Rapid and sensitive detection of Shiga toxin-producing escherichia coli directly from stool samples by real-time PCR in comparison to culture, enzyme immunoassay and vero cell cytotoxicity assay

Clinical Laboratory

Volumn 57, Issue 11-12, 2011, Pages 993-998

  Gerritzen, Andreas ^a   Wittke, Johann Wolfgang ^a   Wolff, Dietmar ^a  

^a Medizinisches Labor Bremen   (Germany)

Author keywords

Broth enrichment; Culture; Cytotoxicity assay; Enzyme immunoassay; Rapid diagnosis; Real time PCR; Shiga toxin

Indexed keywords

EXTENDED SPECTRUM BETA LACTAMASE; HEMOLYSIN; VEROTOXIN 1; VEROTOXIN 2;

ARTICLE; BACTERIUM ISOLATE; CELL CULTURE; COMPARATIVE STUDY; CYTOTOXICITY TEST; DIAGNOSTIC TEST ACCURACY STUDY; DIARRHEA; ENZYME IMMUNOASSAY; ESCHERICHIA COLI INFECTION; FECES; LABORATORY DIAGNOSIS; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; VERO CELL;

ANIMALS; BACTERIOLOGICAL TECHNIQUES; CERCOPITHECUS AETHIOPS; CULTURE MEDIA; DIARRHEA; DISEASE OUTBREAKS; DNA, BACTERIAL; EARLY DIAGNOSIS; ESCHERICHIA COLI INFECTIONS; FECES; GERMANY; HEMOLYTIC-UREMIC SYNDROME; HUMANS; IMMUNOENZYME TECHNIQUES; INPATIENTS; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SHIGA TOXIN 1; SHIGA TOXIN 2; SHIGA-TOXIGENIC ESCHERICHIA COLI; VERO CELLS;

EID: 83455212158     PISSN: 14336510     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

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