Development and inter-laboratory validation study of an improved new real-time PCR assay with internal control for detection and laboratory diagnosis of African swine fever virus

Journal of Virological Methods

Volumn 178, Issue 1-2, 2011, Pages 161-170

  Tignon, Marylène ^a   Gallardo, Carmina ^b   Iscaro, Carmen ^c   Hutet, Evelyne ^d   Van der Stede, Yves ^a   Kolbasov, Denis ^e   De Mia, Gian Mario ^c   Le Potier, Marie Frédérique ^d   Bishop, Richard P ^f   Arias, Marisa ^b   Koenen, Frank ^a  

^a VETERINARY AND AGROCHEMICAL RESEARCH CENTRE   (Belgium)

^b Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria Consejo Superior de Investigaciones Científicas   (Spain)

^c ISTITUTO ZOOPROFILATTICO SPERIMENTALE DELL UMBRIA E DELLE MARCHE   (Italy)

^d Swine Immunology and Virology Unit   (France)

^e Federal Agency of Scientific Organizations   (Russian Federation)

^f INTERNATIONAL LIVESTOCK RESEARCH INSTITUTE   (Kenya)

Author keywords

African swine fever virus (ASFV); Diagnosis; Internal endogenous control; Real time PCR assay; Validation

Indexed keywords

BETA ACTIN;

AFRICAN SWINE FEVER VIRUS; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; GENE; GENOTYPE; INTERMETHOD COMPARISON; LABORATORY DIAGNOSIS; LIMIT OF DETECTION; NONHUMAN; P27 GENE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VALIDATION STUDY; VALIDITY; VIRUS DETECTION; VIRUS GENOME; VIRUS INFECTION; VIRUS ISOLATION; VIRUS VIRULENCE;

ACTINS; AFRICAN SWINE FEVER; AFRICAN SWINE FEVER VIRUS; ANIMALS; CAPSID PROTEINS; CLINICAL LABORATORY TECHNIQUES; DNA PRIMERS; DNA, VIRAL; EUROPEAN UNION; REAL-TIME POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SWINE; VETERINARY MEDICINE; VIROLOGY;

AFRICAN SWINE FEVER VIRUS; SUIDAE; SUS SCROFA;

EID: 80955145757     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2011.09.007     Document Type: Article

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