A novel protein from the serum of Python sebae, structurally homologous with type-γ phospholipase A 2 inhibitor, displays antitumour activity

Biochemical Journal

Volumn 440, Issue 2, 2011, Pages 251-262

  Donnini, Sandra ^a   Finetti, Federica ^a   Francese, Simona ^b   Boscaro, Francesca ^c   Dani, Francesca R ^c   Maset, Fabio ^d   Frasson, Roberta ^d   Palmieri, Michele ^a   Pazzagli, Mario ^e   De Filippis, Vincenzo ^d   Garaci, Enrico ^f   Ziche, Marina ^a  

^a UNIVERSITY OF SIENA   (Italy)

^b SHEFFIELD HALLAM UNIVERSITY   (United Kingdom)

^c Geriatric Department   (Italy)

^d UNIVERSITY OF PADOVA   (Italy)

^e UNIVERSITY OF FLORENCE   (Italy)

^f UNIVERSITY OF ROME TOR VERGATA   (Italy)

Author keywords

Antitumour activity; Apoptosis; Python sebae; Snake serum; Type phospholipase inhibitor (pli )

Indexed keywords

ANTINEOPLASTIC AGENT; CYTOTOXIC AGENT; PHOSPHOLIPASE A2 INHIBITOR;

ANTINEOPLASTIC ACTIVITY; ARTICLE; CELL VIABILITY; CONTROLLED STUDY; CYTOTOXICITY; HUMAN; HUMAN CELL; IN VITRO STUDY; IN VIVO STUDY; ION EXCHANGE CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; PYTHON SEBAE; SNAKE; SQUAMOUS CELL CARCINOMA; TUMOR CELL LINE; TUMOR GROWTH; TUMOR MODEL; TUMOR XENOGRAFT;

AMINO ACID SEQUENCE; ANIMALS; ANTINEOPLASTIC AGENTS; APOPTOSIS; BOIDAE; BREAST NEOPLASMS; CARCINOMA, SQUAMOUS CELL; CELL LINE, TUMOR; CELL SURVIVAL; ENZYME INHIBITORS; FEMALE; GLIOBLASTOMA; GROUP IV PHOSPHOLIPASES A2; HUMANS; LUNG NEOPLASMS; MICE; MOLECULAR SEQUENCE DATA; PHOSPHOLIPASES A; PROTEINS; SEQUENCE ALIGNMENT;

PYTHON RETICULATUS; PYTHON SEBAE; SERPENTES;

EID: 81155123732     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20100739     Document Type: Article

References (25)

