Enhancement of the thermostability of a recombinant β-agarase, AgaB, from Zobellia galactanivorans by random mutagenesis

Biotechnology Letters

Volumn 32, Issue 7, 2010, Pages 943-949

  Jang, Min Kyung ^a   Lee, Seung Woo ^a   Lee, Dong Geun ^a   Kim, Nam Young ^a   Yu, Ki Hwan ^a   Jang, Hye Ji ^a   Kim, Suhkman ^b   Kim, Andre ^a   Lee, Sang Hyeon ^a  

^a Silla University   (South Korea)

^b Pusan National University   (South Korea)

Author keywords

Agarase AgaB; Random mutagenesis; Thermostability; Zobellia galactanivorans

Indexed keywords

CATALYTIC ACTIVITY; CD SPECTRA; MELTING TEMPERATURES; MUTANT ENZYMES; RANDOM MUTAGENESIS; WILD TYPES; WILD-TYPE ENZYMES;

CALCIUM CHLORIDE; CATALYSTS; MUTAGENESIS;

ENZYMES;

AGARASE; BACTERIAL PROTEIN; CALCIUM CHLORIDE; GLYCOSIDASE; MUTANT PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; CHEMISTRY; CIRCULAR DICHROISM; ENZYME STABILITY; ENZYMOLOGY; FLAVOBACTERIACEAE; GENETICS; HEAT; METABOLISM; MISSENSE MUTATION; MUTAGENESIS; NUCLEOTIDE SEQUENCE; PROTEIN STABILITY; TRANSITION TEMPERATURE;

AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; CALCIUM CHLORIDE; CIRCULAR DICHROISM; DNA MUTATIONAL ANALYSIS; ENZYME STABILITY; FLAVOBACTERIACEAE; GLYCOSIDE HYDROLASES; HOT TEMPERATURE; MUTAGENESIS; MUTANT PROTEINS; MUTATION, MISSENSE; PROTEIN STABILITY; TRANSITION TEMPERATURE;

ZOBELLIA GALACTANIVORANS;

EID: 77953869815     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-010-0237-5     Document Type: Article

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