Overexpression of Kpnβ1 and Kpnα2 importin proteins in cancer derives from deregulated E2F activity

PLoS ONE

Volumn 6, Issue 11, 2011, Pages

  van der Watt, Pauline J ^a   Ngarande, Ellen ^a   Leaner, Virna D ^a  

^a UNIVERSITY OF CAPE TOWN   (South Africa)

Author keywords

[No Author keywords available]

Indexed keywords

KARYOPHERIN; KARYOPHERIN ALPHA 2; KARYOPHERIN BETA 1; PROTEIN E7; RETINOBLASTOMA PROTEIN; SMALL INTERFERING RNA; TRANSCRIPTION FACTOR DP1; TRANSCRIPTION FACTOR E2F; TRANSCRIPTION FACTOR E2F2; UNCLASSIFIED DRUG; KARYOPHERIN ALPHA; KARYOPHERIN BETA; KPNA2 PROTEIN, HUMAN; KPNB1 PROTEIN, HUMAN; LUCIFERASE;

ARTICLE; BINDING SITE; CANCER CELL CULTURE; CHROMATIN IMMUNOPRECIPITATION; CONSENSUS SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME INDUCTION; ENZYME INHIBITION; ENZYME REPRESSION; FEMALE; GENE CONSTRUCT; GENE EXPRESSION REGULATION; GENE OVEREXPRESSION; GENE SILENCING; HUMAN; HUMAN CELL; HUMAN PAPILLOMAVIRUS TYPE 16; MUTATIONAL ANALYSIS; NUCLEOTIDE SEQUENCE; PROMOTER REGION; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN FUNCTION; SITE DIRECTED MUTAGENESIS; TRANSCRIPTION INITIATION SITE; TRANSCRIPTION REGULATION; UTERINE CERVIX CANCER; CELL LINE; GENE DELETION; GENETICS; METABOLISM; NEOPLASM; PATHOLOGY; RNA INTERFERENCE; TUMOR CELL LINE; WESTERN BLOTTING;

HUMAN PAPILLOMAVIRUS;

ALPHA KARYOPHERINS; BASE SEQUENCE; BETA KARYOPHERINS; BINDING SITES; BLOTTING, WESTERN; CELL LINE; CELL LINE, TRANSFORMED; CELL LINE, TUMOR; CHROMATIN IMMUNOPRECIPITATION; E2F2 TRANSCRIPTION FACTOR; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; LUCIFERASES; MUTAGENESIS, SITE-DIRECTED; NEOPLASMS; PROMOTER REGIONS, GENETIC; PROTEIN BINDING; RETINOBLASTOMA PROTEIN; RNA INTERFERENCE; SEQUENCE DELETION; TRANSCRIPTION FACTOR DP1;

EID: 81455149500     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0027723     Document Type: Article

References (26)

