Human β-defensins differently affect proliferation, differentiation, and mineralization of osteoblast-like MG63 cells

Journal of Cellular Physiology

Volumn 227, Issue 3, 2012, Pages 994-1003

  Kraus, D ^a   Deschner, J ^a   Jager A ^a   Wenghoefer, M ^a   Bayer, S ^a   Jepsen, S ^a   Allam, J P ^a   Novak, N ^a   Meyer, R ^a   Winter, J ^a  

^a UNIVERSITY OF BONN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ALKALINE PHOSPHATASE; BETA DEFENSIN; BETA DEFENSIN 1; BETA DEFENSIN 2; BETA DEFENSIN 3; BIOLOGICAL MARKER; INTERLEUKIN 1BETA; INTERLEUKIN 6; MESSENGER RNA; NOTCH1 RECEPTOR; TOLL LIKE RECEPTOR 2; TOLL LIKE RECEPTOR 4; TUMOR NECROSIS FACTOR ALPHA;

AGONIST; ANTIMICROBIAL ACTIVITY; ARTICLE; BIOMINERALIZATION; BONE MINERALIZATION; CELL DIFFERENTIATION; CELL MATURATION; CELL PROLIFERATION; CELL STIMULATION; DOWN REGULATION; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; IMMUNOHISTOCHEMISTRY; INFLAMMATION; OSTEOBLAST; PRIORITY JOURNAL; PROTEIN CONTENT; PROTEIN EXPRESSION; PROTEIN FUNCTION; REAL TIME POLYMERASE CHAIN REACTION; TRANSCRIPTION INITIATION; UPREGULATION;

ANTIMICROBIAL CATIONIC PEPTIDES; BETA-DEFENSINS; BONE MORPHOGENETIC PROTEIN 2; BONE MORPHOGENETIC PROTEIN 4; CALCIFICATION, PHYSIOLOGIC; CELL DIFFERENTIATION; CELL LINE; CORE BINDING FACTOR ALPHA 1 SUBUNIT; HUMANS; OSTEOBLASTS; OSTEOGENESIS;

EID: 84155174651     PISSN: 00219541     EISSN: 10974652     Source Type: Journal    
DOI: 10.1002/jcp.22808     Document Type: Article

References (46)

