RNase H-dependent PCR (rhPCR): Improved specificity and single nucleotide polymorphism detection using blocked cleavable primers

BMC Biotechnology

Volumn 11, Issue , 2011, Pages

  Dobosy, Joseph R ^a   Rose, Scott D ^a   Beltz, Kristin R ^a   Rupp, Susan M ^a   Powers, Kristy M ^a   Behlke, Mark A ^a   Walder, Joseph A ^a  

^a INTEGRATED DNA TECHNOLOGIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENOTYPING; HIGH SPECIFICITY; MULTIPLEX ASSAYS; RARE ALLELES; RIBONUCLEOTIDES; SINGLE NUCLEOTIDE POLYMORPHISM DETECTIONS; SINGLE NUCLEOTIDE POLYMORPHISMS; TARGET SEQUENCES;

ASSAYS; GENES; NUCLEIC ACID SEQUENCES; NUCLEOTIDES; RNA;

POLYMERASE CHAIN REACTION;

RIBONUCLEASE H; PRIMER DNA;

ALLELE; ARTICLE; BASE PAIRING; DNA SEQUENCE; ENZYME SPECIFICITY; GENE TARGETING; HYBRIDIZATION; POLYMERASE CHAIN REACTION; PYROCOCCUS ABYSSI; RNA CLEAVAGE; RNA SEQUENCE; SINGLE NUCLEOTIDE POLYMORPHISM; THERMOSTABILITY; ENZYME STABILITY; ENZYMOLOGY; GENETICS; HUMAN; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; NUCLEIC ACID HYBRIDIZATION; NUCLEOTIDE SEQUENCE; SENSITIVITY AND SPECIFICITY; STANDARD; TEMPERATURE;

PYROCOCCUS ABYSSI;

BASE SEQUENCE; DNA PRIMERS; ENZYME STABILITY; HUMANS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID HYBRIDIZATION; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE NUCLEOTIDE; PYROCOCCUS ABYSSI; RIBONUCLEASE H; SENSITIVITY AND SPECIFICITY; TEMPERATURE;

EID: 81155158408     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-11-80     Document Type: Article

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