Optimization of a multiplex PCR assay for detecting transgenic soybean components in feed products

Applied Biochemistry and Biotechnology

Volumn 165, Issue 5-6, 2011, Pages 1225-1234

  Tian, Fang ^a,b   Wang, Xiumin ^a,c   Teng, Da ^a,c   Yang, Yalin ^a,c   Guan, Qingfeng ^a,c   Ao, Changjin ^b   Wang, Jianhua ^a,c  

^a AGRO ENVIRONMENTAL PROTECTION INSTITUTE   (China)

^b INNER MONGOLIA AGRICULTURAL UNIVERSITY   (China)

^c INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Feed; Genetically modified soybean; Multiplex PCR

Indexed keywords

35S PROMOTER; AGAROSE GEL ELECTROPHORESIS; AMPLIFIED FRAGMENTS; ANNEALING TEMPERATURES; EVENT-SPECIFIC; FEED; GENETICALLY MODIFIED SOYBEANS; MULTIPLE COMPONENTS; MULTIPLEX PCR; PRIMER SETS; SIMULTANEOUS DETECTION; TRANSGENIC SOYBEAN;

ANNEALING; ELECTROPHORESIS; GENES; MULTIPLEXING; OPTIMIZATION;

POLYMERASE CHAIN REACTION;

DECTIN 1; DECTIN 2;

AGAR GEL ELECTROPHORESIS; ARTICLE; CONCENTRATION (PARAMETERS); GENE SEQUENCE; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; PROMOTER REGION; RELIABILITY; SOYBEAN; TEMPERATURE;

ANIMAL FEED; DNA PRIMERS; MULTIPLEX POLYMERASE CHAIN REACTION; PLANTS, GENETICALLY MODIFIED; SOYBEAN PROTEINS; SOYBEANS;

GLYCINE MAX;

EID: 81955160759     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-011-9340-x     Document Type: Article

References (18)

1. EC (2003) Regulation (EC) No. 1829/2003 of the European Parliament and of the Council on genetically modified food and feed. Brussels, Belgium: European Commission.
2. Notification No. 2000-31. (2000). Ministry of Agriculture and Forestry of Korea. Seoul, Korea.
3. Notification No. 1775. (2000). Food and Marketing Bureau, Ministry of Agriculture, Forestry and Fisheries of Japan. Tokyo, Japan.
4. N Gryson K Messens K Dewettinck 2008 PCR detection of soy ingredients in bread European Food Research and Technology 227 345 351 10.1007/s00217-007-0727- 4 1:CAS:528:DC%2BD1cXlsVCltr8%3D (Pubitemid 351653747)
5. R Moriuchi K Monma N Sagi N Uno K Kamata 2007 Applicability of quantitative PCR to soy processed foods containing Roundup Ready Soy Food Control 18 191 195 10.1016/j.foodcont.2005.09.012 1:CAS:528:DC%2BD28XosVOhtbc%3D (Pubitemid 44210494)
6. I Mafra IM Ferreira MBPP Oliveira 2008 Food authentication by PCR-based methods European Food Research and Technology 227 649 665 10.1007/s00217-007- 0782-x 1:CAS:528:DC%2BD1cXmvVylt74%3D (Pubitemid 351814512)
7. VT Forte AD Pinto C Martino GM Tantillo G Grasso FP Schena 2005 A general multiplex-PCR assay for the general detection of genetically modified soya and maize Food Control l6 535 539 10.1016/j.foodcont.2004.05.010 (Pubitemid 40197681)
8. A Germini A Zanetti C Salati S Rossi C Forré S Schmid, et al. 2004 Development of a seven-target multiplex PCR for the simultaneous detection of transgenic soybean and maize in feeds and foods Journal of Agricultural and Food Chemistry 52 3275 3280 10.1021/jf035052x 1:CAS:528:DC%2BD2cXjslOhsro%3D
9. C Peano R Bordoni M Gulli A Mezzelani MC Samson D Bellis, et al. 2005 Multiplex polymerase chain reaction and ligation detection reaction/universal array technology for the traceability of genetically modified organisms in foods Analytical Biochemistry 346 90 100 10.1016/j.ab.2005.08.004 1:CAS:528:DC%2BD2MXhtFehsrzF (Pubitemid 41456630)
10. D James AM Schmidt E Wall M Green S Masri 2003 Reliable detection and identification of genetically modified maize, soybean, and canola by multiplex PCR analysis Journal of Agricultural and Food Chemistry 51 5829 5834 10.1021/jf0341159 1:CAS:528:DC%2BD3sXmsVegt7o%3D
11. M Onishi T Matsuoka T Kodama K Kashiwaba S Futo H Akiyama, et al. 2005 Development of a multiplex polymerase chain reaction method for simultaneous detection of eight events of genetically modified maize Journal of Agricultural and Food Chemistry 53 9713 9721 10.1021/jf0515476 1:CAS:528:DC%2BD2MXhtFyru7zO (Pubitemid 43027175)
12. BR Heide SM Drømtorp K Rudi E Heir AL Holck 2008 Determination of eight genetically modified maize events by quantitative, multiplex PCR and fluorescence capillary gel electrophoresis European Food Research and Technology 227 1125 1137 10.1007/s00217-008-0828-8 1:CAS:528:DC%2BD1cXotVylt7Y%3D
13. MH Zhang XJ Gao YB Yu JX AO J Qin YH Yao, et al. 2007 Detection of Roundup Ready soy in highly processed products by triplex nested PCR Food Control 18 1277 1281 10.1016/j.foodcont.2006.09.001 1:CAS:528: DC%2BD2sXkt1agsLw%3D (Pubitemid 46523865)
14. IJ Lu CH Lin TM Pan 2010 Establishment of a system based on universal multiplex-PCR for screening genetically modified crops Analytical and Bioanalytical Chemistry 396 2055 2064 10.1007/s00216-009-3214-x 1:CAS:528:DC%2BD1MXhtlWnt7vP
15. M Lipp P Brodmann K Pietsch J Pauwels E Anklam 1999 IUPAC collaborative trail study of a method to detect genetically modified soy beans and maize in dried powder Jorunal of AOAC International 82 923 928 1:CAS:528: DyaK1MXnvVamsLw%3D (Pubitemid 129560955)
16. A Chiter JM Forbes GE Blair 2000 DNA stability in plant tissues: implications for the possible transfer of genes from genetically modified food FEBS Letters 481 164 168 10.1016/S0014-5793(00)01986-4 1:CAS:528: DC%2BD3cXmtlGqsL0%3D
17. F Debode E Janssen G Berben 2007 Physical degradation of genomic DNA of soybean flours does not impair relative quantification of its transgenic content European Food Research and Technology 226 273 280 10.1007/s00217-006-0536-1 1:CAS:528:DC%2BD2sXhtVKrs7nP (Pubitemid 47460743)
18. Z Nikolić M Milošević M Vujaković D Marinković A Jevtić S Balešević-Tubić 2008 Qualitative triplex PCR for the detection of genetically modified soybean and maize Biotechnology 22 801 803