1. Abiko Y, Nishimura M, Kusano K, Yamazaki M, Arakawa T, Takuma T, Kaku T. 2003. Upregulated expression of human β-defensin-1 and -3 mRNA during differentiation of keratinocyte immortalized cell lines, HaCaT and PHK16-0b. J Dermatol Sci 31: 225-228.
2. Aliprantis AO, Yang RB, Mark MR, Suggett S, Devaux B, Radolf JD, Klimpel GR, Godowski P, Zychlinsky A. 1999. Cell activation and apoptosis by bacterial lipoproteins through toll-like receptor-2. Science 285: 736-739.
3. Bandow K, Maeda A, Kakimoto K, Kusuyama J, Shamoto M, Ohnishi T, Matsuguchi T. 2010. Molecular mechanisms of the inhibitory effect of lipopolysaccharide (LPS) on osteoblast differentiation. Biochem Biophys Res Commun 402: 755-761.
4. Bensch KW, Raida M, Mägert HJ, Schulz-Knappe P, Forssmann WG. 1995. hBD-1: A novel β-defensin from human plasma. FEBS Lett 368: 331-335.
5. Bretaudiere JP, Spillman T. 1984. Alkaline phosphatases. In: Bergmeyer HU, editor. Methods of enzymatic analysis. Weinheim, Germany: Chemica. p 75-92.
6. Bullard RS, Gibson W, Bose SK, Belgrave JK, Eaddy AC, Wright CJ, Hazen-Martin DJ, Lage JM, Keane TE, Ganz TA, Donald CD. 2008. Functional analysis of the host defense peptide Human β-Defensin-1: New insight into its potential role in cancer. Mol Immunol 45: 839-848.
7. Duarte WR, Shibata T, Takenaga K, Takahashi E, Kubota K, Ohya K, Ishikawa I, Yamauchi M, Kasugai S. 2003. S100A4: A novel negative regulator of mineralization and osteoblast differentiation. J Bone Miner Res 18: 493-501.
8. Eda H, Shimada H, Beidler DR, Monahan JB. 2010. Proinflammatory cytokines, IL-1β and TNF-α, induce expression of interleukin-34 mRNA via JNK- and p44/42 MAPK-NF-κB pathway but not p38 pathway in osteoblasts. Rheumatol Int (epub ahead of print).
9. Darveau RP, Pham TT, Lemley K, Reife RA, Bainbridge BW, Coats SR, Howald WN, Way SS, Hajjar AM. 2004. Porphyromonas gingivalis lipopolysaccharide contains multiple lipid A species that functionally interact with both toll-like receptors 2 and 4. Infect Immun 72: 5041-5051.
10. Frye M, Bargon J, Gropp R. 2001. Expression of human β-defensin-1 promotes differentiation of keratinocytes. J Mol Med 79: 275-282.
11. Funderburg N, Lederman MM, Feng Z, Drage MG, Jadlowsky J, Harding CV, Weinberg A, Sieg SF. 2007. Human β-defensin-3 activates professional antigen-presenting cells via toll-like receptors 1 and 2. Proc Nat Acad Sci USA 104: 18631-18635.
12. Harder J, Bartels J, Christophers E, Schröder JM. 1997. A peptide antibiotic from human skin. Nature 387: 861.
13. Harder J, Bartels J, Christophers E, Schroder JM. 2001. Isolation and characterization of human β-defensin-3, a novel human inducible peptide antibiotic. J Biol Chem 276: 5707-5713.
14. Jung WK, Park IS, Park SJ, Yea SS, Choi YH, Oh S, Park SG, Choi IW. 2009. The 15-deoxy-Delta12, 14-prostaglandin J2 inhibits LPS-stimulated AKT and NF-κB activation and suppresses interleukin-6 in osteoblast-like cells MC3T3E-1. Life Sci 85: 46-53.
15. Kadono H, Kido J, Kataoka M, Yamauchi N, Nagata T. 1999. Inhibition of osteoblastic cell differentiation by lipopolysaccharide extract from Porphyromonas gingivalis Infect Immun 67: 2841-2846.
16. Kesting MR, Loeffelbein DJ, Hasler RJ, Wolff KD, Rittig A, Schulte M, Hirsch T, Wagenpfeil S, Jacobsen F, Steinstraesser L. 2009. Expression profile of human β-defensin 3 in oral squamous cell carcinoma. Cancer Invest 27: 575-581.
17. Kim H, Iwasaki K, Miyake T, Shiozawa T, Nozaki S, Yajima K. 2003. Changes in bone turnover markers during 14-day 6 degrees head-down bed rest. J Bone Miner Metab 21: 311-315.
18. Lacey DC, Simmons PJ, Graves SE, Hamilton JA. 2009. Proinflammatory cytokines inhibit osteogenic differentiation from stem cells: Implications for bone repair during inflammation. Osteoarthritis Cartilage 17: 735-742.
19. Lehrer RI. 2004. Primate defensins. Nat Rev Microbiol 2: 727-738.
20. Li J, Raghunath M, Tan D, Lareu RR, Chen Z, Beuerman RW. 2006. Defensins HNP1 and HBD2 stimulation of wound-associated responses in human conjunctival fibroblasts. Invest Ophthalm Vis Sci 47: 3811-3819.
21. Lossdörfer S, Schwartz Z, Lohmann CH, Greenspan DC, Ranly DM, Boyan BD. 2004. Osteoblast response to bioactive glasses in vitro correlates with inorganic phosphate content. Biomaterials 25: 2547-2555.
22. Lytvyn D, Zhuravel E, Markeeva N, Soldatkina M, Dudchenko T, Pogrebnoy P. 2006. Involvement of human β-defensin-2 in intracellular signaling: In vitro study. Exp Oncol 28: 198-202.
23. Mas JM, Aloy P, Martí-Renom MA, Oliva B, Blanco-Aparicio C, Molina MA, de Llorens R, Querol E, Avilés FX. 1998. Protein similarities beyond disulphide bridge topology. J Mol Biol 284: 541-548.
24. Mathews M, Jia HP, Guthmiller JM, Losh G, Graham S, Johnson GK, Tack BF, McCray PB. 1999. Production of β-defensin antimicrobial peptides by the oral mucosa and salivary glands. Infect Immun 67: 2740-2745.
