Fetal calf serum heat inactivation and lipopolysaccharide contamination influence the human T lymphoblast proteome and phosphoproteome

Proteome Science

Volumn 9, Issue , 2011, Pages

  Rahman, Hazir ^a,b   Qasim, Muhammad ^a,b   Schultze, Frank C ^a   Oellerich, Michael ^a   R Asif, Abdul ^a  

^a UNIVERSITY MEDICAL CENTER GÖTTINGEN   (Germany)

^b KOHAT UNIVERSITY OF SCIENCE AND TECHNOLOGY   (Pakistan)

Author keywords

CCRF CEM cells; FCS heat inactivation; LPS; Phosphoproteome; Proteome

Indexed keywords

BACTERIUM LIPOPOLYSACCHARIDE; CELL PROTEIN; PHOSPHOPROTEOME; PHOSPHOSPECIFIC ANTIBODY; PROTEIN ACTA; PROTEIN EIF3M; PROTEIN HYDROLYSATE; PROTEIN ICLN; PROTEIN NACA; PROTEIN PRS7; PROTEIN PSB4; PROTEIN QCR1; PROTEIN SNAPA; PROTEIN SUCB1; PROTEIN SYBF; PROTEIN TCPD; PROTEIN TCTP; PROTEOME; SILVER NITRATE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM CONTAMINATION; CELL GROWTH; COMPARATIVE STUDY; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; FETAL CALF SERUM; HEAT INACTIVATION; HEAT TREATMENT; HUMAN; HUMAN CELL; LIQUID CHROMATOGRAPHY; LYMPHATIC LEUKEMIA; NANOANALYSIS; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; SILVER STAINING; T LYMPHOBLAST; TWO DIMENSIONAL GEL ELECTROPHORESIS; UPREGULATION;

BACTERIA (MICROORGANISMS);

EID: 81055131674     PISSN: None     EISSN: 14775956     Source Type: Journal    
DOI: 10.1186/1477-5956-9-71     Document Type: Article

References (69)

