Optimization of fixation methods for observation of bacterial cell morphology and surface ultrastructures by atomic force microscopy

Applied Microbiology and Biotechnology

Volumn 92, Issue 2, 2011, Pages 381-392

  Chao, Yuanqing ^a   Zhang, Tong ^a  

^a UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

Atomic force microscopy; Cell morphology; Fixation methods; Ultrastructure

Indexed keywords

BACILLUS SUBTILIS; BACTERIAL CELLS; BACTERIAL SURFACES; CELL MORPHOLOGY; FIXATION METHODS; GLUTARALDEHYDES; PARAFORMALDEHYDES; PSEUDOMONAS PUTIDA; QUANTITATIVE AND QUALITATIVE ASSESSMENTS; SURFACE FILAMENTS; THREE MODELS; ULTRASTRUCTURE;

ACETAL RESINS; ATOMIC FORCE MICROSCOPY; BACTERIA; BACTERIOLOGY; CELLS; ESCHERICHIA COLI; FORMALDEHYDE; MORPHOLOGY;

SURFACE MORPHOLOGY;

ACETIC ACID; ACETONE; ALCOHOL; FORMALDEHYDE; GLUTARALDEHYDE; METHANOL; PARAFORMALDEHYDE;

ATOMIC FORCE MICROSCOPY; BACTERIUM; CYTOLOGY; OPTIMIZATION; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; ULTRASTRUCTURE;

ARTICLE; ATOMIC FORCE MICROSCOPY; BACILLUS SUBTILIS; BACTERIAL CELL; CELL STRUCTURE; ESCHERICHIA COLI; FLAGELLUM; NONHUMAN; PRESERVATION; PSEUDOMONAS PUTIDA; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; SURFACE PROPERTY; ULTRASTRUCTURE;

BACILLUS SUBTILIS; CYTOLOGICAL TECHNIQUES; ESCHERICHIA COLI; MICROSCOPY, ATOMIC FORCE; PSEUDOMONAS PUTIDA;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOMONAS PUTIDA;

EID: 82455164106     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3551-5     Document Type: Article

References (38)

