A genomic integration method for the simultaneous visualization of endogenous mRNAs and their translation products in living yeast

RNA

Volumn 17, Issue 12, 2011, Pages 2249-2255

  Haim Vilmovsky, Liora ^a   Gadir, Noga ^a   Herbst, Rebecca H ^a   Gerst, Jeffrey E ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

Author keywords

m TAG; mRNA trafficking; MS2; Protein trafficking; Yeast

Indexed keywords

ADENOSINE TRIPHOSPHATE; ADENOSINE TRIPHOSPHATE 2; FUNGAL PROTEIN; HHF2 PROTEIN; MCHERRY PROTEIN; MESSENGER RNA; MS2 RNA BINDING COAT PROTEIN; UNCLASSIFIED DRUG; VPH1 PROTEIN; YEF3 PROTEIN;

ANALYTIC METHOD; ARTICLE; BINDING SITE; GENE INSERTION; GENETIC ANALYSIS; M TAG METHOD; MITOCHONDRION; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RNA TRANSLATION; YEAST CELL;

FUNGAL PROTEINS; GENE TARGETING; GENOME, FUNGAL; HISTONES; HOMOLOGOUS RECOMBINATION; INTRACELLULAR SPACE; MEMBRANE PROTEINS; MICROSCOPY, FLUORESCENCE; MITOCHONDRIA; PEPTIDE ELONGATION FACTORS; PROTEIN BIOSYNTHESIS; PROTEIN TRANSPORT; PROTON-TRANSLOCATING ATPASES; RNA TRANSPORT; RNA, MESSENGER; YEASTS;

EID: 81755161583     PISSN: 13558382     EISSN: 14699001     Source Type: Journal    
DOI: 10.1261/rna.029637.111     Document Type: Article

References (25)

1. Andreassi C, Riccio A. 2009. To localize or not to localize: mRNA fate is in 3′UTR ends. Trends Cell Biol 19: 465-474.
2. Aronov S, Gelin-Licht R, Zipor G, Haim L, Safran E, Gerst JE. 2007. mRNAs encoding polarity and exocytosis factors are cotransported with the cortical endoplasmic reticulum to the incipient bud in Saccharomyces cerevisiae. Mol Cell Biol 27: 3441-3455. (Pubitemid 46685219)
3. Dingwall C, Laskey RA. 1991. Nuclear targeting sequences - a consensus? Trends Biochem Sci 16: 478-481.
4. Emanuelsson O, von Heijne G. 2001. Prediction of organellar targeting signals. Biochim Biophys Acta 1541: 114-119. (Pubitemid 34024833)
5. Gadir N, Haim-Vilmovsky L, Kraut-Cohen J, Gerst JE. 2011. Localization of mRNAs coding for mitochondrial proteins in the yeast Saccharomyces cerevisiae. RNA 17: 1551-1565.
6. Garcia M, Darzacq X, Delaveau T, Jourdren L, Singer RH, Jacq C. 2007. Mitochondria-associated yeast mRNAs and the biogenesis of molecular complexes. Mol Biol Cell 18: 362-368. (Pubitemid 46213207)
7. Grunstein M. 1990. Histone function in transcription. Annu Rev Cell Biol 6: 643-678.
8. Haim L, Zipor G, Aronov S, Gerst JE. 2007. A genomic integration method to visualize localization of endogenous mRNAs in living yeast. Nat Methods 4: 409-412. (Pubitemid 46901274)
9. Haim-Vilmovsky L, Gerst JE. 2009. m-TAG: a PCR-based genomic integration method to visualize the localization of specific endogenous mRNAs in vivo in yeast. Nat Protoc 4: 1274-1284.
10. Huh WK, Falvo JV, Gerke LC, Carroll AS, Howson RW, Weissman JS, O'Shea EK. 2003. Global analysis of protein localization in budding yeast. Nature 425: 686-691. (Pubitemid 37314307)
11. Jansen RP, Dowzer C, Michaelis C, Galova M, Nasmyth K. 1996. Mother cell-specific HO expression in budding yeast depends on the unconventional myosin Myo4p and other cytoplasmic proteins. Cell 84: 687-697. (Pubitemid 26094199)
12. Koch HG, Moser M, Müller M. 2003. Signal recognition particledependent protein targeting, universal to all kingdoms of life. Rev Physiol Biochem Pharmacol 146: 55-94.
13. Long RM, Singer RH, Meng X, Gonzalez I, Nasmyth K, Jansen RP. 1997. Mating type switching in yeast controlled by asymmetric localization of ASH1 mRNA. Science 277: 383-387. (Pubitemid 27450713)
14. Longtine MS, McKenzie A III, Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR. 1998. Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 14: 953-961. (Pubitemid 28328001)
15. +-ATPase. J Biol Chem 267: 14294-14303.
16. Marc P, Margeot A, Devaux F, Blugeon C, Corral-Debrinski M, Jacq C. 2002. Genome-wide analysis of mRNAs targeted to yeast mitochondria. EMBO Rep 3: 159-164. (Pubitemid 34213494)
17. Margeot A, Blugeon C, Sylvestre J, Vialette S, Jacq C, Corral-Debrinski M. 2002. In Saccharomyces cerevisiae, ATP2 mRNA sorting to the vicinity of mitochondria is essential for respiratory function. EMBO J 21: 6893-6904. (Pubitemid 36014561)
18. Oeffinger M, Wei KE, Rogers R, DeGrasse JA, Chait BT, Aitchison JD, Rout MP. 2007. Comprehensive analysis of diverse ribonucleoprotein complexes. Nat Methods 4: 951-956. (Pubitemid 350042385)
19. Qin SL, Moldave K, McLaughlin CS. 1987. Isolation of the yeast gene encoding elongation factor 3 for protein synthesis. J Biol Chem 262: 7802-7807.
20. Rose MD, Winston F, Hieter P. 1990. Methods in yeast genetics, a laboratory course manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
21. Schwartz TU. 2007. Origins and evolution of cotranslational transport to the ER. Adv Exp Med Biol 607: 52-60.
22. Smith MM, Andrésson OS. 1983. DNA sequences of yeast H3 and H4 histone genes from two non-allelic gene sets encode identical H3 and H4 proteins. J Mol Biol 169: 663-690. (Pubitemid 13007006)
23. Takeda M, Vassarotti A, Douglas MG. 1985. Nuclear genes coding the yeast mitochondrial adenosine triphosphatase complex. Primary sequence analysis of ATP2 encoding the F1-ATPase β-subunit precursor. J Biol Chem 260: 15458-15465. (Pubitemid 16170078)
24. Takizawa PA, Sil A, Swedlow JR, Herskowitz I, Vale RD. 1997. Actindependent localization of an RNA encoding a cell-fate determinant in yeast. Nature 389: 90-93. (Pubitemid 27387697)
25. Zipor G, Haim-Vilmovsky L, Gelin-Licht R, Gadir N, Brocard C, Gerst JE. 2009. Localization of mRNAs coding for peroxisomal proteins in the yeast, Saccharomyces cerevisiae. Proc Natl Acad Sci 106: 19848-19853.