A novel PCR-mediated method for one-tube generation of a gene disruption construct

Biotechnology Letters

Volumn 24, Issue 16, 2002, Pages 1307-1312

  Kuwayama, Hidekazu ^a   Obara, Shinji ^a   Morio, Takahiro ^a   Katoh, Mariko ^a   Kuhara, Satoru ^b   Tanaka, Yoshimasa ^a  

^a UNIVERSITY OF TSUKUBA   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

Author keywords

Dictyostelium discoideum; Functional analysis; Fusion PCR; Homologous recombination; Transformation

Indexed keywords

DNA FRAGMENT; EXONUCLEASE;

ARTICLE; DICTYOSTELIUM DISCOIDEUM; ENZYME INACTIVATION; GC RICH SEQUENCE; GENE AMPLIFICATION; GENE DISRUPTION; HIGH TEMPERATURE; HOMOLOGOUS RECOMBINATION; NONHUMAN; POLYMERASE CHAIN REACTION; PURIFICATION; TUBE;

DICTYOSTELIUM; DICTYOSTELIUM DISCOIDEUM; PROTOZOA;

EID: 0036671269     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1019817203917     Document Type: Article

References (7)

1. Isolation of Dictyostelium discoideum cytokinesis mutants by restriction enzyme-mediated integration of blasticidin S resistance marker
2. Precise gene disruption in Saccharomyces cerevisiae by double fusion polymerase chain reaction
3. A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae
4. PCR-mediated generation of a gene disruption construct without the use of DNA ligase and plasmid vector
5. A developmentally regulated, putative serine/threonine protein kinase is essential for development in Dictyostelium
6. PCR- and ligation-mediated synthesis of marker cassettes with long flanking homology regions for gene disruption in Saccharomyces cerevisiae
7. A transformation vector for Dicryostelium discoideum with a new selectable marker bsr