Efficient gene disruption in Saccharomyces cerevisiae using marker cassettes with long homologous arms prepared by the restriction-free cloning strategy

World Journal of Microbiology and Biotechnology

Volumn 27, Issue 12, 2011, Pages 2999-3003

  Zhou, Yongjin J ^a,b   Yang, Fan ^a,b   Zhang, Sufang ^a   Tan, Haidong ^a   Zhao, Zongbao K ^a  

^a DALIAN INSTITUTE OF CHEMICAL PHYSICS   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Disruption cassettes; Disruption efficiency; Gene disruption; Restriction free cloning; Saccharomyces cerevisiae

Indexed keywords

CLONING STRATEGY; DISRUPTION CASSETTES; GENE DISRUPTION; GENE DISRUPTIONS; IMPROVED METHODS; S.CEREVISIAE; SELECTION MARKER;

BINDING SITES; CLONING; EFFICIENCY; YEAST;

GENES;

FUNGI; SACCHAROMYCES CEREVISIAE;

EID: 82355164382     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11274-011-0756-9     Document Type: Article

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