25. Nishimura M, Abiko Y, Kurashige Y, Takeshima M, Yamazaki M, Kusano K, Saitoh M, Nakashima K, Inoue T, Kaku T. 2004. Effect of defensin peptides on eukaryotic cells: Primary epithelial cells, fibroblasts and squamous cell carcinoma cell lines. J Dermatol Sci 36: 87-95.
26. Niyonsaba F, Ushio H, Nakano N, Ng W, Sayama K, Hashimoto K, Nagaoka I, Okumura K, Ogawa H. 2007. Antimicrobial peptides human β-defensins stimulate epidermal keratinocyte migration, proliferation and production of proinflammatory cytokines and chemokines. J Invest Dermatol 127: 594-604.
27. Nomura I, Goleva E, Howell MD, Hamid QA, Ong PY, Hall CF, Darst MA, Gao B, Boguniewicz M, Travers JB, Leung DY. 2003. Cytokine milieu of atopic dermatitis, as compared to psoriasis, skin prevents induction of innate immune response genes. J Immunol 171: 3262-3269.
28. Pantelis A, Wenghoefer M, Haas S, Merkelbach-Bruse S, Pantelis D, Jepsen S, Bootz F, Winter J. 2009. Down regulation and nuclear localization of human β-defensin-1 in pleomorphic adenomas of salivary glands. Oral Oncol 45: 526-530.
29. Pfaffl MW. 2001. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acid Res 29: 2002-2007.
30. Presicce P, Giannelli S, Taddeo A, Villa ML, Della Bella S. 2009. Human defensins activate monocyte-derived dendritic cells, promote the production of proinflammatory cytokines, and up-regulate the surface expression of CD91. J Leukoc Biol 86: 941-948.
31. Sciaudone M, Gazzerro E, Priest L, Delany AM, Canalis E. 2003. Notch 1 impairs osteoblastic cell differentiation. Endocrinology 144: 5631-5639.
32. Strutz F, Okada H, Lo CW, Danoff T, Carone RL, Tomaszewski JE, Neilson EG. 1995. Identification and characterization of a fibroblast marker: FSP1. J Cell Biol 130: 393-405.
33. Sun CQ, Arnold R, Fernandez-Golarz C, Parrish AB, Almekinder T, He J, Ho SM, Svoboda P, Pohl J, Marshall FF, Petros JA. 2006. Human β-Defensin-1, a potential chromosome 8p tumor suppressor: Control of transcription and induction of apoptosis in renal cell carcinoma. Cancer Res 66: 8542-8549.
34. Varoga D, Pufe T, Harder J, Schröder JM, Mentlein R, Meyer-Hoffert U, Goldring MB, Tillmann B, Hassenpflug J, Paulsen F. 2005. Human β-defensin 3 mediates tissue remodeling processes in articular cartilage by increasing levels of metalloproteinases and reducing levels of their endogenous inhibitors. Arthritis Rheum 52: 1736-1745.
35. Varoga D, Tohidnezhad M, Paulsen F, Wruck CJ, Brandenburg L, Mentlein R, Lippross S, Hassenpflug J, Besch L, Müller M, Jürgens C, Seekamp A, Schmitt L, Pufe T. 2008. The role of human β-defensin-2 in bone. J Anat 213: 749-757.
36. Varoga D, Wruck CJ, Tohidnezhad M, Brandenburg L, Paulsen F, Mentlein R, Seekamp A, Besch L, Pufe T. 2009. Osteoblasts participate in the innate immunity of the bone by producing human β-defensin-3. Histochem Cell Biol 131: 207-218.
37. Wang YH, Jiang J, Zhu Q, AlAnezi AZ, Clark RB, Jiang X, Rowe DW, Nichols FC. 2010. Porphyromonas gingivalis lipids inhibit osteoblastic differentiation and function. Infect Immun 78: 3726-3735.
38. Warnke PH, Springer IN, Russo PA, Wiltfang J, Essig H, Kosmahl M, Sherry E, Acil Y. 2006. Innate immunity in human bone. Bone 38: 400-408.
39. Weinreb M, Shinar D, Rodan GA. 1990. Different pattern of alkaline phosphatase, osteopontin, and osteocalcin expression in developing rat bone visualized by in situ hybridization. J Bone Miner Res 5: 831-842.
40. Wenghoefer M, Pantelis A, Dommisch H, Götz W, Reich R, Bergé S, Martini M, Allam JP, Jepsen S, Merkelbach-Bruse S, Fischer HP, Novak N, Winter J. 2008a. Nuclear hBD-1 accumulation in malignant salivary gland tumours. BMC Cancer 8: 290.
41. Wenghoefer M, Pantelis A, Dommisch H, Reich R, Martini M, Allam JP, Novak N, Bergé S, Jepsen S, Winter J. 2008b. Decreased gene expression of human β-defensin-1 in the development of squamous cell carcinoma of the oral cavity. Int J Oral Maxillofac Surg 37: 660-663.
42. Winter J, Pantelis A, Reich R, Martini M, Kraus D, Jepsen S, Allam JP, Novak N, Wenghoefer M. 2011. Human β-defensin-1, -2 and -3 exhibit opposite effects on oral squamous cell carcinoma cell proliferation. Cancer Invest 29: 196-201.
43. Xing Q, Ye Q, Fan M, Zhou Y, Xu Q, Sandham A. 2010. Porphyromonas gingivalis lipopolysaccharide inhibits the osteoblastic differentiation of preosteoblasts by activating Notch1 signaling. J Cell Physiol 225: 106-114.
44. Yang D, Chertov O, Bykovskaia SN, Chen Q, Buffo MJ, Shogan J, Anderson J M, Schröder JM, Wang JM, Howard OM, Oppenheim JJ. 1999. β-defensins: Linking innate and adaptive immunity through dendritic and T cell CCR6. Science 286: 525-528.
45. Yang D, Liu ZH, Tewary P, Chen Q, de la Rosa G, Oppenheim JJ. 2007. Defensin participation in innate and adaptive immunity. Curr Pharm Des 13: 3131-3139.
46. Zanotti S, Smerdel-Ramoya A, Stadmeyer L, Durant D, Radtke F, Canalis E. 2008. Notch inhibits osteoblast differentiation and causes osteopenia. Endocrinology 149: 3890-3899.