1. Goustin AS, Leof EB, Shipley GD, Moses HL. Growth factors and cancer. Cancer Res 1986, 46:1015-1029.
2. Morgan JF, Morton HJ, Parker RC. Nutrition of animal cells in tissue culture; initial studies on a synthetic medium. Proc Soc Exp Biol Med 1950, 73:1-8.
3. Barta O, Nelson RA, Kuo CY. Separation of six bovine complement components and one inactivator (1, 2). Immunol Commun 1976, 5:75-86.
4. Kimura T, Miyazaki K, Mashima K, Yamashita J, Horio T, Kakuno T. Purification and properties of growth inhibitor from normal rabbit serum. J Biochem 1991, 110:423-428.
5. Otsuka H. An inhibitor present in calf serum which prevents growth of BHK21 cells in suspension culture. J Cell Sci 1972, 10:137-152.
6. Tulamo R, Frosen J, Junnikkala S, Paetau A, Kangasniemi M, Pelaez J, et al. Complement system becomes activated by the classical pathway in intracranial aneurysm walls. Lab Invest 2010, 90:168-179. 10.1038/labinvest.2009.133, 19997064.
7. Shen Y, Halperin JA, Benzaquen L, Lee CM. Characterization of neuronal cell death induced by complement activation. Brain Res Brain Res Protoc 1997, 1:186-194. 10.1016/S1385-299X(96)00026-8, 9385083.
8. Soltis RD, Hasz D, Morris MJ, Wilson ID. The effect of heat inactivation of serum on aggregation of immunoglobulins. Immunology 1979, 36:37-45. 1457381, 422227.
9. Triglia RP, Linscott WD. Titers of nine complement components, conglutinin and C3b-inactivator in adult and fetal bovine sera. Mol Immunol 1980, 17:741-748. 10.1016/0161-5890(80)90144-3, 7432351.
10. Bruinink A, Tobler U, Halg M, Grunert J. Effects of serum and serum heat-inactivation on human bone derived osteoblast progenitor cells. J Mater Sci Mater Med 2004, 15:497-501.
11. Huang FP, Stott DI. Dual inhibitory and stimulatory activities in serum from SLE patients and lupus mice that regulate the proliferation of an IL-2-dependent T cell line. Lupus 1995, 4:297-303. 10.1177/096120339500400411, 8528227.
12. Leshem B, Yogev D, Fiorentini D. Heat inactivation of fetal calf serum is not required for in vitro measurement of lymphocyte functions. J Immunol Methods 1999, 223:249-254. 10.1016/S0022-1759(98)00214-2, 10089103.
13. Khan KN, Kitajima M, Hiraki K, Fujishita A, Sekine I, Ishimaru T, et al. Toll-like receptors in innate immunity: role of bacterial endotoxin and toll-like receptor 4 in endometrium and endometriosis. Gynecol Obstet Invest 2009, 68:40-52. 10.1159/000212061, 19365133.
14. Raetz CR, Whitfield C. Lipopolysaccharide endotoxins. Annu Rev Biochem 2002, 71:635-700. 10.1146/annurev.biochem.71.110601.135414, 2569852, 12045108.
15. Xu D, Komai-Koma M, Liew FY. Expression and function of Toll-like receptor on T cells. Cell Immunol 2005, 233:85-89. 10.1016/j.cellimm.2005.04.019, 15950961.
16. Hunter T, Plowman GD. The protein kinases of budding yeast: six score and more. Trends Biochem Sci 1997, 22:18-22.
17. Nicholson-Weller A, Halperin JA. Membrane signaling by complement C5b-9, the membrane attack complex. Immunol Res 1993, 12:244-257. 10.1007/BF02918256, 8288945.
18. Yecies JL, Manning BD. Transcriptional control of cellular metabolism by mTOR signaling. Cancer Res 2011, 71:2815-2820. 10.1158/0008-5472.CAN-10-4158, 21487041.
19. Dos SS, Delattre AI, De LF, Bult H, Raes M. Gene expression profiling of LPS-stimulated murine macrophages and role of the NF-kappaB and PI3K/mTOR signaling pathways. Ann N Y Acad Sci 2007, 1096:70-77. 10.1196/annals.1397.071, 17405917.
20. Su SC, Hua KF, Lee H, Chao LK, Tan SK, Lee H, et al. LTA and LPS mediated activation of protein kinases in the regulation of inflammatory cytokines expression in macrophages. Clin Chim Acta 2006, 374:106-115. 10.1016/j.cca.2006.05.045, 16899235.
21. Zanin-Zhorov A, Tal-Lapidot G, Cahalon L, Cohen-Sfady M, Pevsner-Fischer M, Lider O, et al. Cutting edge: T cells respond to lipopolysaccharide innately via TLR4 signaling. J Immunol 2007, 179:41-44.
22. Case Gould MG. Endotoxin in Vertebrate Cell Culture: Its Measurement and Significance. In Uses and Standardization of Vertebrate Cell Lines. Tissue CultureAssociation, Gaithersburg, MD 1984, 125-136.
23. Sibley CH, Terry A, Raetz CR. Induction of kappa light chain synthesis in 70Z/3 B lymphoma cells by chemically defined lipid A precursors. J Biol Chem 1988, 263:5098-5103.
24. Epstein J, Lee MM, Kelly CE, Donahoe PK. Effect of E. coli endotoxin on mammalian cell growth and recombinant protein production. In Vitro Cell Dev Biol 1990, 26:1121-1122. 10.1007/BF02623686, 2079460.
