A polyphasic approach for the characterization of endophytic Alternaria strains isolated from grapevines

Journal of Microbiological Methods

Volumn 88, Issue 1, 2012, Pages 162-171

  Polizzotto, Rachele ^a   Andersen, Birgitte ^b,c   Martini, Marta ^a   Grisan, Simone ^a   Assante, Gemma ^d   Musetti, Rita ^a  

^a UNIVERSITY OF UDINE   (Italy)

^b TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^c Università di Milano ^*  (Denmark)

^d UNIVERSITY OF MILAN   (Italy)

Author keywords

Chemotaxonomy; Metabolite profiling; Morphology; RAPD

Indexed keywords

FUNGAL DNA;

ALTERNARIA; ALTERNARIA ALTERNATA; ALTERNARIA ARBORESCENS; ALTERNARIA INCOMPLEXA; ALTERNARIA INFECTORIA; ALTERNARIA TENUISSIMA; ARTICLE; CHEMOTAXONOMY; CONTROLLED STUDY; ENDOPHYTE; FUNGAL METABOLISM; FUNGAL STRAIN; FUNGAL STRUCTURES; FUNGUS IDENTIFICATION; GRAPE; ITALY; MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; SPECIES COMPARISON; SPOROGENESIS;

ALTERNARIA; ALTERNARIA ALTERNATA; ALTERNARIA ARBORESCENS; ALTERNARIA TENUISSIMA; VITIS;

EID: 83955161064     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2011.11.009     Document Type: Article

References (47)

