Anthrax sub-unit vaccine: The structural consequences of binding rPA83 to Alhydrogel®

European Journal of Pharmaceutics and Biopharmaceutics

Volumn 80, Issue 1, 2012, Pages 25-32

  Soliakov, Andrei ^a   Kelly, Ian F ^a   Lakey, Jeremy H ^a   Watkinson, Allan ^b,c  

^a NEWCASTLE UNIVERSITY   (United Kingdom)

^b PharmAthene UK Ltd   (United Kingdom)

^c GSB Pharma Consultants Ltd   (United Kingdom)

Author keywords

Alhydrogel; Anthrax; Calorimetry; Fluorescence; PA83; Sub unit vaccine

Indexed keywords

ALUMINUM HYDROXIDE; ANTHRAX VACCINE; CALCIUM ION; RECOMBINANT ANTIGEN; RECOMBINANT PROTECTIVE ANTIGEN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DIFFERENTIAL SCANNING CALORIMETRY; DRUG ABSORPTION; DRUG BINDING; DRUG STABILITY; DRUG STRUCTURE; ENTHALPY; FLUORESCENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DEGRADATION; PROTEIN FOLDING; PROTEIN STRUCTURE; PROTEIN TERTIARY STRUCTURE; PROTEIN UNFOLDING; TEMPERATURE;

ADJUVANTS, IMMUNOLOGIC; ADSORPTION; ALUMINUM HYDROXIDE; ANTHRAX VACCINES; CALCIUM; CALORIMETRY, DIFFERENTIAL SCANNING; LINEAR MODELS; MODELS, MOLECULAR; PROTECTIVE AGENTS; PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; PROTEIN UNFOLDING; PROTEOLYSIS; RECOMBINANT PROTEINS; TEMPERATURE; THERMODYNAMICS; UREA;

EID: 83955164227     PISSN: 09396411     EISSN: 18733441     Source Type: Journal    
DOI: 10.1016/j.ejpb.2011.09.009     Document Type: Article

References (44)

