Microfluidics-based capture of human neutrophils for expression analysis in blood and bronchoalveolar lavage

Laboratory Investigation

Volumn 91, Issue 12, 2011, Pages 1787-1795

  Warner, Elizabeth A ^a   Kotz, Kenneth T ^b   Ungaro, Ricardo F ^a   Abouhamze, Amer S ^a   Lopez, M Cecilia ^a   Cuenca, Alex G ^a   Kelly Scumpia, Kindra M ^a   Moreno, Claudia ^a   O'Malley, Kerri A ^a   Lanz, Jennifer D ^a   Baker, Henry V ^a   Martin, Larry C ^a   Toner, Mehmet ^b   Tompkins, Ronald G ^b   Efron, Philip A ^a   Moldawer, Lyle L ^a  

^a UNIVERSITY OF FLORIDA COLLEGE OF MEDICINE   (United States)

^b MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

dextran; Ficoll; genomics; microfluidics; neutrophil isolation

Indexed keywords

DEXTRAN; FICOLL; LIPOPOLYSACCHARIDE; RNA;

ADULT RESPIRATORY DISTRESS SYNDROME; ARTICLE; BLOOD; CELL COUNT; CELL ISOLATION; CLINICAL ARTICLE; CONTROLLED STUDY; CRITICALLY ILL PATIENT; GENE EXPRESSION; GENE EXPRESSION PROFILING; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; LEUKOCYTE ACTIVATION; LUNG LAVAGE; LUNG SEQUESTRATION; MICROARRAY ANALYSIS; MICROFLUIDICS; MICROFLUIDICS CAPTURE TECHNIQUE; NEUTROPHIL; PRIORITY JOURNAL;

ACUTE LUNG INJURY; ANTIGENS, CD; BRONCHOALVEOLAR LAVAGE FLUID; CASE-CONTROL STUDIES; CELL ADHESION MOLECULES; CENTRIFUGATION, DENSITY GRADIENT; GENE EXPRESSION PROFILING; GPI-LINKED PROTEINS; HUMANS; MICROFLUIDIC ANALYTICAL TECHNIQUES; NEUTROPHILS; RESPIRATORY DISTRESS SYNDROME, ADULT; RNA;

EID: 82355184365     PISSN: 00236837     EISSN: 15300307     Source Type: Journal    
DOI: 10.1038/labinvest.2011.94     Document Type: Article

References (21)

1. Warren HS, Elson CM, Hayden DL, et al. A genomic score prognostic of outcome in trauma patients. Mol Med (Cambridge, Mass) 2009;15: 220-227.
2. Cobb JP, Moore EE, Hayden DL, et al. Validation of the riboleukogram to detect ventilator-associated pneumonia after severe injury. Ann Surg 2009;250:531-539.
3. Alizadeh AA, Eisen MB, Davis RE, et al. Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling. Nature 2000;403:503-511. (Pubitemid 30082188)
4. Chang JC, Wooten EC, Tsimelzon A, et al. Gene expression profiling for the prediction of therapeutic response to docetaxel in patients with breast cancer. Lancet 2003;362:362-369. (Pubitemid 36957839)
5. Ayers M, Symmans WF, Stec J, et al. Gene expression profiles predict complete pathologic response to neoadjuvant paclitaxel and fluorouracil, doxorubicin, and cyclophosphamide chemotherapy in breast cancer. J Clin Oncol 2004;22:2284-2293. (Pubitemid 41115384)
6. Balleine RL, Webster LR, Davis S, et al. Molecular grading of ductal carcinoma in situ of the breast. Clin Cancer Res 2008;14: 8244-8252.
7. Chung TP, Laramie JM, Meyer DJ, et al. Molecular diagnostics in sepsis: from bedside to bench. J Am Coll Surg 2006;203:585-598.
8. Feezor RJ, Baker HV, Xiao W, et al. Genomic and proteomic determinants of outcome in patients undergoing thoracoabdominal aortic aneurysm repair. J Immunol 2004;172:7103-7109. (Pubitemid 38669153)
9. Ramilo O, Allman W, Chung W, et al. Gene expression patterns in blood leukocytes discriminate patients with acute infections. Blood 2007;109:2066-2077. (Pubitemid 46348207)
10. Laudanski K, Miller-Graziano C, Xiao W, et al. Cell-specific expression and pathway analyses reveal alterations in trauma-related human T ell and monocyte pathways. Proc Natl Acad Sci USA 2006;103: 15564-15569. (Pubitemid 44625632)
11. Calvano SE, Xiao W, Richards DR, et al. A network-based analysis of systemic inflammation in humans. Nature 2005;437: 1032-1037. (Pubitemid 41486985)
12. Biberthaler P, Bogner V, Baker HV, et al. Genome-wide monocytic mRNA expression in polytrauma patients for identification of clinical outcome. Shock (Augusta, Ga) 2005;24:11-19. (Pubitemid 40946589)
13. Cobb JP, Mindrinos MN, Miller-Graziano C, et al. Application of genome-wide expression analysis to human health and disease. Proc Natl Acad Sci USA 2005;102:4801-4806. (Pubitemid 40471534)
14. Watson F, Robinson JJ, Edwards SW. Neutrophil function in whole blood and after purification: changes in receptor expression, oxidase activity and responsiveness to cytokines. Biosci Rep 1992;12:123-133.
15. Eggleton P, Gargan R, Fisher D. Rapid method for the isolation of neutrophils in high yield without the use of dextran or density gradient polymers. J Immunol Methods 1989;121:105-113. (Pubitemid 19174097)
16. Aguado MT, Pujol N, Rubiol E, et al. Separation of granulocytes from peripheral blood in a single step using discontinuous density gradients of Ficoll-Urografin. A comparative study with separation by dextran. J Immunol Methods 1980;32:41-50. (Pubitemid 10132328)
17. Nauseef WM. Methods in Molecular Biology, Vol. 412: Neutrophil Methods and Protocols, Humana Press Inc.: Totowa, NJ, 2007.
18. Sethu P, Moldawer LL, Mindrinos MN, et al. Microfluidic isolation of leukocytes from whole blood for phenotype and gene expression analysis. Anal Chem 2006;78:5453-5461. (Pubitemid 44182364)
19. Russom A, Sethu P, Irimia D, et al. Microfluidic leukocyte isolation for gene expression analysis in critically ill hospitalized patients. Clin Chem 2008;54:891-900. (Pubitemid 351671603)
20. Kotz KT, Xiao W, Miller-Graziano C, et al. Clinical microfluidics for neutrophil genomics and proteomics. Nat Med 2010;16:1042-1047.
21. Steinmetz LM, Davis RW. Maximizing the potential of functional genomics. Nat Rev 2004;5:190-201. (Pubitemid 38294548)