1. Aly A.H., Edrada-Ebel R.A., Indriani I.D., Wray V., Muller W.E.G., Totzke F., Zirrgiebel U., Schachtele C., Kubbutat M.H.G., Lin W.H., Proksch P., Ebel R. Cytotoxic metabolites from the fungal endophyte Alternaria sp. and their subsequent detection in its host plant Polygonum senegalense. J. Nat. Prod. 2008, 71:972-980.
2. Andersen B., Thrane U. Differentiation of Alternaria infectoria and Alternaria alternata based on morphology, metabolites profiles and cultural characteristics. Can. J. Microbiol. 1996, 42:685-689.
3. Andersen B., Krøger E., Roberts R.G. Chemical and morphological segregation of Alternaria alternata, A. gaisen and A. longipes. Mycol. Res. 2001, 105:291-299.
4. Andersen B., Krøger E., Roberts R.G. Chemical and morphological segregation of Alternaria arborescens, A. infectoria and A. tenuissima species-groups. Mycol. Res. 2002, 106:170-182.
5. Andersen B., Hansen M.E., Smedsgaard J. Automated and unbiased image analyses as tools in phenotypic classification of small-spored Alternaria spp. Phytopathology 2005, 95:1021-1029.
6. Andersen B., Sørensen J.L., Nielsen K.F., Gerrits van den Ende B., de Hoog S. A polyphasic approach to the taxonomy of the Alternaria infectoria species-group. Fungal Genet. Biol. 2009, 46:642-656.
7. Andrew M., Peever T.L., Pryor B.M. An expanded multilocus phylogeny does not resolve morphological species within the small-spored Alternaria species complex. Mycologia 2009, 101:95-109.
8. Azevedo J.L., Maccheroni W., Pereira J.O., de Araujo W.L. Endophytic microorganisms: a review on insect control and recent advances on tropical plants. Electron. J. Biotechnol. 2000, 3:1-36.
9. Bottalico A., Logrieco A. Alternaria plant disease in Mediterranean countries and associated mycotoxins. Alternaria Biology, Plant Disease and Metabolites 1992, 209-232. Elsevier, Amsterdam, The Netherlands. J. Chelkowski, A. Visconti (Eds.).
10. Bruns T.D., White T.J., Taylor J.W. Fungal molecular systematic. Annu. Rev. Ecol. Syst. 1991, 22:525-564.
11. Bulgari D., Casati P., Crepaldi P., Daffonchio D., Quaglino F., Brusetti L., Bianco P.A. Restructuring of endophytic bacterial communities in grapevine yellows-diseased and recovered Vitis vinifera L. plants. Appl. Environ. Microbiol. 2011, 77:5018-5022.
12. Cooke D.E.L., Forster J.W., Jenkins P.D., Jones D.G., Lewis D.M. Analysis of intraspecific and interspecific variation in the genus Alternaria by the use of RAPD-PCR. Ann. Appl. Biol. 1998, 132:197-209.
13. Cota B.B., Rosa L.H., Caligiorne R.B., Rabello A.L., Teles A., Alves T.M., Rosa C.A., Zani C.L. Altenusin, a biphenyl isolated from the endophytic fungus Alternaria sp., inhibits trypanothione reductase from Trypanosoma cruzi. FEMS Microbiol. Lett. 2008, 285:177-182.
14. Dugan F.M., Lupien S.L. Filamentous fungi quiescent in seeds and culm nodes of weedy and forage grass species endemic to the Palouse Region of Washington and Idaho. Mycopahologia 2002, 156:31-40.
15. Dugan F.M., Lupien S.L., Grove G.G. Incidence, aggressiveness and in planta interactions of Botrytis cinerea and other filamentous fungi quiescent in grape berries and dormant buds in Central Washington State. J. Pythopathol. 2002, 150:375-381.
16. Frisvad J.C. A selective and indicative medium for groups of Penicillium viridicatum producing different mycotoxins on cereals. J. Appl. Bacteriol. 1983, 54:409-416.
17. Frisvad J.C., Thrane U. Standardized high-performance liquid chromatography of 182 mycotoxins and other fungal metabolites based on alkylphenone retention indices and UV-VIS spectra (diode array detection). J. Chromatogr. 1987, 404:195-214.
18. Frisvad J.C., Andersen B., Thrane U. The use of secondary metabolite profiling in chemotaxonomy of filamentous fungi. Mycol. Res. 2008, 112:231-240.
19. Grisan S., Martini M., Musetti R., Osler R. Development of a molecular approach to describe the diversity of fungal endophytes in either phytoplasma-infected, recovered or healthy grapevines. Bull. Insectol. 2011, 64:207-208.
20. Guo L.D., Xu L., Zheng W.H., Hyde K.D. Genetic variation of Alternaria alternata, an endophytic fungus isolated from Pinus tabulaeformis as determined by random amplified microsatellites (RAMS). Fund. Div. 2004, 16:53-65.
21. Hansen, M.E. 2003. Indexing and analysis of fungal phenotypes using morphology and spectrometry. Ph.D. thesis. ISSN 0909-3192, IMM, DTU, Denmark.
22. Hellwig V., Grothe T., Mayer-Bartscmid A., Endermann R., Geschke F.U., Henkel T., Stadler M. Altersetin, a new antibiotic from cultures of endophytic Alternaria spp. Taxonomy, fermentation, isolation, structure elucidation and biological activities. J. Antibiot. 2002, 55:881-892.
