplasts. Science 240:204-207.
31. Roos, A.K., Moreno, S., Leder, C., Pavlenko, M., King, A., and Pisa, P. 2006. Enhancement of cellular immune response to a prostate cancer DNA vaccine by intradermal electroporation. Mol. Ther. 13:320-327.
32. Saunders, J.A., Matthews, B.F., and Miller, P.D. 1989. Plant gene transfer using electrofusion and electroporation. In Electroporation and Electrofusion in Cell Biology (E., Neumann, A.E., Sowers, and C.A., Jordan, eds.) pp. 343-354. Plenum, New York.
33. Sheen, J. 1990. Metabolic repression of transcription in higher plants. Plant Cell 2:1027-1038.
34. Titomirov, A.V., Sukharev, S., and Kistanova, E. 1991. In vivo electroporation and stable transformation of skin cells of newbornmice by plasmid DNA. Biochim. Biophys. Acta 1088:131-134.
35. Trollet, C., Bloquel, C., Scherman, D., and Bigey, P. 2006. Electrotransfer into skeletal muscle for protein expression. Curr. Gene Ther. 6:561-578.
36. Wong, T.K. and Neumann, E. 1982. Electric field mediated gene transfer. Biochem. Biophys. Res. Commun. 107:584-587.