Large-scale production of the immunomodulator c-di-GMP from GMP and ATP by an enzymatic cascade

Applied Biochemistry and Biotechnology

Volumn 165, Issue 3-4, 2011, Pages 761-775

  Spehr, Volker ^a   Warrass, Ralf ^a   Höcherl, Klaus ^b   Ilg, Thomas ^a  

^a Intervet Innovation GmbH   (Germany)

^b UNIVERSITY OF REGENSBURG   (Germany)

Author keywords

Biotechnological production; C di GMP; Diguanylate cyclase; Enzymatic cascade; Immunomodulation

Indexed keywords

ANION EXCHANGE CHROMATOGRAPHY; BIOTECHNOLOGICAL PRODUCTION; C-DI-GMP; CLINICAL STUDY; COMPOSITIONAL ANALYSIS; CYCLASES; DIGUANYLATE CYCLASE; ENZYMATIC SYNTHESIS; GUANOSINES; IMMUNOMODULATION; INTERLEUKIN-1; INTERLEUKIN-6; LARGE-SCALE PRODUCTION; MESSENGER RNA; MONOPHOSPHATES; NUCLEOSIDE-DIPHOSPHATE KINASE; ONE POT; POTENTIAL APPLICATIONS; PRODUCT INHIBITION; PURIFICATION METHOD; REVERSED-PHASE HIGH PERFORMANCE; SECOND MESSENGER; SOLVENT PRECIPITATION; THERAPEUTIC AGENTS; TUMOR NECROSIS FACTORS;

ADENOSINETRIPHOSPHATE; BIOMOLECULES; LIQUID CHROMATOGRAPHY; MACROPHAGES; MAMMALS; MASS SPECTROMETRY; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PURIFICATION; RNA; SYNTHESIS (CHEMICAL);

ENZYME INHIBITION;

(3' 5') CYCLIC DIGUANYLATE; ADENOSINE TRIPHOSPHATE; GUANOSINE PHOSPHATE; IMMUNOMODULATING AGENT; INTERLEUKIN 1BETA; INTERLEUKIN 6; LIPOPOLYSACCHARIDE; MESSENGER RNA; TUMOR NECROSIS FACTOR ALPHA; UNCLASSIFIED DRUG;

ANIMAL CELL; ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CONTROLLED STUDY; MACROPHAGE; MASS SPECTROMETRY; MOUSE; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; ONE POT SYNTHESIS; PHONETICS; REACTOR CASCADE; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

5'-GUANYLIC ACID; ADENOSINE TRIPHOSPHATE; ANIMALS; BIOTECHNOLOGY; CELL LINE; CHROMATOGRAPHY, ION EXCHANGE; CLONING, MOLECULAR; CYCLIC GMP; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GUANYLATE KINASE; IMMUNOLOGIC FACTORS; INTERLEUKIN-1BETA; INTERLEUKIN-6; LIPOPOLYSACCHARIDES; MACROPHAGES; MAGNETIC RESONANCE SPECTROSCOPY; MICE; MUTATION; NUCLEOSIDE-DIPHOSPHATE KINASE; PHOSPHORUS-OXYGEN LYASES; RECOMBINANT PROTEINS; TRANSFORMATION, BACTERIAL; TUMOR NECROSIS FACTOR-ALPHA;

ANIMALIA; BACTERIA (MICROORGANISMS); MUS;

EID: 81355138792     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-011-9294-z     Document Type: Article

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