23. Hong S.G., Liu D., Pryor B.M. Restriction mapping of the IGS region in Alternaria spp. reveals variable and conserved domains. Mycol. Res. 2005, 109:87-95.
24. Hong S.G., Maccaroni M., Figuli P.J., Pryor B.M., Belisario A. Polyphasic classification of Alternaria isolated from hazelnut and walnut fruit in Europe. Mycol. Res. 2006, 110:1290-1300.
25. Kornerup A., Wanscher J.H. Methuen Handbook Of Color 1978, E. Methuen, London, U. K.
26. Kortekamp A. Epicoccum nigrum Link: a biological control agent of Plasmopara viticola (Berk. et Curt.) Berl. et De Toni. Vitis 1997, 36:215-216.
27. Kusaba M., Tsuge T. Phologeny of Alternaria fungi known to produce host-specific toxins on the basis of variation in internal transcribed spacers of ribosomal DNA. Curr. Genet 1995, 28:491-498.
28. Lecellier G., Silar P. Rapid methods for nucleic acids extraction from Petri dish-grown mycelia. Curr. Genet 1994, 25:122-123.
29. Martini M., Musetti R., Grisan S., Polizzotto R., Borselli S., Pavan F., Osler R. DNA-dependent detection of the grapevine fungal endophytes. Plant Dis. 2009, 93:993-998.
30. Mostert L., Crous P.W., Petrini O. Endophytic fungi associated with shoots and leaves of Vitis vinifera, with specific reference to the Phomopsis viticola complex. Sydowia 2000, 52:46-58.
31. Musetti R., Vecchione A., Stringher L., Borselli S., Zulini L., Marzani C., D'Ambrosio M., Sanità di Toppi L., Pertot I. Inhibition of sporulation and ultrastructural alterations of grapevine downy mildew by the endophytic fungus Alternaria alternata. Phytopathology 2006, 96:689-698.
32. Musetti R., Polizzotto R., Vecchione A., Borselli S., Zulini L., D'Ambrosio M., Sanità di Toppi L., Pertot I. Antifungal activity of diketopiperazines extracted from Alternaria alternata against Plasmopara viticola: An ultrastructural study. Micron 2007, 38:643-650.
33. Nielsen K.F., Smedsgaard J. Fungal metabolite screening: database of 474 mycotoxins and fungal metabolites for dereplication by standardised liquid chromatography-UV-mass spectrometry methodology. J. Chromatogr. 2003, 1002:111-136.
34. Peever T.L., Su G., Carpenter-Boggs L., Timmer L.W. Molecular systematics of citrus-associated Alternaria species. Mycologia 2004, 96:119-134.
35. Polizzotto R., D'Agostin S., Grisan S., Assante G., Pertot I., Andersen B., Musetti R. Attività di ceppi endofitici di Alternaria spp. nel controllo di Plasmopara viticola. J. Plant Pathol. 2009, 91(4 Supplementum):79-80.
36. Pryor B.M., Bigelow D.M. Molecular characterization of Embellisia and Nimbya species and their relationship to Alternaria, Ulocladium and Stemphylium. Mycologia 2003, 95:1141-1154.
37. Pryor B.M., Michailides T.J. Morphological, pathogenic, and molecular characterization of Alternaria isolates associated with Alternaria late blight of pistachio. Phytopathology 2002, 92:406-416.
38. Quiao L.-R., Lin Y., Gao -M., Zhao P.-J., Kang Q.-J., Shen Y.-M. Tricycloalternarene derivatives produced by an endophyte Alternaria alternata isolated from Maytenus hookeri. J. Basic Microbiol. 2007, 47:340-343.
39. Roberts R.G., Reymond S.T., Andersen B. RAPD fragment pattern analysis and morphological segregation of small-spored Alternaria species and species groups. Mycol. Res. 2000, 104:151-160.
40. Samson R.A., Hoekstra E.S., Frisvad J.C., Filtenborg O. Introduction to food- and air borne fungi 2002, Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands, pp 378-382.
41. Schena L., Ippolito A., Zahavi T., Cohen L., Nigro F., Droby S. Genetic diversity and biocontrol activity of Aureobasidium pullulans isolates against postharvest rots. Postharvest Biol. Technol. 1999, 17:189-199.
42. Schulz B., Boyle C., Draegher S., Römmert A.K., Krohn K. Endophytic fungi: a source of novel biologically active secondary metabolites. Mycol. Res. 2002, 9:996-1004.
43. Simmons E.G. Alternaria taxonomy: current status, viewpoint, challenge. Alternaria Biology, Plant Disease and Metabolites 1992, 1-35. Elsevier, Amsterdam, The Netherlands. J. Chelkowski, A. Visconti (Eds.).
44. Simmons E.G., Roberts R.G. Alternaria themes and variations. Mycotaxon 1993, 48:109-140.
45. Su Y.-Y., Guo L.-D., Hyde K.D. Response of endophytic fungi of Stipa grandis to experimental plant function group removal in Inner Mongolia steppe. China. Fund. Div. 2010, 43:93-101.
46. Tan R.X., Zou W.X. Endophytes: a rich source of functional metabolites. Nat. Prod. 2001, 18:448-459.
47. Wold S., Sjöström M., Eriksson L. PLS-regression: a basic tool of chemometrics. Chemom. Intell. Lab. Sys. 2001, 58:109-130.