Use of class IIS restriction enzymes for site-directed mutagenesis: Variations on phoenix mutagenesis

Analytical Biochemistry

Volumn 298, Issue 1, 2001, Pages 118-120

  Shigaki, Toshiro ^a   Hirschi, Kendal D ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

CLASS IIS RESTRICTION ENZYMES; SITE DIRECTED MUTAGENESIS;

MUTAGENESIS;

RESTRICTION ENDONUCLEASE;

AMINO ACID SEQUENCE; ARTICLE; DNA CLEAVAGE; GENE AMPLIFICATION; MOLECULAR RECOGNITION; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

PHOENIX;

EID: 0035499925     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5341     Document Type: Article

References (3)

1. Phoenix mutagenesis: One-step reassembly of multiply cleaved plasmids with mixtures of mutant and wild-type fragments
2. Expanding the potential of restriction endonucleases: Use of hapaxoterministic enzymes
3. Site-directed mutagenesis of double-stranded DNA by the polymerase chain reaction