An efficient method for genomic DNA extraction from different molluscs species

International Journal of Molecular Sciences

Volumn 12, Issue 11, 2011, Pages 8086-8095

  Pereira, Jorge C ^a   Chaves, Raquel ^a   Bastos, Estela ^a   Leitão, Alexandra ^b   Guedes Pinto, Henrique ^a  

^a UNIVERSITY OF TRÁS OS MONTES AND ALTO DOURO   (Portugal)

^b INSTITUTO PORTUGUÊS DO MAR E DA ATMOSFERA   (Portugal)

Author keywords

Concentration and purity; DNA; DNA yield; Molluscs; Quickgene 810

Indexed keywords

GENOMIC DNA; DNA;

ADDUCTOR MUSCLE; AGAR GEL ELECTROPHORESIS; ANALYTICAL EQUIPMENT; ANIMAL TISSUE; ANOMIIDAE; ARTICLE; BIVALVE; CADIIDAE; CONTROLLED STUDY; DNA EXTRACTION; DNA ISOLATION; FEASIBILITY STUDY; GASTROPOD; GENETIC ANALYSIS; GENETIC PURITY; MOLECULAR WEIGHT; MOLLUSC; MURICIDAE; NONHUMAN; OYSTER; POLYMERASE CHAIN REACTION; RELIABILITY; REPRODUCIBILITY; SKELETAL MUSCLE; VENERIDAE; ANIMAL; CLASSIFICATION; DNA SEQUENCE; GENOME; GENOMICS; ISOLATION AND PURIFICATION; PROCEDURES;

ANIMALS; DNA; ELECTROPHORESIS, AGAR GEL; GENOME; GENOMICS; MOLLUSCA; SEQUENCE ANALYSIS, DNA;

EID: 82255184210     PISSN: None     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms12118086     Document Type: Article

References (22)

1. Smith, D.S.; Maxwell, P.W.; de Boer, S.H. Comparison of several methods for the extraction of DNA from potatoes and potato-derived products. J. Agric. Food Chem. 2005, 53, 9848-9859.
2. Marmiroli, N.; Peano, C.; Maestri, E. Advanced PCR Techniques in Identifying Food Components. In Food Authenticity and Traceability; Lees, M. Ed.; Woodhead Publishing: Cambridge, UK, 2003; pp. 3-33.
3. Yue, G.H.; Orban, L. Rapid isolation of DNA from fresh and preserved fish scales for polymerase chain reaction. Mar. Biotechnol. 2001, 3, 199-204.
4. Blin, N.; Stafford, D.W. A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Res. 1976, 3, 2303-2308.
5. Köchl, S.; Niederstätter, H.; Parson, W. DNA extraction and quantification of forensic samples using the phenol-chloroform method and real-time PCR. Methods Mol. Biol. 2005, 297, 13-29.
6. Miller, S.A.; Dykes, D.D.; Polesky, H.F. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res. 1988, 16, doi:10.1093/nar/16.3.1215.
7. Carter, M.J.; Milton, I.D. An inexpensive and simple method for DNA purifications on silica particles. Nucleic Acids Res. 1993, 21, doi:10.1093/nar/21.4.1044.
8. Höss, M.; Pääbo, S. DNA extraction from Pleistocene bones by a silica-based purification method. Nucleic Acids Res. 1993, 21, 3913-3914.
9. Tel-Zur, N.A.S.; Myslabodski, D.; Mizrahi, Y. Modified CTAB procedure for DNA isolation from epiphytic cacti of the genera hylocereus and selenicereus (cactaceae). Plant Mol. Biol. Rep. 1999, 17, 249-254.
10. Walsh, P.S.; Metzger, D.A.; Higuchi, R. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. Biotechniques 1991, 10, 506-513.
11. Pepinski, W.; Soltyszewski, I.; Janica, J.; Skawronska, M.; Koc-Zorawska, E. Comparison of five commercial kits for DNA extraction from human blood, saliva and muscle samples. Rocz. Akad. Med. Bialymst. 2002, 47, 270-275.
12. Winnepenninckx, B.; Backeljau, T.; de Wachter, R. Extraction of high molecular weight DNA from molluscs. Trends Genet. 1993, 9, 407.
13. Doyle, J.; Doyle, J.L. Isolation of plant DNA from fresh tissue. Focus 1990, 12, 13-15.
14. Robledo, J.A.F.; Pecher, W.T.; Vasta, G.R. High-throughput isolation of oyster DNA facilitates diagnosis of "Dermo" disease. Qiagen News 2000, 5, 15-17.
15. Popa, O.P.; Murariu, D.; Popa, L.O. Comparison of four DNA extraction methods from invasive freshwater bivalve species (Mollusca: Bivalvia) in Romanian fauna. Travaux du Muséum National d'Histoire Naturelle Grigore Antipa 2007, 6, 527-536.
16. Calderón-Cortés, N.; Quesada, M.; Cano-Camacho, H.; Zavala-Páramo, G. A simple and rapid method for DNA isolation from xylophagous insects. Int. J. Mol. Sci. 2010, 11, 5056-5064.
17. Reimann, U.; Guntermann, D.; Weber, O. High-throughput DNA purification with DNeasy 96-More than just mouse tails. Qiagen News 1998, 3, 7-9.
18. Gilbert, M.T.P.; Haselkorn, T.; Bunce, M.; Sanchez, J.J.; Lucas, S.B.; Jewell, L.D.; van Marck, E.; Worobey, M. The isolation of nucleic acids from fixed, paraffin-embedded tissues-which methods are useful when? PLoS One 2007, 2, doi:10.1371/journal.pone.0000537.
19. Santos, S.; Sa, D.; Bastos, E.; Guedes-Pinto, H.; Gut, I.; Gartner, F.; Chaves, R. An efficient protocol for genomic DNA extraction from formalin-fixed paraffin-embedded tissues. Res. Vet. Sci. 2009, 86, 421-426.
20. Joaquim, S.; Pereira, J.; Leitão, A.; Matias, D.; Chaves, R.; Guedes-Pinto, H.; Chícharo, L.; Gaspar, M.B. Genetic diversity of two Portuguese populations of the pullet carpet shell Venerupis senegalensis, based on RAPD markers: Contribution to a sustainable restocking program. Helgol. Mar. Res. 2010, 64, 289-295.
21. Pereira, J.; Chaves, R.; Leitão, A.; Matias, D.; Guedes-Pinto, H. Genetic analysis of two Portuguese populations of Ruditapes decussatus by RAPD profiling. Helgol. Mar. Res. 2011, 65, 361-367.
22. Zhang, L.; Bao, Z.; Wang, S.; Huang, X.; Hu, J. Chromosome rearrangements in Pectinidae (Bivalvia: Pteriomorphia) implied based on chromozomal localization of histone H3 gene in four scallops. Genetica 2007, 130, 193-198.