1. P.C.B. Turnbull Anthrax vaccines - past, present and future Vaccine 9 1991 533 539
2. S.A. Plotkin Vaccines: past, present and future Nat. Med. 11 2005 S5 S11
3. S. Liljeqvist, and S. Stahl Production of recombinant subunit vaccines: protein immunogens live, delivery systems and nucleic acid vaccines J. Biotechnol. 73 1999 1 33 (Pubitemid 29347186)
4. D.T. O'Hagan, M.L. MacKichan, and M. Singh Recent developments in adjuvants for vaccines against infectious diseases Biomol. Eng. 18 2001 69 85 (Pubitemid 32889607)
5. E.D. Williamson, I. Hodgson, N.J. Walker, A.W. Topping, M.G. Duchars, J.M. Mott, J. Estep, C. LeButt, H.C. Flick-Smith, H.E. Jones, H. Li, and C.P. Quinn Immunogenicity of recombinant protective antigen and efficacy against aerosol challenge with anthrax Infect. Immun. 73 2005 5978 5987 (Pubitemid 41193036)
6. R. Edelman Vaccine adjuvants Rev. Infect. Dis. 2 1980 370 383
7. E.B. Lindblad Aluminium compounds for use in vaccines Immunol. Cell Biol. 82 2004 497 505 (Pubitemid 39421059)
8. A.T. Glenny, C.G. Pope, H. Waddington, and U. Wallace The antigenic value of toxoid precipitated by potassium alum J. Pathol. Bacteriol. 29 1926 38 45
9. R.L. Hunter Overview of vaccine adjuvants: present and future Vaccine 20 2002 S7 S12
10. J.W. Mannhalter, H.O. Neychev, G.J. Zlabinger, R. Ahmad, and M.M. Eibl Modulation of the human immune-response by the non-toxic and non-pyrogenic adjuvant aluminum hydroxide - effect on antigen uptake and antigen presentation Clin. Exp. Immunol. 61 1985 143 151 (Pubitemid 15029859)
11. G.L. Morefield, A. Sokolovska, D.P. Jiang, H. Hogenesch, J.P. Robinson, and S.L. Hem Role of aluminum-containing adjuvants in antigen internalization by dendritic cells in vitro Vaccine 23 2005 1588 1595 (Pubitemid 40203396)
12. T.L. Flach, G. Ng, A. Hari, M.D. Desrosiers, P. Zhang, S.M. Ward, M.E. Seamone, A. Vilaysane, A.D. Mucsi, Y. Fong, E. Prenner, C.C. Ling, J. Tschopp, D.A. Muruve, M.W. Amrein, and Y. Shi Alum interaction with dendritic cell membrane lipids is essential for its adjuvanticity Nat. Med. 17 2011 479 487
13. M. Kool, T. Soullie, M. van Nimwegen, M.A.M. Willart, F. Muskens, S. Jung, H.C. Hoogsteden, H. Hammad, and B.N. Lambrecht Alum adjuvant boosts adaptive immunity by inducing uric acid and activating inflammatory dendritic cells J. Exp. Med. 205 2008 869 882 (Pubitemid 351549884)
14. L.S. Jones, L.J. Peek, J. Power, A. Markham, B. Yazzie, and C.R. Middaugh Effects of adsorption to aluminum salt adjuvants on the structure and stability of model protein antigens J. Biol. Chem. 280 2005 13406 13414 (Pubitemid 40517228)
15. S.F. Bai, and A.C. Dong Effects of immobilization onto aluminum hydroxide particles on the thermally induced conformational behavior of three model proteins Int. J. Biol. Macromol. 45 2009 80 85
16. A. Dong, L.S. Jones, B.A. Kerwin, S. Krishnan, and J.F. Carpenter Secondary structures of proteins adsorbed onto aluminum hydroxide: infrared spectroscopic analysis of proteins from low solution concentrations Anal. Biochem. 351 2006 282 289
17. M.A.H. Capelle, P. Brügger, and T. Arvinte Spectroscopic characterization of antibodies adsorbed to aluminium adjuvants: correlation with antibody vaccine immunogenicity Vaccine 23 2005 1686 1694 (Pubitemid 40215702)
18. A. Soliakov, J.R. Harris, A. Watkinson, and J.H. Lakey The structure of Yersinia pestis Caf1 polymer in free and adjuvant bound states Vaccine 28 2010 5746 5754
19. P.C.B. Turnbull, M.G. Broster, J.A. Carman, R.J. Manchee, and J. Melling Development of antibodies to protective antigen and lethal factor components of anthrax toxin in humans and guinea-pigs and their relevance to protective immunity Infect. Immun. 52 1986 356 363 (Pubitemid 16117786)
20. C. Petosa, J.R. Collier, K.R. Klimpel, S.H. Leppla, and R.C. Liddington Crystal structure of the anthrax toxin protective antigen Nature 385 1997 833 838 (Pubitemid 27106075)
21. D.A. Chalton, I.F. Kelly, A. McGregor, H. Ridley, A. Watkinson, J. Miller, and J.H. Lakey Unfolding transitions of Bacillus anthracis protective antigen Arch. Biochem. Biophys. 465 2007 1 10 (Pubitemid 47299083)
22. R.A.L. Pimental, K.A. Christensen, B.A. Krantz, and R.J. Collier Anthrax toxin complexes: heptameric protective antigen can bind lethal factor and edema factor simultaneously Biochem. Biophys. Res. Commun. 322 2004 258 262 (Pubitemid 39093863)