25. Sutmuller RP, den Brok MH, Kramer M, Bennink EJ, Toonen LW, Kullberg BJ, et al. Toll-like receptor 2 controls expansion and function of regulatory T cells. J Clin Invest 2006, 116:485-494. 10.1172/JCI25439, 1332026, 16424940.
26. Wille JJ, Park J, Elgavish A. Effects of growth factors, hormones, bacterial lipopolysaccharides, and lipotechoic acids on the clonal growth of normal ureteral epithelial cells in serum-free culture. J Cell Physiol 1992, 150:52-58. 10.1002/jcp.1041500108, 1730786.
27. Gorbet MB, Sefton MV. Endotoxin: the uninvited guest. Biomaterials 2005, 26:6811-6817. 10.1016/j.biomaterials.2005.04.063, 16019062.
28. Gerharz CD, Gabbert HE, Biesalski HK, Engers R, Luley C. Fetal calf serum and retinoic acid affect proliferation and terminal differentiation of a rat rhabdomyosarcoma cell line (BA-HAN-1C). Br J Cancer 1989, 59:61-67. 10.1038/bjc.1989.12, 2246961, 2757924.
29. Freshney RI. Culture of animal cells. A manual of basic technique. Wiley-Liss, New York 1994, 190-191.
30. Li QG, Blacher R, Esch F, Congote LF. Isolation from fetal bovine serum of an apolipoprotein-H-like protein which inhibits thymidine incorporation in fetal calf erythroid cells. Biochem J 1990, 267:261-264. 1131275, 2327984.
31. Kabat EA, Mayer MM. Expeimental Immunochemsitry. Charles C Thomas, Springfield, Illinois 1961,
32. Manor E. Human plasma accelerates immortalization of B lymphocytes by Epstein-Barr virus. Cell Prolif 2008, 41:292-298. 10.1111/j.1365-2184.2008.00513.x, 18336473.
33. Tuyaerts S, Noppe SM, Corthals J, Breckpot K, Heirman C, de GC, et al. Generation of large numbers of dendritic cells in a closed system using Cell Factories. J Immunol Methods 2002, 264:135-151. 10.1016/S0022-1759(02)00099-6, 12191517.
34. Bollard CM, Aguilar L, Straathof KC, Gahn B, Huls MH, Rousseau A, et al. Cytotoxic T lymphocyte therapy for Epstein-Barr virus+ Hodgkin's disease. J Exp Med 2004, 200:1623-1633. 10.1084/jem.20040890, 2211993, 15611290.
35. Ayache S, Panelli MC, Byrne KM, Slezak S, Leitman SF, Marincola FM, et al. Comparison of proteomic profiles of serum, plasma, and modified media supplements used for cell culture and expansion. J Transl Med 2006, 4:40. 10.1186/1479-5876-4-40, 1601968, 17020621.
36. Kuznetsov SA, Mankani MH, Robey PG. Effect of serum on human bone marrow stromal cells: ex vivo expansion and in vivo bone formation. Transplantation 2000, 70:1780-1787. 10.1097/00007890-200012270-00018, 11152111.
37. Liu X, Liang J, Li G. Lipopolysaccharide promotes adhesion and invasion of hepatoma cell lines HepG2 and HepG2.2.15. Mol Biol Rep 2010, 37:2235-2239. 10.1007/s11033-009-9710-4, 19680784.
38. Hsu RY, Chan CH, Spicer JD, Rousseau MC, Giannias B, Rousseau S, et al. LPS-induced TLR4 signaling in human colorectal cancer cells increases beta1 integrin-mediated cell adhesion and liver metastasis. Cancer Res 2011, 71:1989-1998. 10.1158/0008-5472.CAN-10-2833, 21363926.
39. He W, Liu Q, Wang L, Chen W, Li N, Cao X. TLR4 signaling promotes immune escape of human lung cancer cells by inducing immunosuppressive cytokines and apoptosis resistance. Mol Immunol 2007, 44:2850-2859. 10.1016/j.molimm.2007.01.022, 17328955.
40. Kolupaeva VG, Unbehaun A, Lomakin IB, Hellen CU, Pestova TV. Binding of eukaryotic initiation factor 3 to ribosomal 40S subunits and its role in ribosomal dissociation and anti-association. RNA 2005, 11:470-486. 10.1261/rna.7215305, 1370736, 15703437.
41. Turk B. Targeting proteases: successes, failures and future prospects. Nat Rev Drug Discov 2006, 5:785-799. 10.1038/nrd2092, 16955069.
42. Dhungana S, Merrick BA, Tomer KB, Fessler MB. Quantitative proteomics analysis of macrophage rafts reveals compartmentalized activation of the proteasome and of proteasome-mediated ERK activation in response to lipopolysaccharide. Mol Cell Proteomics 2009, 8:201-213. 10.1074/mcp.M800286-MCP200, 2621002, 18815123.
43. Bommer UA, Thiele BJ. The translationally controlled tumour protein (TCTP). Int J Biochem Cell Biol 2004, 36:379-385. 10.1016/S1357-2725(03)00213-9, 14687915.
44. Yarm FR. Plk phosphorylation regulates the microtubule-stabilizing protein TCTP. Mol Cell Biol 2002, 22:6209-6221. 10.1128/MCB.22.17.6209-6221.2002, 134017, 12167714.
45. Blitvich BJ, Rayms-Keller A, Blair CD, Beaty BJ. Complete cDNA and deduced amino acid sequence of the chaperonin containing T-complex polypeptide 1 (CCT) delta subunit from Aedes triseriatus mosquitoes. DNA Seq 2001, 12:203-208.
46. Zilkha-Falb R, Barzilai A, Djaldeti R, Ziv I, Melamed E, Shirvan A. Involvement of T-complex protein-1delta in dopamine triggered apoptosis in chick embryo sympathetic neurons. J Biol Chem 2000, 275:36380-36387. 10.1074/jbc.M001692200, 10954701.
