Nucleofection-based gene targeting in human pre-B cells

Gene

Volumn 492, Issue 1, 2012, Pages 305-308

  Kurosawa, Aya ^a   Saito, Shinta ^a   Mori, Mikako ^a   Adachi, Noritaka ^a  

^a YOKOHAMA CITY UNIVERSITY   (Japan)

Author keywords

Gene targeting; Human cell; Nucleofection; Transfection

Indexed keywords

GENOMIC DNA; GREEN FLUORESCENT PROTEIN;

ARTICLE; B LYMPHOCYTE; CELL SURVIVAL; DNA TRANSFECTION; ELECTROPORATION; GENE TARGETING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; LYMPHOID PROGENITOR CELL; NONVIRAL GENE DELIVERY SYSTEM; NUCLEOFECTION; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

CELL LINE, TUMOR; CELL NUCLEUS; CELL SURVIVAL; ELECTROPORATION; GENE TARGETING; HUMANS; PRECURSOR CELLS, B-LYMPHOID; TRANSFECTION;

MAMMALIA;

EID: 84155180743     PISSN: 03781119     EISSN: 18790038     Source Type: Journal    
DOI: 10.1016/j.gene.2011.11.003     Document Type: Article

References (13)

1. Adachi N., Kurosawa A., Koyama H. Highly proficient gene targeting by homologous recombination in the human pre-B cell line Nalm-6. Methods Mol. Biol. 2008, 435:17-29.
2. Adachi N., Nishijima H., Shibahara K. Gene targeting using the human Nalm-6 pre-B cell line. Biosci. Trends 2008, 2:169-180.
3. Bier M., Hammer S.M., Canaday D.J., Lee R.C. Kinetics of sealing for transient electropores in isolated mammalian skeletal muscle cells. Bioelectromagnetics 1999, 20:194-201.
4. Capecchi M.R. Altering the genome by homologous recombination. Science 1989, 244:1288-1292.
5. Golzio M., Mora M.P., Raynaud C., Delteil C., Teissié J., Rols M.P. Control by osmotic pressure of voltage-induced permeabilization and gene transfer in mammalian cells. Biophys. J. 1998, 74:3015-3022.
6. Gresch O., et al. New non-viral method for gene transfer into primary cells. Methods 2004, 33:151-163.
7. Iiizumi S., et al. Simple one-week method to construct gene-targeting vectors: application to production of human knockout cell lines. Biotechniques 2006, 41:311-316.
8. Iiizumi S., et al. Impact of non-homologous end-joining deficiency on random and targeted DNA integration: implications for gene targeting. Nucleic Acids Res. 2008, 36:6333-6342.
9. Lee R.C., Gaylor D.C., Bhatt D., Israel D.A. Role of cell membrane rupture in the pathogenesis of electrical trauma. J. Surg. Res. 1988, 44:709-719.
10. Mir L.M. Nucleic acids electrotransfer-based gene therapy (electrogenetherapy): past, current, and future. Mol. Biotechnol. 2009, 43:167-176.
11. So S., Adachi N., Lieber M.R., Koyama H. Genetic interactions between BLM and DNA ligase IV in human cells. J. Biol. Chem. 2004, 279:55433-55442.
12. Toyoda E., et al. NK314, a topoisomerase II inhibitor that specifically targets the alpha isoform. J. Biol. Chem. 2008, 283:23711-23720.
13. Vasquez K.M., Marburger K., Intody Z., Wilson J.H. Manipulating the mammalian genome by homologous recombination. Proc. Natl. Acad. Sci. U. S. A. 2001, 98:8403-8410.