23. C.J. Miller, J.L. Elliott, and R.J. Collier Anthrax protective antigen: prepare-to-pore conversion Biochemistry 38 1999 10432 10441
24. J. Mogridge, M. Mourez, and R.J. Collier Involvement of domain 3 in oligomerization by the protective antigen moiety of anthrax toxin J. Bacteriol. 183 2001 2111 2116 (Pubitemid 32229126)
25. M. Varughese, A.V. Teixeira, S.H. Liu, and S.H. Leppla Identification of a receptor-binding region within domain 4 of the protective antigen component of anthrax toxin Infect. Immun. 67 1999 1860 1865 (Pubitemid 29144407)
26. M.J. Rosovitz, P. Schuck, M. Varughese, A.P. Chopra, V. Mehra, Y. Singh, L.M. McGinnis, and S.H. Leppla Alanine-scanning mutations in domain 4 of anthrax toxin protective antigen reveal residues important for binding to the cellular receptor and to a neutralizing monoclonal antibody J. Biol. Chem. 278 2003 30936 30944 (Pubitemid 36994605)
27. S.L. Wang, C.T. Johnson, D.L. Bish, J.L. White, and S.L. Hem Water-vapor adsorption and surface area measurement of poorly crystalline boehmite J. Colloid Interface Sci. 260 2003 26 35 (Pubitemid 36398184)
28. C.T. Johnston, S.L. Wang, and S.L. Hem Measuring the surface area of aluminum hydroxide adjuvant J. Pharm. Sci. 91 2002 1702 1706 (Pubitemid 34680233)
29. P.K. Yau, D.G. Schulze, C.T. Johnston, and S.L. Hem Aluminum hydroxide adjuvant produced under constant reactant concentration J. Pharm. Sci. 95 2006 1822 1833 (Pubitemid 44229588)
30. S. Seeber, J.L. White, and S.L. Hem Predicting the adsorption of proteins by aluminium-containing adjuvants Vaccine 9 1991 201 203
31. E.H. Dyson, M.S. Irving, R. Goldwater, R. Stolz, M. Duchars, A.E. Lockert, S. A.J.H., Ascending dose study to assess the safety and tolerability of an rPA anthrax vaccine, and compare its immunogenicity with that of anthrax vaccine adsorbed (AVA), ICAAC 2006 San Francisco, 2006.
32. I. Langmuir The constitution and fundamental properties of solids and liquids: Part 1. Solids. J. Am. Chem. Soc. 38 1916 2221 2295
33. J.H. Lakey, and M. Ptak Fluorescence indicates a calcium-dependent interaction between the lipopeptide antibiotic LY146032 and phospholipid membranes Biochemistry 27 1988 4639 4645
34. S.S. Lehrer Solute perturbation of protein fluorescence - quenching of tryptophyl fluorescence of model compounds and of lysozyme by iodide ion Biochemistry 10 1971 3254
35. C.N. Pace, B.A. Shirley, and J.A. Thompson Measuring the conformational stability of a protein T.E. Creighton, Protein Structure; A Practical Approach 1989 IRL Press Oxford 311 330
36. C. Bustamante, and M.F. Maestre Statistical effects in the absorption and optical activity of particulate suspensions PNAS 85 1988 8482 8486
37. P.K. Gupta, H. Chandra, R. Gaur, R.K. Kurupati, S. Chowdhury, V. Tandon, Y. Singh, and K. Maithal Conformational fluctuations in anthrax protective antigen: a possible role of calcium in the folding pathway of the protein FEBS Lett. 554 2003 505 510 (Pubitemid 37412540)
38. J.M. Novak, M.P. Stein, S.F. Little, S.H. Leppla, and A.M. Friedlander Functional-characterization of protease treated Bacillus-anthracis protective antigen J. Biol. Chem. 267 1992 17186 17193
39. D.L. Sackett, and J. Wolff Nile red as a polarity sensitive fluorescent probe of hydrophobic protein surfaces Anal. Biochem. 167 1987 228 234
40. S. Gao-Sheridan, S. Zhang, and R.J. Collier Exchange characteristics of calcium ions bound to anthrax protective antigen Biochem. Biophys. Res. Commun. 300 2003 61 64 (Pubitemid 36042930)
41. A. Fontana, P.P. de Laureto, B. Spolaore, E. Frare, P. Picotti, and M. Zambonin Probing protein structure by limited proteolysis Acta Biochim. Pol. 51 2004 299 321 (Pubitemid 38969296)
42. A. Cooper Thermodynamics of protein folding and stability G. Allan, Protein: Aa Comprehensive Treatise (Physical and Chemical Properties of Proteins) vol. 2 1999 JAI Press Inc. Stamford
43. H.S. Renate Forch, A. Tobias A. Jenkins (Ed.), Surface Design: Application in Bioscience and Nanotechnology, Wiley VCH Verlag GmbH & Co. KGaA, Weinheim, 2009.
44. R. Thai, G. Moine, M. Desmadril, D. Servent, J.L. Tarride, A. Menez, and M. Leonetti Antigen stability controls antigen presentation J. Biol. Chem. 279 2004 50257 50266 (Pubitemid 39577840)