1. Nakielny S, Dreyfuss G, (1999) Transport of proteins and RNAs in and out of the nucleus. Cell 23; 99 (7): 677-90.
2. Chook YM, Blobel G, (2001) Karyopherins and nuclear import. Curr Opin Struct Biol 11 (6): 703-15.
3. Kohler M, Speck C, Christiansen M, Bischoff FR, Prehn S, et al. (1999) Evidence for distinct substrate specificities of importin alpha family members in nuclear protein import. Mol Cell Biol 19 (11): 7782-91.
4. van der Watt PJ, Maske CP, Hendricks DT, Parker MI, Denny L, et al. (2009) The Karyopherin proteins, Crm1 and Karyopherin beta1, are overexpressed in cervical cancer and are critical for cancer cell survival and proliferation. Int J Cancer 124 (8): 1829-40.
5. Noetzel E, Rose M, Bornemann J, Gajewski M, Knuchel R, et al. (2011) Nuclear transport receptor karyopherin-alpha2 promotes malignant breast cancer phenotypes in vitro. Oncogene.
6. Winnepenninckx V, Lazar V, Michiels S, Dessen P, Stas M, et al. (2006) Gene expression profiling of primary cutaneous melanoma and clinical outcome. J Natl Cancer Inst 98 (7): 472-82.
7. Dahl E, Kristiansen G, Gottlob K, Klaman I, Ebner E, et al. (2006) Molecular profiling of laser-microdissected matched tumor and normal breast tissue identifies karyopherin alpha2 as a potential novel prognostic marker in breast cancer. Clin Cancer Res 12 (13): 3950-60.
8. Gluz O, Wild P, Meiler R, Diallo-Danebrock R, Ting E, et al. (2008) Nuclear karyopherin alpha2 expression predicts poor survival in patients with advanced breast cancer irrespective of treatment intensity. Int J Cancer 123 (6): 1433-8.
9. Sakai M, Sohda M, Miyazaki T, Suzuki S, Sano A, et al. (2010) Significance of karyopherin-{alpha} 2 (KPNA2) expression in esophageal squamous cell carcinoma. Anticancer Res 30 (3): 851-6.
10. Zheng M, Tang L, Huang L, Ding H, Liao WT, et al. (2010) Overexpression of karyopherin-2 in epithelial ovarian cancer and correlation with poor prognosis. Obstet Gynecol 116 (4): 884-91.
11. Wang CI, Wang CL, Wang CW, Chen CD, Wu CC, et al. (2011) Importin subunit alpha-2 is identified as a potential biomarker for non-small cell lung cancer by integration of the cancer cell secretome and tissue transcriptome. Int J Cancer 128 (10): 2364-72.
12. Mortezavi A, Hermanns T, Seifert HH, Baumgartner MK, Provenzano M, et al. (2011) KPNA2 expression is an independent adverse predictor of biochemical recurrence after radical prostatectomy. Clin Cancer Res 17 (5): 1111-21.
13. Jensen JB, Munksgaard PP, Sorensen CM, Fristrup N, Birkenkamp-Demtroder K, et al. (2011) High Expression of Karyopherin-alpha2 Defines Poor Prognosis in Non-Muscle-Invasive Bladder Cancer and in Patients with Invasive Bladder Cancer Undergoing Radical Cystectomy. Eur Urol 59 (5): 841-8.
14. Yoshitake K, Tanaka S, Mogushi K, Aihara A, Murakata A, et al. (2011) Importin-alpha1 as a Novel Prognostic Target for Hepatocellular Carcinoma. Ann Surg Oncol 18 (7): 2093-103.
15. Chen C, Wells AD, (2007) Comparative analysis of E2F family member oncogenic activity. PLoS One 2 (9): e912.
16. Muller H, Bracken AP, Vernell R, Moroni MC, Christians F, et al. (2001) E2Fs regulate the expression of genes involved in differentiation, development, proliferation, and apoptosis. Genes Dev 15 (3): 267-85.
17. Di Fiore B, Guarguaglini G, Palena A, Kerkhoven RM, Bernards R, et al. (1999) Two E2F sites control growth-regulated and cell cycle-regulated transcription of the Htf9-a/RanBP1 gene through functionally distinct mechanisms. J Biol Chem 274 (15): 10339-48.
18. Aagaard L, Lukas J, Bartkova J, Kjerulff AA, Strauss M, et al. (1995) Aberrations of p16Ink4 and retinoblastoma tumour-suppressor genes occur in distinct sub-sets of human cancer cell lines. Int J Cancer 61 (1): 115-20.
19. Bartkova J, Lukas J, Guldberg P, Alsner J, Kirkin AF, et al. (1996) The p16-cyclin D/Cdk4-pRb pathway as a functional unit frequently altered in melanoma pathogenesis. Cancer Res 56 (23): 5475-83.
20. He J, Olson JJ, James CD, (1995) Lack of p16INK4 or retinoblastoma protein (pRb), or amplification-associated overexpression of cdk4 is observed in distinct subsets of malignant glial tumors and cell lines. Cancer Res 55 (21): 4833-6.
21. Nevins JR, (2001) The Rb/E2F pathway and cancer. Hum Mol Genet 10 (7): 699-703.
22. Karstensen B, Poppelreuther S, Bonin M, Walter M, Iftner T, et al. (2006) Gene expression profiles reveal an upregulation of E2F and downregulation of interferon targets by HPV18 but no changes between keratinocytes with integrated or episomal viral genomes. Virology 353 (1): 200-9.
23. Ishida S, Huang E, Zuzan H, Spang R, Leone G, et al. (2001) Role for E2F in control of both DNA replication and mitotic functions as revealed from DNA microarray analysis. Mol Cell Biol 21 (14): 4684-99.
24. Lavia P, Jansen-Durr P, (1999) E2F target genes and cell-cycle checkpoint control. Bioessays 21 (3): 221-30.
25. Yasuhara N, Takeda E, Inoue H, Kotera I, Yoneda Y, (2004) Importin alpha/beta-mediated nuclear protein import is regulated in a cell cycle-dependent manner. Exp Cell Res 297 (1): 285-93.
26. Tang S, Tao M, McCoy JP Jr, Zheng ZM, (2006) The E7 oncoprotein is translated from spliced E6*I transcripts in high-risk human papillomavirus type 16- or type 18-positive cervical cancer cell lines via translation reinitiation. J Virol 80 (9): 4249-63.