Uniform accumulation of recombinant miraculin protein in transgenic tomato fruit using a fruit-ripening-specific E8 promoter

Transgenic Research

Volumn 20, Issue 6, 2011, Pages 1285-1292

  Hirai, Tadayoshi ^a   Kim, You Wang ^a   Kato, Kazuhisa ^a   Hiwasa Tanase, Kyoko ^a   Ezura, Hiroshi ^a  

^a UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

E8 promoter; Miraculin; Taste modifying protein; Tomato

Indexed keywords

GLYCOPROTEIN; MESSENGER RNA; MIRACULIN PROTEIN, SYNSEPALUM DULCIFICUM; RECOMBINANT PROTEIN;

ARTICLE; CAULIFLOWER MOSAIC VIRUS; COMPARATIVE STUDY; FRUIT; GENE EXPRESSION REGULATION; GENE VECTOR; GENETIC TRANSFORMATION; GENETICS; METABOLISM; PIGMENTATION; PROMOTER REGION; RHIZOBIUM; SYNSEPALUM; TOMATO; TRANSGENIC PLANT;

AGROBACTERIUM; CAULIMOVIRUS; FRUIT; GENE EXPRESSION REGULATION, PLANT; GENETIC VECTORS; GLYCOPROTEINS; LYCOPERSICON ESCULENTUM; PIGMENTATION; PLANTS, GENETICALLY MODIFIED; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; RNA, MESSENGER; SYNSEPALUM; TRANSFORMATION, GENETIC;

LYCOPERSICON ESCULENTUM;

EID: 80855144750     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11248-011-9495-9     Document Type: Article

References (40)

