Physiological and histological impact of Azadirachta indica (neem) leaves extract in a rat model of cisplatin-induced hepato and nephrotoxicity

Journal of Medicinal Plant Research

Volumn 5, Issue 23, 2011, Pages 5499-5506

  Ezz Din, Doaa ^a   Gabry, Mohamed S ^a   Farrag, Abdel Razik H ^b   Abdel Moneim, Ahmed E ^a  

^a Helwan University   (Egypt)

^b NATIONAL RESEARCH CENTRE   (Egypt)

Author keywords

Azadirachta indica; Cisplatin; Kidney; Liver; Rats

Indexed keywords

ALANINE AMINOTRANSFERASE; ALKALINE PHOSPHATASE; ASPARTATE AMINOTRANSFERASE; AZADIRACHTA INDICA EXTRACT; BILIRUBIN; CREATININE; GAMMA GLUTAMYLTRANSFERASE; UREA; URIC ACID;

ALANINE AMINOTRANSFERASE BLOOD LEVEL; ALKALINE PHOSPHATASE BLOOD LEVEL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; ASPARTATE AMINOTRANSFERASE BLOOD LEVEL; BILIRUBIN BLOOD LEVEL; CONTROLLED STUDY; CREATININE BLOOD LEVEL; DRUG EFFECT; DRUG MECHANISM; GAMMA GLUTAMYL TRANSFERASE BLOOD LEVEL; HISTOLOGY; LIVER PROTECTION; LIVER TOXICITY; MALE; NEPHROTOXICITY; NONHUMAN; PLANT PHYSIOLOGY; RAT; RENAL PROTECTION; UREA BLOOD LEVEL; URIC ACID BLOOD LEVEL;

AZADIRACHTA INDICA; RATTUS;

EID: 80755125324     PISSN: None     EISSN: 19960875     Source Type: Journal    
DOI: None     Document Type: Article

References (41)

1. Arivazhagan S, Balasenthil S, Nagini S (2000). Garlic and neem leaf extracts enhance hepatic glutathione and glutathione dependent enzymes during N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced gastric carcinogenesis in rats. Phytother. Res., 14: 291-293.
2. Atessahin A, Yilmaz S, Karahan I, Ceribasi AO, Karaoglu A (2005). Effects of lycopene against cisplatin-induced nephrotoxicity and oxidative stress in rats. Toxicol., 212: 116-123.
3. Babu E, Gopalakrishnan VK, Sriganth IN, Gopalakrishnan R, Sakthisekaran D (1995): Cisplatin induced nephrotoxicity and the modulating effect of glutathione ester. Mol. Cell. Biochem., 144: 7-11.
4. Bandyopadhyay U, Biswas K, Chatterjee R, Bandyopadhyay D, Chattopadhyay I, Ganguly CK, Chakraborty T, Bhattacharya K, Banerjee RK (2002). Gastroprotective effect of Neem (Azadirachta indica) bark extract: possible involvement of H(+)-K(+)-ATPase inhibition and scavenging of hydroxyl radical. Life Sci., 71: 2845-2865.
5. Belfield A, Goldberg DM (1971). Revised assay for serum phenyl phosphatase activity using 4-amino-antipyrine. Enzyme, 12: 561-573.
6. Bhanwra S, Singh J, Khosla P (2000). Effect of Azadirachta indica (Neem) leaf aqueous extract on paracetamol-induced liver damage in rats. Indian J. Physiol. Pharmacol., 44: 64-68.
7. Boeke SJ, Boersma MG, Alink GM, van Loon JJ, van Huis A, Dicke M, Rietjens IM (2004). Safety evaluation of neem (Azadirachta indica) derived pesticides. J. Ethnopharmacol., 94: 25-41.
8. Borch RF, Markman M (1989). Biochemical modulation of cisplatin toxicity. Pharmacol Ther., 41: 371-380.
9. Chirino YI, Pedraza-Chaverri J (2009): Role of oxidative and nitrosative stress in cisplatin-induced nephrotoxicity. Exp. Toxicol. Pathol., 61: 223-242.
10. El-Sayyad HI, Ismail MF, Shalaby FM, Abou-El-Magd RF, Gaur RL, Fernando A, Raj MH, Ouhtit A (2009): Histopathological effects of cisplatin, doxorubicin and 5-flurouracil (5-FU) on the liver of male albino rats. Int. J. Biol. