Improvement in the thermostability of D-psicose 3-epimerase from Agrobacterium tumefaciens by random and site-directed mutagenesis

Applied and Environmental Microbiology

Volumn 77, Issue 20, 2011, Pages 7316-7320

  Choi, Jin Geun ^a   Ju, Yo Han ^a   Yeom, Soo Jin ^a   Oh, Deok Kun ^a  

^a Konkuk University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

AGROBACTERIUM TUMEFACIENS; AROMATIC STACKING INTERACTIONS; CONVERSION YIELD; OPERATION TIME; PSICOSE; SITE DIRECTED MUTAGENESIS; WILD-TYPE ENZYMES;

HYDROGEN BONDS; MUTAGENESIS;

ENZYME ACTIVITY;

FRUCTOSE; IMMOBILIZED ENZYME; ISOMERASE; MUTANT PROTEIN; PSICOSE;

BACTERIUM; CHEMICAL BONDING; ENZYME ACTIVITY; HYDROGEN; MELTING; MUTAGENICITY; STABILIZATION; TEMPERATURE EFFECT;

AMINO ACID SUBSTITUTION; ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; METABOLISM; MUTAGENESIS; PROTEIN STABILITY; RHIZOBIUM RADIOBACTER; TEMPERATURE; TRANSITION TEMPERATURE;

AGROBACTERIUM TUMEFACIENS; AMINO ACID SUBSTITUTION; ENZYME STABILITY; ENZYMES, IMMOBILIZED; FRUCTOSE; MODELS, MOLECULAR; MUTAGENESIS; MUTANT PROTEINS; PROTEIN STABILITY; RACEMASES AND EPIMERASES; TEMPERATURE; TRANSITION TEMPERATURE;

EID: 82955240007     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.05566-11     Document Type: Article

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