Bright field microscopy as an alternative to whole cell fluorescence in automated analysis of macrophage images

PLoS ONE

Volumn 4, Issue 10, 2009, Pages

  Selinummi, Jyrki ^a,b   Ruusuvuori, Pekka ^a,b   Podolsky, Irina ^a   Ozinsky, Adrian ^a   Gold, Elizabeth ^a   Yli Harja, Olli ^b   Aderem, Alan ^a   Shmulevich, Ilya ^a,b,c,d  

^a INSTITUTE FOR SYSTEMS BIOLOGY   (United States)

^b TAMPERE UNIVERSITY OF TECHNOLOGY   (Finland)

^c University of Washington   (United States)

^d University of Washington   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; AUTOANALYSIS; BRIGHT FIELD MICROSCOPY; CELL LABELING; CELL NUCLEUS; CELL POPULATION; CELLULAR DISTRIBUTION; COMPUTER PROGRAM; CONTRAST ENHANCEMENT; CONTROLLED STUDY; FLUORESCENCE; IMAGE ANALYSIS; MACROPHAGE; MOUSE; NONHUMAN; VALIDATION PROCESS; WHOLE CELL; ALGORITHM; ANIMAL; AUTOMATED PATTERN RECOGNITION; AUTOMATION; CYTOLOGY; FLUORESCENCE MICROSCOPY; IMAGE ENHANCEMENT; IMAGE PROCESSING; METABOLISM; METHODOLOGY; MICROSCOPY; REPRODUCIBILITY; STATISTICAL MODEL;

MURINAE;

ALGORITHMS; ANIMALS; AUTOMATION; IMAGE ENHANCEMENT; IMAGE PROCESSING, COMPUTER-ASSISTED; MACROPHAGES; MICE; MICROSCOPY; MICROSCOPY, FLUORESCENCE; MODELS, STATISTICAL; PATTERN RECOGNITION, AUTOMATED; REPRODUCIBILITY OF RESULTS; SOFTWARE;

EID: 70449562096     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0007497     Document Type: Article

References (18)

1. Moffat J, Grueneberg DA, Yang X, Kim SY, Kloepfer AM, et al. (2006) A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content screen. Cell 124: 1283-1298.
2. Carpenter AE, Jones TR, Lamprecht MR, Clarke C, Kang IH, et al. (2006) CellProfiler: image analysis software for identifying and quantifying cell phenotypes. Genome Biol 7: R100.
3. Bolte S, Cordelières FP (2006) A guided tour into subcellular colocalization analysis in light microscopy. J Microsc 224: 213-232.
4. Curl CL, Bellair CJ, Harris T, Allman BE, Harris PJ, et al. (2005) Refractive index measurement in viable cells using quantitative phase-amplitude microscopy and confocal microscopy. Cytometry A 65: 88-92.
5. Ali R, Gooding M, Christlieb M, Brady M (2008) Advanced phase-based segmentation of multiple cells from brightfield microscopy images. In: Proc. 5th IEEE International Symposium on Biomedical Imaging: From Nano to Macro ISBI 2008. pp 181-184.
6. Korzynska A, Strojny W, Hoppe A, Wertheim D, Hoser P (2007) Segmentation of microscope images of living cells. Pattern Anal Appl 10: 301-319.
7. Niemistö A, Korpelainen T, Saleem R, Yli-Harja O, Aitchison J, et al. (2007) A K-means segmentation method for finding 2-D object areas based on 3-D image stacks obtained by confocal microscopy. In: Proc. 29th Annual International Conference of the IEEE Engineering in Medicine and Biology Society EMBS 2007. pp 5559-5562.
8. Gordon A, Colman-Lerner A, Chin TE, Benjamin KR, Yu RC, et al. (2007) Single-cell quantification of molecules and rates using open-source microscope-based cytometry. Nat Methods 4: 175-181.
9. Kvarnström M, Logg K, Diez A, Bodvard K, Käll M (2008) Image analysis algorithms for cell contour recognition in budding yeast. Opt Express 16: 12943-12957.
10. Zimmer C, Zhang B, Dufour A, Thebaud A, Berlemont S, et al. (2006) On the digital trail of mobile cells. IEEE Signal Proc Mag 23: 54-62.
11. Long X, Cleveland WL, Yao YL (2008) Multiclass cell detection in bright field images of cell mixtures with ECOC probability estimation. Image Vision Comput 26: 578-591.
12. Mölder A, Sebesta M, Gustafsson M, Gisselson L, Wingren AG, et al. (2008) Non-invasive, label-free cell counting and quantitative analysis of adherent cells using digital holography. J Microsc 232: 240-247.
13. Jones T, Carpenter A, Golland P (2005) Voronoi-based segmentation of cells on image manifolds. Lect Notes in Comput Sc 3765: 535-543.
14. Schlumberger MC, Käppeli R, Wetter M, Müller AJ, Misselwitz B, et al. (2007) Two newly identified SipA domains (F1, F2) steer effector protein localization and contribute to Salmonella host cell manipulation. Mol Microbiol 65: 741-760.
15. Chen TB, Lu HH, Lee YS, Lan HJ (2008) Segmentation of cDNA microarray images by kernel density estimation. J Biomed Inform 41: 1021-1027.
16. Fawcett T (2006) An introduction to ROC analysis. Pattern Recogn Lett 27: 861-874.
17. 4-synthesizing function of macrophage lipid bodies elicited by innate immune response. J Immunol 179: 8500-8508.
18. Hastie T, Friedman J, Tibshirani R (2001) The Elements of Statistical Learning: Data Mining, Inference, and Prediction. New York, NY, USA: Springer.