1. Thwin, M. M. and Gopalakrishnakone, P. (1998) Snake endovenomation and protective natural endogenous proteins: a mini review of the recent developments (1991-1997). Toxicon 36, 1471-1482
2. Abu-Sinna, G., Esmat, A. Y., Al-Zahaby, A. A., Soliman, N. A. and Ibrahim, T. M. (2003) Fractionation and characterization of Cerastes cerastes cerastes snake venom and the antitumor action of its lethal and non-lethal fractions. Toxicon 42, 207-215 (Pubitemid 37297550)
3. Petretski, J. H., Kanashiro, M. M., Rodrigues, F. R., Alves, E. W., Machado, O. L. and Kipnis, T. L. (2000) Edema induction by the disintegrin-like/cysteine-rich domains from a Bothrops atrox hemorrhagin. Biochem. Biophys. Res. Commun. 276, 29-34
4. Oliveira, J. C., de Oca, H. M., Duarte, M. M., Diniz, C. R. and Fortes-Dias, C. L. (2002) Toxicity of South American snake venoms measured by an in vitro cell culture assay. Toxicon 40, 321-325 (Pubitemid 33044578)
5. Schmitmeier, S., Markland, F. S. and Chen, T. C. (2000) Anti-invasive effect of contortrostatin, a snake venom disintegrin, and TNF-α on malignant glioma cells. Anticancer Res. 20, 4227-423
6. 2 enzymes. Toxicon 42, 827-840
7. Koh, D. C. I., Armugam, A. and Jeyaseelan, K. (2006) Snake venom components and their applications in biomedicine. Cell. Mol. Life Sci. 63, 3030-3304
8. 2 inhibitors from the blood plasma of the Chinese mamushi, Agkistrodon blomhoffii siniticus. Biochem. J. 325, 527-531
9. 2. Trends Pharmacol. Sci. 20, 162-170
10. 2 with the crotoxin inhibitor from Crotalus serum. Eur. J. Biochem. 267, 4799-4808
11. 2 enzymes. Biochim. Biophys. Acta 1533, 29-37
12. Ploug, M. and Ellis, V. (1994) Structure-function relationships in the receptor for urokinase-type plasminogen activator. Comparison to other members of the Ly-6 family and snake venom α-neurotoxins. FEBS Lett. 349, 163-168
13. 2 inhibitor (PLIα) from a nonvenomous snake, Elaphe quadrivirgata. IUBMB Life 55, 539-545
14. Thwin, M. M., Gopalakrishnakone, P., Kini, R. M., Armugam, A. and Jeyaseelan, K. (2000) Recombinant antitoxic and antiinflammatory factor from the nonvenomous snake Python reticulatus: phospholipase A2 inhibition and venom neutralizing potential. Biochemistry 39, 9604-9611 (Pubitemid 30626350)
15. Donnini, S., Solito, R., Monti, M., Balduini, W., Carloni, S., Cimino, M., Bampton, E. T., Pinon, L. G., Nicotera, P., Thorpe, P. E. and Ziche, M (2009) Prevention of ischemic brain injury by treatment with the membrane penetrating apoptosis inhibitor, TAT-BH4. Cell Cycle 8, 1271-1278
16. Cantara, S., Thorpe, P. E., Ziche, M. and Donnini, S. (2007) TAT-BH4 counteracts Aβ toxicity on capillary endothelium. FEBS Lett. 581, 702-706 (Pubitemid 46216296)
17. Donnini, S., Finetti, F., Solito, R., Terzuoli, E., Sacchetti, A., Morbidelli, L., Patrignani, P. and Ziche, M. (2007) EP2 prostanoid receptor promotes squamous cell carcinoma growth through epidermal growth factor receptor transactivation and iNOS and ERK1/2 pathways. FASEB J. 21, 2418-2430 (Pubitemid 47230672)
18. Moritz, R. L., Eddes, J. S., Reid, G. E. and Simpson, R. J. (1996) S-pyridylethylation of intact polyacrylamide gels and in situ digestion of electrophoretically separated proteins: a rapid mass spectrometric method for identifying cysteine-containing peptides. Electrophoresis 17, 907-917 (Pubitemid 26000785)
19. Corbett, R. J. and Roche, R. S. (1984) Use of high-speed size-exclusion chromatography for the study of protein folding and stability. Biochemistry 23, 1888-1894
20. Harding, S. E. and Jumel, K. (2001) Light scattering. In Current Protocols in Protein Science (Coligan, J. E., Dunn, B. M., Speicher, D. W. and Wingfield, P. T., eds), Chapter 7, Unit 7.8, John Wiley, New York
21. Brahms, S. and Brahms, J. (1980) Determination of protein secondary structure in solution by vacuum ultraviolet circular dichroism. J. Mol. Biol. 138, 149-178
22. Baramova, E. N., Shannon, J. D., Bjarnason, J. B. and Fox, J. W. (1989) Degradation of extracellular matrix proteins by hemorrhagic metalloproteinases. Arch. Biochem. Biophys. 275, 63-71 (Pubitemid 19280687)
23. Coelho, A. L., de Freitas, M. S., Oliveira-Carvalho, A. L., Moura-Neto, V., Zingali, R. B. and Barja-Fidalgo, C. (1999) Effects of jarastatin, a novel snake venom disintegrin, on neutrophil migration and actin cytoskeleton dynamics. Exp. Cell. Res. 251, 379-387 (Pubitemid 29431875)
24. 2 inhibitor from python serum. Eur. J. Biochem. 269, 719-727
25. 2 with antiinflammatory activity: solution structure and molecular modeling. J. Med. Chem. 50, 5938-5950