DNA electrophoretic migration patterns change after exposure of jurkat cells to a single intense nanosecond electric pulse

PLoS ONE

Volumn 6, Issue 12, 2011, Pages

  Romeo, Stefania ^a,b   Zeni, Luigi ^a,b   Sarti, Maurizio ^a   Sannino, Anna ^a,c   Scarfì, Maria Rosaria ^a   Vernier, P Thomas ^d   Zeni, Olga ^d  

^a CNR   (Italy)

^b SECOND UNIVERSITY OF NAPLES   (Italy)

^c UNIVERSITY OF SALERNO   (Italy)

^d University of Southern California   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL NUCLEUS DNA; DNA; COLORING AGENT; NUCLEOPROTEIN; TRYPAN BLUE;

AMPLITUDE MODULATION; ARTICLE; CELL GROWTH; CELL INTERACTION; CELL MEMBRANE PERMEABILITY; CELL NUCLEUS; CELL VIABILITY; COMET ASSAY; CONTROLLED STUDY; DNA TRANSFECTION; ELECTROPHORETIC MOBILITY; HUMAN; HUMAN CELL; INTENSE NANOSECOND PULSED ELECTRIC FIELD; LEUKEMIA CELL LINE; LYMPHOBLASTOID CELL; PULSED ELECTRIC FIELD; T LYMPHOCYTE; TRANSPORT KINETICS; CELL MEMBRANE; CELL STRAIN HL 60; CELL SURVIVAL; CHEMISTRY; ELECTROPHORESIS; GENETICS; INSTRUMENTATION; KINETICS; METABOLISM; METHODOLOGY; NANOTECHNOLOGY; PERMEABILITY; PHYSICS; PROTEIN CONFORMATION;

CELL MEMBRANE; CELL SURVIVAL; COLORING AGENTS; COMET ASSAY; DNA; ELECTROPHORESIS; HL-60 CELLS; HUMANS; JURKAT CELLS; KINETICS; NANOTECHNOLOGY; NUCLEOPROTEINS; PERMEABILITY; PHYSICS; PROTEIN CONFORMATION; TRYPAN BLUE;

EID: 82555187437     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0028419     Document Type: Article

References (32)