1. Alsteens D, Verbelen C, Dague E, Raze D, Baulard AR, Dufrěne YF (2008) Organization of the mycobacterial cell wall: A nanoscale view. Pflugers Arch Eur J Physiol 456:117-125
2. Andre G, Kulakauskas S, Chapot-Chartier MP, Navet B, Deghorain M, Bernard E, Hols P, Dufrěne YF (2010) Imaging the nanoscale organization of peptidoglycan in living Lactococcus lactis cells. Nat Commun 1:27
3. Arce FT, Carlson R, Monds J, Veeh R, Hu FZ, Stewart PS, Lal R, Ehrlich GD, Avci R (2009) Nanoscale structural and mechanical properties of nontypeable Haemophilus influenzae biofilms. J Bacteriol 191:2512-2520
4. Auerbach ID, Sorensen C, Hansma HG, Holden PA (2000) Physical morphology and surface properties of unsaturated Pseudomonas putida biofilms. J Bacteriol 182:3809-3815 (Pubitemid 30413034)
5. Bolshakova AV, Kiselyova OI, Filonov AS, Frolova OY, Lyubchenko YL, Yaminsky IV (2001) Comparative studies of bacteria with an atomic force microscopy operating in different modes. Ultramicroscopy 86:121-128 (Pubitemid 32098262)
6. Bolshakova AV, Kiselyova OI, Yaminsky IV (2004) Microbial surfaces investigated using atomic force microscopy. Biotechnol Prog 20:1615-1622 (Pubitemid 39606442)
7. Camesano TA, Natan MJ, Logan BE (2000) Observation of changes in bacterial cell morphology using tapping mode atomic force microscopy. Langmuir 16:4563-4572
8. Celie JWAM, Beelen RHJ, van den Born J (2005) Effect of fixation protocols on in situ detection of L-selectin ligands. J Immunol Methods 298:155-159 (Pubitemid 40545101)
9. Dapson RW (2007) Macromolecular changes caused by formalin fixation and antigen retrieval. Biotech Histochem 82:133-140 (Pubitemid 350090067)
10. Dorobantu LS, Bhattacharjee S, Foght JM, Gray MR (2008) Atomic force microscopy measurement of heterogeneity in bacterial surface hydrophobicity. Langmuir 24:4944-4951 (Pubitemid 351725184)
11. Gaboriaud F, Dufrěne YF (2007) Atomic force microscopy of microbial cells: Application to nanomechanical properties, surface forces and molecular recognition forces. Colloids Surf B 54:10-19 (Pubitemid 46014412)
12. Handa H, Gurczynski S, Jackson MP, Mao G (2010) Immobilization and molecular interactions between bacteriophage and lipopolysaccharide bilayers. Langmuir 26:12095-12103
13. Harada Y, Sone H, Hosaka S (2008) Estimation of three-dimensional atomic force microscope tip shape from atomic force microscope image for accurate measurement. Jpn J Appl Phys 47:6186-6189
14. Hoetelmans RWM, Prins FA, Cornelese-ten Velde I, van der Meer J, van der Velde CJH, van Dierendonck JH (2001) Effects of acetone, methanol, or paraformaldehyde on cellular structure, visualized by reflection contrast microscopy and transmission and scanning electron microscopy. Appl Immunohistochem Mol Morphol 9:346-351 (Pubitemid 33098145)
15. Jaschke M, Butt HJ, Wolff EK (1994) Imaging flagella of Halobacteria by atomic force microscopy. Analyst 119:1943-1946 (Pubitemid 2138408)
16. Kiernan JA (2000) Formaldehyde, formalin, paraformaldehyde and glutaraldehyde: What they are and what they do. Microsc Today 00-1:8-12
17. Korhonen TK, Lounatmaa K, Ranta H, Kuusi N (1980) Characterization of type 1 pili of Salmonella typhimurium LT2. J Bacteriol 144:800-805 (Pubitemid 11164201)
18. Kuznetsov YG, McPherson A (2011) Atomic force microscopy in imaging of viruses and virus-infected cells. Microbiol Mol Biol Rev 75:268-285
19. Lomonosov AM, Egorov SN, Gallyamov MO, Yaminsky IV (2003) AFM of bacterial cells subjected to different factors. Phys Low- Dim Struct 3(4):125-130 (Pubitemid 36964064)
20. Maki-Yonekura S, Yonekura K, Namba K (2010) Conformational change of flagellin for polymorphic supercoiling of the flagellar filament. Nat Struct Mol Biol 17:417-423
21. McEwen GD, Wu YZ, Zhou AH (2010) Probing nanostructures of bacterial extracellular polymeric substances versus culture time by Raman microspectroscopy and atomic force microscopy. Biopolymers 93:171-177
22. Meade AD, Clarke C, Draux F, Sockalingum GD, Manfait M, Lyng FM, Byrne HJ (2010) Studies of chemical fixation effects in human cell lines using Raman microspectroscopy. Anal Bioanal Chem 396:1781-1791
23. Micic M, Hu D, Suh YD, Newton G, Romine M, Lu HP (2004) Correlated atomic force microscopy and fluorescence lifetime imaging of live bacterial cells. Colloids Surf B 34:205-212 (Pubitemid 38447011)
24. Moloney M, McDonnell L, O'Shea H (2004) Atomic force microscopy of BHK-21 cells: An investigation of cell fixation techniques. Ultramicroscopy 100:153-161 (Pubitemid 38834121)
25. Namba K, Vonderviszt F (1997) Molecular architecture of bacterial flagellum. Q Rev Biophys 30:1-65 (Pubitemid 27190365)
26. Paavilainen L, Edvinsson Å, Asplund A, Hober S, Kampf C, Pontén F, Wester K (2010) The impact of tissue fixatives on morphology and antibody-based protein profiling in tissues and cells. J Histochem Cytochem 58:237-246
27. Pelling AE, Li Y, Shi W, Gimzewski JK (2005) Nanoscale visualization and characterization of Myxococcus xanthus cells with atomic force microscopy. Proc Natl Acad Sci U S A 102:6484-6489 (Pubitemid 40617469)
28. Roosjen A, Boks NP, van der Mei HC, Busscher HJ, Norde W (2005) Influence of shear on microbial adhesion to PEO-brushes and glass by convective-diffusion and sedimentation in a parallel plate flow chamber. Colloids Surf B 46:1-6 (Pubitemid 41570104)
29. Sahu K, Bansal H, Mukherjee C, Sharma M, Gupta PK (2009) Atomic force microscopic study on morphological alterations induced by photodynamic action of toluidine blue O in Staphylococcus aureus and Escherichia coli. J Photochem Photobiol B 96:9-16
30. Samatey FA, Imada K, Nagashima S, Vonderviszt F, Kumasaka T, Yamamoto M, Namba K (2001) Structure of the bacterial flagellar protofilament and implications for a switch for supercoiling. Nature 410:331-337 (Pubitemid 32246042)
31. Schmid T, Burkhard J, Yeo BS, Zhang W, Zenobi R (2008) Towards chemical analysis of nanostructures in biofilms I: Imaging of biological nanostructures. Anal Bioanal Chem 391:1899-1905
32. St-Laurent J, Boulay ME, Prince P, Bissonnette E, Boulet LP (2006) Comparison of cell fixation methods of induced sputum specimens: An immunocytochemical analysis. J Immunol Methods 308:36-42 (Pubitemid 43107952)
33. Stukalov O, Korenevsky A, Beveridge TJ, Dutcher JR (2008) Use of atomic force microscopy and transmission electron microscopy for correlative studies of bacterial capsules. Appl Environ Microbiol 74:5457-5465
34. Suthipintawong C, Leong ASY, Vinyuvat S (1996) Immunostaining of cell preparations: A comparative evaluation of common fixatives and protocols. Diagn Cytopathol 15:167-174 (Pubitemid 26300045)
35. Telford JL, Barocchi MA, Margarit I, Rappuoli R, Grandi G (2006) Pili in Gram-positive pathogens. Nat Rev Microbiol 4:509-519 (Pubitemid 43905610)
36. Touhami A, Jericho MH, Boyd JM, Beveridge TJ (2006) Nanoscale characterization and determination of adhesion forces of Pseudomonas aeruginosa pili by using atomic force microscopy. J Bacteriol 188:370-377 (Pubitemid 43100524)
37. Vekemans K, Rosseel L, Wisse E, Braet F (2004) Immuno-localization of Fas and FasL in rat hepatic endothelial cells: Influence of different fixation protocols. Micron 35:303-306 (Pubitemid 38300253)
38. Young KD (2007) Bacterial morphology: Why have different shapes? Curr Opin Microbiol 10:596-600 (Pubitemid 350180588)