Development and validation of a duplex real-time PCR assay for the simultaneous detection and quantification of porcine circovirus type 2 and an internal control on porcine semen samples

Journal of Virological Methods

Volumn 149, Issue 2, 2008, Pages 217-225

  Pal, N ^a   Huang, Y W ^b   Madson, D M ^a   Kuster, C ^c   Meng, X J ^b   Halbur, P G ^a   Opriessnig, T ^a  

^a IOWA STATE UNIVERSITY   (United States)

^b CENTER FOR MOLECULAR MEDICINE AND INFECTIOUS DISEASES   (United States)

^c Kuster Research and Consulting   (United States)

Author keywords

Internal control; PCV2; Porcine circovirus type 2; Quantitative PCR; Semen

Indexed keywords

ARTICLE; CELL FRACTIONATION; CIRCOVIRIDAE; CONTROLLED STUDY; DNA EXTRACTION; ENZYME LINKED IMMUNOSORBENT ASSAY; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SEMEN ANALYSIS; SENSITIVITY AND SPECIFICITY; SWINE; VALIDATION STUDY;

ANIMALS; CIRCOVIRIDAE INFECTIONS; CIRCOVIRUS; DNA PRIMERS; MALE; PLASMIDS; POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; REPRODUCIBILITY OF RESULTS; SEMEN; SENSITIVITY AND SPECIFICITY; SWINE; SWINE DISEASES;

PORCINE CIRCOVIRUS 2; SUS;

EID: 41549154099     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2008.02.002     Document Type: Article

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