1. Joshi RP, Schoenbach KH, (2010) Bioelectric effects of intense ultrashort pulses. Critical Reviews in Biomedical Engineering 38: 255-304.
2. Vernier PT, Sun Y, Marcu L, Craft CM, Gundersen MA, (2004) Nanoelectropulse-Induced Phosphatidylserine Translocation, Biophys. J. 86: 4040-4048.
3. Vernier PT, Ziegler MJ, Sun Y, Gundersen MA, Tieleman P, (2006a) Nanopore-facilitated, voltage-driven phosphatidylserine translocation in lipid bilayers-in cells and in silico. Phys. Biol 3: 233-247.
4. Vernier PT, Sun Y, Gundersen MA, (2006b) Nanoelectropulse-driven membrane perturbation and small molecule permeabilization. BMC Cell Biol 7: 7-37.
5. Ibey BL, Mixon DG, Payne JA, Bowman A, Sickendick K, et al. (2010) Plasma membrane permeabilization by trains of ultrashort electric pulses. Bioelectrochemistry 79 (1): 114-121.
6. Schoenbach KH, Beebe SJ, Buescher ES, (2001) Intracellular Effect of Ultrashort Electrical Pulses. Bioelectromagnetics 22: 440-448.
7. Chen N, Schoenbach KH, Kolb JF, Swanson RJ, Garner AL, et al. (2004) Leukemic cell intracellular responses to nanosecond electric fields. Biochem Biophys Res Commun 317: 421-427.
8. Buescher ES, Schoenbach KH, (2003) Effects of Submicrosecond, High Intensity Pulsed Electric Fields on Living Cells- Intracellular Electromanipulation. IEEE Trans Dielectr and Electr Ins 5 pp. 788-794.
9. Tekle E, Oubrahim H, Dzekunov SM, Kolb JF, Schoenbach KH, et al. (2005) Selective field effects on intracellular vacuoles and vesicle membranes with nanosecond electric pulses. Biophys J 89: 274-284.
10. Vernier PT, Sun Y, Marcu L, Salemi S, Craft CM, et al. (2003) Calcium bursts induced by nanosecond electric pulses. Biochem Biophys Res Commun 310: 286-295.
11. Beebe SJ, Fox PM, Rec LJ, Somers K, Stark RH, et al. (2002) Nanosecond Pulsed Electric Field (nsPEF) Effects on Cells and Tissues: Apoptosis Induction and Tumor Growth Inhibition. IEEE Trans Plasma Sci 30 (1): 286-292.
12. Chen X, Kolb JF, Swanson RJ, Schoenbach KH, Beebe SJ, (2010) Apoptosis initiation and angiogenesis inhibition: melanoma targets for nanosecond pulsed electric fields. Pigment Cell Melanoma Res 23 (4): 554-563.
13. Nuccitelli R, Pliquett U, Chen X, Ford W, Swanson RJ, et al. (2006) Nanosecond pulsed electric fields cause melanomas to self-destruct, Biochem Biophys Res Commun 343 (2): 351-360.
14. Nuccitelli R, Chen X, Pakhomov AG, Baldwin WH, Sheikh S, et al. (2009) A new pulsed electric field therapy for melanoma disrupts the tumor's blood supply and causes complete remission without recurrence. Int J Cancer 125: 438-445.
15. Pakhomov AG, Kolb JF, White JA, Joshi RP, Xiao S, et al. (2007) Long-Lasting Plasma Membrane Permeabilization in Mammalian Cells by Nanosecond Pulsed Electric Field (nsPEF). Bioelectromagnetics 28: 655-663.
16. Pakhomov AG, Bowman AM, Ibey BL, Andre FM, Pakhomova ON, et al. (2009) Lipid nanopores can form a stable, ion channel-like conduction pathway in cell membrane. Biochem Biophys Res Commun 385: 181-186.
17. Bowman AM, Nesin ON, Pakhomova ON, Pakhomov AG, (2010) Analysis of plasma membrane integrity by fluorescent detection of Tl+ uptake. J Membr Biol 236: 5-26.
18. Vernier PT, Sun Y, Chen MT, Gundersen MA, Craviso GL, (2008) Nanosecond electric pulse-induced calcium entry into chromaffin cells. Bioelectrochemistry 73: 1-4.
19. Palumbo R, Brescia F, Capasso D, Sannino A, Sarti M, et al. (2008) Exposure to 900 MHz radiofrequency radiation induces caspase-3 activation in proliferating human lymphocytes. Radiat Res 170: 327-334.
20. Kolb JF, Kono S, Schoenbach KH, (2006) Nanosecond pulsed electric field generators for the study of subcellular effects. Bioelectromagnetics 27: 172-187.
21. Idziorek T, Estaquier J, De Bels F, Ameisen JC, (1995) YOPRO-1 permits cytofluorometric analysis of programmed cell death (apoptosis) without interfering with cell viability. J Immunol Methods 185: 249-258.
22. Singh NP, McCoy MT, Tice RR, Schneider EL, (1988) A simple technique for quantitation of low level of DNA damage in individual cells. Exp Cell Res 175: 18-187.
23. Zeni O, Scarfì MR, (2010) DNA damage by carbon nanotubes using the single cell gel electrophoresis technique. In: Balasubramanian K, Burghard M editors. Carbon Nanotubes: Methods and Protocols-Methods in Molecular Biology, Vol. 625. 109-119 New York Humana Press Inc.
24. Lovell DP, Omori T, (2008) Statistical issues in the use of the comet assay. Mutagenesis 23: 171-182.
25. Deng J, Schoenbach KH, Buescher ES, Hair PS, Fox PM, et al. (2003) The effects of intense submicrosecond electrical pulses on cells. Biophys J 84: 2709-2714.
26. Collins AR, Dusinska M, Horska A, (2001) Detection of alkylation damage in human lymphocytes DNA with the comet assay. Acta Biochim Pol 48 (3): 611-614.
27. Tice RR, Yager JW, Andrews P, Crecelius E, (1997) Effect of hepatic methyl donor status on urinary secretion and DNA damage in B6C3F1 mice treated with sodium arsenite. Mutat Res 386: 315-334.
28. Merk O, Speit G, (1999) Detection of crosslinks with the Comet assay in relationship to genotoxicity and cytotoxicity. Environ Mol Mutagen 33: 167-172.
29. Stacey M, Stickley M, Fox PM, Statler V, Schoenbach KH, et al. (2003) Differential effects in cells exposed to ultra-short high intensity electric fields: cell survival, DNA damage and cell cycle analysis. Mutat Res 542: 65-75.
30. Porschke D, Meier HJ, Ronnenberg J, (1984) Interaction of nucleic acid double helices induce by electric field pulses. Biophys Chem 20: 225-235.
31. White JA, Pliquett U, Blackmore PF, Joshi RP, Schoenbach KH, et al. (2011) Plasma membrane charging of Jurkat cells by nanosecond pulsed electric fields. Eur Biophys J 40: 947-957.
32. Matzke AJM, Matzke MA, (1991) The electrical properties of the nuclear envelope, and their possible role in the regulation of eukaryotic gene expression. Bioelectrochemistry and Bioenergetics 25: 357-370.