Spheroid assay to measure TGF-β-induced invasion

Journal of Visualized Experiments

Volumn , Issue 57, 2011, Pages

  Naber, Hildegonda P H ^a   Wiercinska, Eliza ^a   Dijke, Peter Ten ^a   van Laar, Theo ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

Assay; Breast cancer; Collagen; Invasion; Issue 57; Medicine; Oncology; Spheroids; TGF; TGF

Indexed keywords

EID: 83455195415     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/3337     Document Type: Article

References (23)

1. Akhurst, R.J. & Derynck, R. TGF-β signaling in cancer--a double-edged sword. Trends. Cell. Biol. 11 (11), S44-S51 (2001).
2. Massagué, J. TGF-β in Cancer. Cell. 134 (2), 215 (2008).
3. Ghellal, A., et al. Prognostic significance of TGF-β1 and TGF-β in human breast carcinoma. Anticancer. Res. 20 (6B), 4413 (2000).
4. Sheen-Chen, S.M., et al. Serum levels of transforming growth factor-β1 in patients with breast cancer. Arch. Surg. 136 (8), 937 (2001).
5. ten Dijke, P. & Hill, C.S. New insights into TGF-β-Smad signaling. Trends Biochem. Sci. 29 (5), 265 (2004).
6. Moustakas, A. & Heldin, C.H. Non-Smad TGF-β signals. J. Cell Sci. 118 (Pt 16), 3573 (2005).
7. Soule, H.D., et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50 (18), 6075 (1990).
8. Strickland, L.B., et al. Progression of premalignant MCF10AT generates heterogeneous malignant variants with characteristic histologic types and immunohistochemical markers. Breast Cancer Res. Treat. 64 (3), 235 (2000).
9. Santner, S.J., et al. Malignant MCF10CA1 cell lines derived from premalignant human breast epithelial MCF10AT cells. Breast Cancer Res. Treat. 65 (2), 101 (2001).
10. Smalley, K.S., Lioni, M., & Herlyn, M. Life isn't flat: taking cancer biology to the next dimension. In Vitro Cell Dev. Biol. Anim. 42 (8-9), 242 (2006).
11. Kobayashi, H., et al. Acquired multicellular-mediated resistance to alkylating agents in cancer. Proc. Natl. Acad. Sci. U. S. A. 90 (8), 3294 (1993).
12. Inman, G.J., et al. SB-431542 is a potent and specific inhibitor of transforming growth factor-b superfamily type I activin receptor-like kinase (ALK) receptors ALK4, ALK5, and ALK7. Mol. Pharmacol. 62, 65 (2002).
13. Wiercinska, E., et al. The TGF-β/Smad pathway induces breast cancer cell invasion through the up-regulation of matrix metalloproteinase 2 and 9 in a spheroid invasion model system. Breast Cancer Res. Treat. 128, 657 (2011).
14. Tang, B., et al. TGF-β switches from tumor suppressor to prometastatic factor in a model of breast cancer progression. J. Clin. Invest. 112(7), 1116 (2003).
15. Provenzano, P.P., et al. Collagen density promotes mammary tumor initiation and progression. BMC. Med. 6, 11 (2008).
16. Ramaswamy, S., et al. A molecular signature of metastasis in primary solid tumors. Nat. Genet. 33 (1), 49 (2003).
17. Debnath, J. & Brugge, J.S. Modelling glandular epithelial cancers in three-dimensional cultures. Nat. Rev. Cancer 5 (9), 675 (2005).
18. Pampaloni, F., Reynaud, E.G., & Stelzer, E.H. The third dimension bridges the gap between cell culture and live tissue. Nat. Rev. Mol. Cell. Biol. 8 (10), 839 (2007).
19. Lee, G.Y., et al. Three-dimensional culture models of normal and malignant breast epithelial cells. Nat. Methods. 4 (4), 359 (2007).
20. Petersen, O.W., et al. Interaction with basement membrane serves to rapidly distinguish growth and differentiation pattern of normal and malignant human breast epithelial cells. Proc. Natl. Acad. Sci. U. S. A. 89 (19), 9064 (1992).
21. Debnath, J., Muthuswamy, S.K., & Brugge J.S. Morphogenesis and oncogenesis of MCF10A mammary epithelial acini grown in three-dimensional basement membrane cultures. 30, 256 (2011).
22. Li, Q., et al. p21-Activated kinase 1 coordinates aberrant cell survival and pericellular proteolysis in a three-dimensional culture model for premalignant progression of human breast cancer. 10, 314 (2011).
23. Li, Q., Chow, A.B., & Mattingly, R.R. Three-dimensional overlay culture models of human breast cancer reveal a critical sensitivity to mitogen-activated protein kinase kinase inhibitors. J. Pharmacol. Exp. Ther. 332 (3), 821 (2010).