A method for quick, low-cost automated confluency measurements

Microscopy and Microanalysis

Volumn 17, Issue 6, 2011, Pages 915-922

  Topman, Gil ^a   Sharabani Yosef, Orna ^a   Gefen, Amit ^a  

^a TEL AVIV UNIVERSITY   (Israel)

Author keywords

cell culture; image analysis; microscopy; segmentation; texture; tissue engineering

Indexed keywords

ALGORITHM; ANIMAL; ARTICLE; CELL COUNT; CELL STRAIN 3T3; CULTURE TECHNIQUE; CYTOLOGY; IMAGE PROCESSING; METHODOLOGY; MOUSE; MYOBLAST; REPRODUCIBILITY; STANDARD; TISSUE ENGINEERING;

3T3-L1 CELLS; ALGORITHMS; ANIMALS; CELL COUNT; CELL CULTURE TECHNIQUES; IMAGE PROCESSING, COMPUTER-ASSISTED; MICE; MYOBLASTS; NIH 3T3 CELLS; REPRODUCIBILITY OF RESULTS; TISSUE ENGINEERING;

EID: 82055168986     PISSN: 14319276     EISSN: 14358115     Source Type: Journal    
DOI: 10.1017/S1431927611012153     Document Type: Article

References (10)

1. Ahmed, A.S., Gogal, R.M., Jr. & Walsh, J.E. (1994). A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: An alternative to @3H#thymidine incorporation assay. J Immunol Methods 170, 211-224
2. Curl, C.L., Harris, T., Harris, P.J., Allman, B.E., Bellair, C.J., Stewart, A.G. & Delbridge, L.M.D. (2004). Quantitative phase microscopy: A new tool for measurement of cell culture growth and confluency in situ. Pflugers Arch 448, 462-468
3. Ginty, P.J., Howard, D., Rose, F.R., Whitaker, M.J., Barry, J.J., Tighe, P., Mutch, S.R., Serhatkulu, G., Oreffo, R.O., Howdle, S.M. & Shakesheff, K.M. (2006). Mammalian cell survival and processing in supercritical CO(2). Proc Natl Acad Sci USA 103, 7426-7431
4. Liang, C.C., Park, A.Y. & Guan, J.L. (2007). In vitro scratch assay: A convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc 2, 329-333
5. Malpica, N., Santos, A., Tejedor, A., Torres, A., Castilla, M., Garcia-Barreno, P. & Desco, M. (2003). Automatic quantification of viability in epithelial cell cultures by texture analysis J Microsc 209, 34-40
6. Mölder, A., Sebesta, M., Gustafsson, M., Gisselson, L., Wingren, A. & Alm, K. (2008). Non-invasive, label-free cell counting and quantitative analysis of adherent cells using digital holography. J Microsc 232, 240-247
7. Or-Tzadikario, S., Sopher, R. & Gefen, A. (2010). Quantitative monitoring of lipid accumulation over time in cultured adipocytes as function of culture conditions: Toward controlled adipose tissue engineering. Tissue Eng Part C Methods 16, 1167-1181
8. Roehm, N.W., Rodgers, G.H., Hatfield, S.M. & Glasebrook, A.L. (1991). An improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT. J Immunol Methods 142, 257-265
9. 2+ concentration. Proc Natl Acad Sci USA 78, 328-332
10. Selinummi, J., Ruusuvuori, P., Podolsky, I., Ozinsky, A., Gold, E., Yli-Harja, O., Aderem, A. & Shmulevich, I. (2009). Bright field microscopy as an alternative to whole cell fluorescence in automated analysis of macrophage images. PLoS ONE 4, e7497.