m-TAG: A PCR-based genomic integration method to visualize the localization of specific endogenous mRNAs in vivo in yeast

Nature Protocols

Volumn 4, Issue 9, 2009, Pages 1274-1284

  Haim Vilmovsky, Liora ^a   Gerst, Jeffrey E ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

COAT PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; MESSENGER RNA; MS2 COAT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CLINICAL PROTOCOL; CONTROLLED STUDY; FLUORESCENCE MICROSCOPY; GENE EXPRESSION PROFILING; GENE INTERACTION; GENE LOCATION; GENETIC IDENTIFICATION; IN SITU HYBRIDIZATION; IN VIVO STUDY; MOLECULAR DYNAMICS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; RNA BINDING; SEQUENCE TAGGED SITE; SIGNAL TRANSDUCTION; YEAST;

GENOME, FUNGAL; POLYMERASE CHAIN REACTION; RNA, FUNGAL; RNA, MESSENGER; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 70249113697     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2009.115     Document Type: Article

References (19)

1. Bashirullah, A., Cooperstock, R.L. & Lipshitz, H.D. RNA localization in development. Annu. Rev. Biochem. 67, 335-394 (1998).
2. Kloc, M., Zearfoss, N.R. & Etkin, L.D. Mechanisms of subcellular mRNA localization. Cell 108, 533-544 (2002).
3. Gonsalvez, G.B., Urbinati, C.R. & Long, R.M. RNA localization in yeast: moving towards a mechanism. Biol. Cell. 97, 75-86 (2005).
4. Jansen, R.P. mRNA localization: Message on the move. Nat. Rev. Mol. Cell Biol. 2, 247-256 (2001).
5. Tekotte, H. & Davis, I. Intracellular mRNA localization: Motors move messages. Trends Genet. 18, 636-642 (2002).
6. Zenklusen, D., Larson, D.R. & Singer, R.H. Single-RNA counting reveals alternative modes of gene expression in yeast. Nat. Struct. Mol. Biol. 15, 1263-1271 (2008).
7. Beach, D.L., Salmon, E.D. & Bloom, K. Localization and anchoring of mRNA in budding yeast. Curr. Biol. 9, 569-578 (1999).
8. Bertrand, E. et al. Localization of ASH1 mRNA particles in living yeast. Mol. Cell 2, 437-445 (1998).
9. Ozawa, T., Natori, Y., Sato, M. & Umezawa, Y. Imaging dynamics of endogenous mitochondrial RNA in single living cells. Nat. Methods 4, 413-419 (2007).
10. Valencia-Burton, M., McCullough, R.M., Cantor, C.R. & Broude, N.E. RNA visualization in live bacterial cells using fluorescent protein complementation. Nat. Methods 4, 421-427 (2007).
11. Bratu, D.P., Cha, B.J., Mhlanga, M.M., Kramer, F.R. & Tyagi, S. Visualizing the distribution and transport of mRNAs in living cells. Proc. Natl. Acad. Sci. USA 100, 13308-13313 (2003).
12. Abe, H. & Kool, E.T. Flow cytometric detection of specific RNAs in native human cells with quenched autoligating FRET probes. Proc. Natl. Acad. Sci. USA 103, 263-268 (2006).
13. Haim, L., Zipor, G., Aronov, S. & Gerst, J.E. A genomic integration method to visualize localization of endogenous mRNAs in living yeast. Nat. Methods 4, 409-412 (2007).
14. Giaever, G. et al. Functional profiling of the Saccharomyces cerevisiae genome. Nature 418, 387-391 (2002).
15. Ghaemmaghami, S. et al. Global analysis of protein expression in yeast. Nature 425, 737-741 (2003).
16. Huh, W.K. et al. Global analysis of protein localization in budding yeast. Nature 425, 686-691 (2003).
17. Rose, M.D., Winston, F. & Hieter, P. Methods in Yeast Genetics, A Laboratory Course Manual (Cold Spring Harbor Press, Cold Spring Harbor, New York, 1990).
18. Brickner, D.G. et al. H2A.Z-mediated localization of genes at the nuclear periphery confers epigenetic memory of previous transcriptional state. PLoS Biol. 5, e81 (2007).
19. Straight, A.F., Belmont, A.S., Robinett, C.C. & Murray, A.W. GFP tagging of budding yeast chromosomes reveals that protein-protein interactions can mediate sister chromatid cohesion. Curr. Biol. 6, 1599-1608 (1996).