47. Kim SH, Shim KS, Lubec G. Human brain nascent polypeptide-associated complex alpha subunit is decreased in patients with Alzheimer' s disease and Down syndrome. J Investig Med 2002, 50:293-301. 10.2310/6650.2002.33287, 12109594.
48. Bursten SL, Stevenson F, Torrano F, Lovett DH. Mesangial cell activation by bacterial endotoxin. Induction of rapid cytoskeletal reorganization and gene expression. Am J Pathol 1991, 139:371-382. 1886077, 1867323.
49. Shirai Y, Sasaki N, Kishi Y, Izumi A, Itoh K, Sameshima M, et al. Regulation of levels of actin threonine phosphorylation during life cycle of Physarum polycephalum. Cell Motil Cytoskeleton 2006, 63:77-87. 10.1002/cm.20110, 16374832.
50. Pu WT, Krapivinsky GB, Krapivinsky L, Clapham DE. pICln inhibits snRNP biogenesis by binding core spliceosomal proteins. Mol Cell Biol 1999, 19:4113-4120. 104370, 10330151.
51. Hagopian JC, Reis M, Kitajima JP, Bhattacharya D, de Oliveira MC. Comparative analysis of the complete plastid genome sequence of the red alga Gracilaria tenuistipitata var. liui provides insights into the evolution of rhodoplasts and their relationship to other plastids. J Mol Evol 2004, 59:464-477. 10.1007/s00239-004-2638-3, 15638458.
52. Schagger H, Brandt U, Gencic S, von JG. Ubiquinol-cytochrome-c reductase from human and bovine mitochondria. Methods Enzymol 1995, 260:82-96.
53. Ostergaard E. Disorders caused by deficiency of succinate-CoA ligase. Journal of Inherited Metabolic Disease 2008, 31:226-229.
54. Hamilton TA, Jansen MM, Somers SD, Adams DO. Effects of bacterial lipopolysaccharide on protein synthesis in murine peritoneal macrophages: relationship to activation for macrophage tumoricidal function. J Cell Physiol 1986, 128:9-17. 10.1002/jcp.1041280103, 3087999.
55. Stott DI, Williamson AR. Non-histone chromatin proteins of B lymphocytes stimulated by lipopolysaccharide. Biochim Biophys Acta 1978, 521:726-738.
56. Stott DI. Non-histone chromatin proteins of B lymphocytes stimulated by lipopolysaccharide. III. De novo synthesis. Biochim Biophys Acta 1980, 610:371-383.
57. Vogel SN, Hilfiker ML, Caulfield MJ. Endotoxin-induced T lymphocyte proliferation. J Immunol 1983, 130:1774-1779.
58. Hinderlich S, Berger M, Schwarzkopf M, Effertz K, Reutter W. Molecular cloning and characterization of murine and human N-acetylglucosamine kinase. Eur J Biochem 2000, 267:3301-3308. 10.1046/j.1432-1327.2000.01360.x, 10824116.
59. Srinivasula SM, Datta P, Fan XJ, Fernandes-Alnemri T, Huang Z, Alnemri ES. Molecular determinants of the caspase-promoting activity of Smac/DIABLO and its role in the death receptor pathway. J Biol Chem 2000, 275:36152-36157. 10.1074/jbc.C000533200, 10950947.
60. Devroe E, Erdjument-Bromage H, Tempst P, Silver PA. Human Mob proteins regulate the NDR1 and NDR2 serine-threonine kinases. J Biol Chem 2004, 279:24444-24451. 10.1074/jbc.M401999200, 15067004.
61. MacMillan-Crow LA, Cruthirds DL. Invited review: manganese superoxide dismutase in disease. Free Radic Res 2001, 34:325-336. 10.1080/10715760100300281, 11328670.
62. Grigolo B, Borzi RM, Mariani E, Monaco MC, Cattini L, Porstmann T, et al. Intracellular Cu/Zn superoxide dismutase levels in T and non-T cells from normal aged subjects. Mech Ageing Dev 1994, 73:27-37. 10.1016/0047-6374(94)90035-3, 8028395.
63. Tian L, White JE, Lin HY, Haran VS, Sacco J, Chikkappa G, et al. Induction of Mn SOD in human monocytes without inflammatory cytokine production by a mutant endotoxin. Am J Physiol 1998, 275:C740-C747.
64. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72:248-254. 10.1016/0003-2697(76)90527-3, 942051.
65. Gorg A, Obermaier C, Boguth G, Harder A, Scheibe B, Wildgruber R, et al. The current state of two-dimensional electrophoresis with immobilized pH gradients. Electrophoresis 2000, 21:1037-1053. 10.1002/(SICI)1522-2683(20000401)21:6<1037::AID-ELPS1037>3.0.CO;2-V, 10786879.
66. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685. 10.1038/227680a0, 5432063.
67. Blum H, . BHGHJ Improved silver staining of plant-proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 1987, 8:93-99. BHGHJ.
68. Shevchenko A, Wilm M, Vorm O, Mann M. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal Chem 1996, 68:850-858. 10.1021/ac950914h, 8779443.
69. Asif AR, Oellerich M, Amstrong VW, Riemenschneider B, Monod M, Reichard U. Proteome of conidial surface associated proteins of Aspergillus fumigatus reflecting potential vaccine candidates and allergens. J Proteome Res 2006, 5:954-962. 10.1021/pr0504586, 16602703.