1. Benfey PN, Ellis J, Pelletier G (1990) Tissue-specific expression from CaMV 35S enhancer subdomains in early stages of plant development. EMBO J 9: 1677-1684.
2. De La Garza RD, Quinlivan EP, Klaus SMJ et al (2004) Folate biofortification in tomatoes by engineering the pteridine branch of folate synthesis. Proc Natl Acad Sci USA 101: 13720-13725.
3. Deblaere R, Bytebier B, De greve H, Deboeck FSJ, van Montagu M, Leemans J (1985) Efficient octopine Ti plasmid-derived vectors for Agrobacterium-mediated gene transfer to plants. Nucl Acid Res 13: 4777-4788.
4. Deikman J, Fischer RL (1988) Interaction of a DNA binding factor with the 5¢-flanking region of an ethylene-responsive fruit ripening gene from tomato. EMBO J 7: 3315-3320.
5. Deikman J, Kline R, Fischer RL (1992) Organization of ripening and ethylene regulatory regions in a fruit-specific promoter from tomato (Lycopersicon esculentum). Plant Physiol 100: 2013-2017.
6. Deikman J, Xu R, Kneissl ML, Ciardi JA, Kim KN, Pelah D (1998) Separation of cis elements responsive to elements responsive to ethylene, ethylene, fruit development, and ripening in the 5′-flanking region of the ripening-related E8 gene. Plant Mol Biol 37: 1001-1011.
7. Dellapenna D, Lincoln JE, Fischer RL, Bennett AB (1989) Transcriptional analysis of polygalacturonase and other ripening associated genes in rutgers, rin, nor, and Nr tomato fruit. Plant Physiol 90: 1372-1377.
8. Estornell LH, Orzaez D, Lopez-Pena L, Pineda B, Anton MT, Moreno V, Granell A (2009) A multisite gateway-based toolkit for targeted gene expression and hairpin RNA silencing in tomato fruits. Plant Biotechnol J 7: 298-309.
9. Fluhr R, Kuhlemeier C, Nagy F, Chua NH (1986) Organ-specific and light-induced expression of plant genes. Science 232: 1106-1112.
10. Giovannoni JJ, DellaPenna D, Bennett AB, Fischer RL (1989) Expression of a chimeric polygalacturonase gene in transgenic rin (ripening inhibitor) tomato fruit results in polyuronide degradation but not fruit softening. Plant Cell 1: 53-63.
11. Good X, Kellogg JA, Wagoner W, Langhoff D, Matsumura W, Bestwick RK (1994) Reduced ethylene synthesis by transgenic tomatoes expressing S-adenosylmethionine hydrolase. Plant Mol Biol 26: 781-790.
12. He ZM, Jiang XL, Qi Y, Luo DQ (2008) Assessment of the utility of the tomato fruit-specific E8 promoter for driving vaccine antigen expression. Genetica 133: 207-214.
13. Hirai T, Sato M, Toyooka K, Sun HJ, Yano M, Ezura H (2010) Miraculin, a taste-modifying protein is secreted into intercellular spaces in plant cells. J Plant Physiol 167: 209-215.
14. Hiwasa-Tanase K, Nyarubona M, Hirai T, Kato K, Ichikawa T, Ezura H (2011) High-level accumulation of recombinant miraculin protein in transgenic tomatoes expressing a synthetic miraculin gene with optimized codon usage terminated by the native miraculin terminator. Plant Cell Rep 30: 113-124.
15. Jiang XL, He ZM, Peng ZQ, Qi Y, Chen Q, Yu SY (2007) Cholera toxin B protein in transgenic tomato fruit induces systemic immune response in mice. Transgenic Res 16: 169-175.
16. Kato K, Yoshida R, Kikuzaki A, Hirai T, Kuroda H, Hiwasa-Tanase K, Takane K, Ezura H, Mizoguchi T (2010) Molecular breeding of tomato lines for mass production of miraculin in a plant factory. J Agric Food Chem 58: 9505-9510.
17. Kaulen H, Schell J, Kreuzaler F (1986) Light-induced expression of the chimeric chalcone synthase-NPTII gene in tobacco cells. EMBO J 5: 1-8.
18. Kim YW, Kato K, Hirai T, Hiwasa-Tanase K, Ezura H (2010) Spatial and developmental profiling of miraculin accumulation in transgenic tomato fruits expressing the miraculin gene constitutively. J Agric Food Chem 58: 282-286.
19. Lewinsohn E, Schalechet F, Wilkinson J, Matsui K, Tadmor Y, Nam KH, Amar O, Lastochkin E, Larkov O, Ravid U, Hiatt W, Gepstein S, Pichersky E (2001) Enhanced levels of the aroma and flavor compound S-linalool by metabolic engineering of the terpenoid pathway in tomato fruits. Plant Physiol 127: 1256-1265.
20. Lincoln JE, Fischer RL (1988a) Diverse mechanisms for the regulation of ethylene-inducible gene expression. Mol Gen Genet 212: 71-75.
21. Lincoln JE, Fischer RL (1988b) Regulation of gene expression by ethylene in wild-type and rin tomato (Lycopersicon esculentum) fruit. Plant Physiol 88: 370-374.
22. Lincoln JE, Cordes S, Read E, Fischer RL (1987) Regulation of gene expression by ethylene during Lycopersicon esculentum (tomato) fruit development. Proc Natl Acad Sci USA 84: 2793-2797.
23. Mehta RA, Cassol T, Li N, Ali N, Handa AK, Mattoo AK (2002) Engineered polyamine accumulation in tomato enhances phytonutrient content, juice quality, and vine life. Nat Biotechnol 20: 613-618.
24. Nagaya S, Kawamura K, Shinmyo A, Kato K (2010) The HSP terminator of Arabidopsis thaliana increase gene expression in plant cells. Plant Cell Physiol 51: 328-332.
25. Ramirez YJP, Tasciotti E, Gutierrez-Ortega A, Donayre AJ, Flores MTO, Giacca M, Lim MAG (2007) Fruit-specific expression of the human immunodeficiency virus type 1 Tat gene in tomato plants and its immunogenic potential in mice. Clin Vaccine Immunol 14: 685-692.
26. Sandhu JS, Rasnyanski SF, Domier LL, Korban SS, Osadjan MD, Buetow DE (2000) Oral immunisation of mice with transgenic tomato fruit expressing respiratory syncytial virus-F protein induces a systemic immune response. Trans Res 9: 127-135.
27. Satoh J, Kato K, Shinmyo A (2004) The 5′-untranslated region of tobacco alcohol dehydrogenase gene functions as an effective translational enhancer in plant. J Biosci Bioeng 1: 1-8.
28. Stougaard J, Sandal NN, Gron A, Kuhle A, Marcker KA (1987) 5′ Analysis of the soybean leghaemoglobin lbc3 gene: regulatory elements required for promoter activity and organ specificity. EMBO J 6: 3565-3569.
29. Sugaya T, Yano M, Sun HJ, Hirai T, Ezura H (2008) Transgenic strawberry expressing a taste-modifying protein, miraculin. Plant Biotechnol 25: 329-333.
30. Sun HJ, Cui ML, Ma B, Ezura H (2006a) Functional expression of the taste-modifying protein, miraculin, in transgenic lettuce. FEBS Lett 580: 620-626.
31. Sun HJ, Uchii S, Watanabe S, Ezura H (2006b) A highly efficient transformation protocol for Micro-Tom, a model cultivar of tomato functional genomics. Plant Cell Physiol 47: 426-431.
32. Sun HJ, Kataoka H, Yano M, Ezura H (2007) Genetically stable expression of functional miraculin, a new type of alternative sweetener, in transgenic tomato plants. Plant Biotechnol J 5: 768-777.
33. Sunilkumar G, Mohr L, Lopata-Finch E, Emani C, Kathore KS (2002) Developmental and tissue-specific expres sion of a CaMV 35S promoter in cotton as revealed by GFP. Plant Mol Biol 50: 463-474.
34. Szwacka M, Siedlecka E, Zawirska-Wojtasiak R, Wisniewski L, Malepszy S (2009) Expression pattern of the pre-prothaumatin II gene under the control of the CaMV 35S promoter in transgenic cucumber (Cucumis sativus L.) flower buds and fruits. J Appl Genet 50: 9-16.
35. Theerasilp S, Kurihara Y (1988) Complete purification and characterization of the taste-modifying protein, miraculin, from miracle fruit. J Biol Chem 263: 11536-11539.
36. Theologis A, Oeller PW, Wong LM, Rottmann WH, Gantz DM (1993) Use of a tomato mutant constructed with reverse genetics to study fruit ripening, a complex developmental process. Dev Genet 14: 282-295.
37. van Haaren MJJ, Houck CM (1993) A functional map of the fruit specific promoter of the tomato 2A11 gene. Plant Mol Biol 21: 625-640.
38. Williamson JD, Hirsch-Wyncott ME, Larkins BA, Gelvin SB (1989) Differential accumulation of a transcript driven by the CaMV 35S promoter in transgenic tobacco. Plant Physio 90: 1570-1576.
39. Xu R, Goldman S, Coupe S, Deikman J (1996) Ethylene control of E4 transcription during tomato fruit ripening involves two cooperativecis elements. Plant Mol Biol 31: 1117-1127.
40. Yano M, Hirai T, Kato K, Hiwasa-Tanase K, Fukuda N, Ezura H (2010) Tomato is a suitable material for producing recombinant miraculin protein in genetically stable manner. Plant Sci 178: 469-473.