Non-invasive, label-free cell counting and quantitative analysis of adherent cells using digital holography

Journal of Microscopy

Volumn 232, Issue 2, 2008, Pages 240-247

  Molder A ^a   Sebesta, M ^a   Gustafsson, M ^b   Gisselson, L ^a   Wingren, A Gjörloff ^c   Alm, K ^d  

^a Phase Holographic Imaging AB   (Sweden)

^b LUND UNIVERSITY   (Sweden)

^c MALMÖ UNIVERSITY   (Sweden)

^d LUND UNIVERSITY   (Sweden)

Author keywords

Cell analysis; Cell counting; Cell size; Cellular optical thickness; Digital holography

Indexed keywords

CELLS; HOLOGRAMS; OPTICAL PROPERTIES;

ADHERENT CELLS; CELL ANALYSIS; CELL COUNTING; CELL-SIZE; CELLULAR OPTICAL THICKNESS; CELLULARS; DIGITAL HOLOGRAPHY; FREE CELLS; LABEL FREE; OPTICAL THICKNESS;

CELL CULTURE;

ACCURACY; ADHERENT CELL; ANIMAL CELL; ARTICLE; CELL COUNT; CELL CULTURE; CELL SHAPE; CELL SIZE; CONTROLLED STUDY; HOLOGRAPHY; HUMAN; HUMAN CELL; MOUSE; NON INVASIVE MEASUREMENT; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS;

EID: 55449137792     PISSN: 00222720     EISSN: 13652818     Source Type: Journal    
DOI: 10.1111/j.1365-2818.2008.02095.x     Document Type: Article

References (19)

1. Brinkmann, M., Lütkemeyer, D., Gudermann, F. & Lehmann, J. (2002) New technologies for automated cell counting based on optical image analysis 'The Cellscreen'. Cytotechnology 38, 119 127.
2. Carl, D., Kemper, B., Wernicke, G. & von Bally, G. (2004) Parameter-optimized digital holographic microscope for high-resolution living-cell analysis. Appl. Opt. 43, 6536 6544.
3. Charrière, F., Pavillon, N., Colomb, T. et al 2006) Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba. Opt. Express 14, 7005 7013.
4. Cuche, E., P., M. & C., D. (1999) Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms. Appl. Optics 38, 6994 7001.
5. Dubois, F., Yourassowsky, C., Monnom, O. et al 2006) Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration. J. Biomed. Opt. 11, 054032.
6. Ferraro, P., Grilli, S., Alfieri, D. et al 2005) Extended focused image in microscopy by digital holography. Opt. Express 13, 6738 6749.
7. Ghiglia, D.C. & Pritt, M.D. (1998) Two-Dimensional Phase Unwrapping: Theory, Algorithms, and Software. Wiley, New York.
8. Gustafsson, M. & Sebesta, M. (2004) Refractometry of microscopic objects with digital holography. Appl. Opt. 43, 4796 4801.
9. Gustafsson, M., Sebesta, M., Bengtsson, B., Pettersson, S.G., Egelberg, P. & Lenart, T. (2004) High-resolution digital transmission microscopy - a Fourier holography approach. Opt. Lasers Eng. 553 563.
10. Hernandez, L., Gothreaux, P., Shih, L. & Campbell, G. (2006) Toward real-time biopsy image analysis and cell segmentation. Proc. Int. Conf. Image processing, computer vision and pattern recognition, 2006, Las Vegas, NV, USA, 81 87.
11. Hernandez-Montes Mdel, S., Perez-Lopez, C. & Santoyo, F.M. (2007) Finding the position of tumor inhomogeneities in a gel-like model of a human breast using 3-D pulsed digital holography. J. Biomed. Opt. 12, 024027.
12. Kemper, B., Carl, D., Schnekenburger, J., Bredebusch, I., Schafer, M., Domschke, W. & von Bally, G. (2006) Investigation of living pancreas tumor cells by digital holographic microscopy. J. Biomed. Opt. 11, 34005.
13. Mann, C.J., Yu, L., Lo, C.-M. & Kim, M.K. (2005) High-resolution quantitative phase-contrast microscopy by digital holography. Opt. Express 13, 8693 8698.
14. Marquet, P., Rappaz, B., Magistretti, P.J., Cuche, E., Emery, Y., Colomb, T. & Depeursinge, C. (2005) Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy. Opt. Lett. 30, 468 470.
15. Parshall, D. & Kim, M.K. (2006) Digital holographic microscopy with dual-wavelength phase unwrapping. Appl. Opt. 45, 451 459.
16. Rappaz, B., Marquet, P., Cuche, E., Emery, Y., Depeursinge, C. & Magistretti, P.J. (2005) Measurement of the integral refractive index and dynamic cell morphometry of living cells with digital holographic microscopy. Opt. Express 13, 9361 9373.
17. Schnars, U. & Jüptner, W.P.O. (2002) Digital recording and numerical reconstruction of holograms. Meas. Sci. Technol. 13, R85 R101.
18. Sebesta, M. & Gustafsson, M. (2005) Object characterization with refractometric digital Fourier holography. Opt. Lett. 30, 471 473.
19. Yu, P., Mustata, M., Peng, L., Turek, J.J., Melloch, M.R., French, P.M. & Nolte, D.D. (2004) Holographic optical coherence imaging of rat osteogenic sarcoma tumor spheroids. Appl. Opt. 43, 4862 4873.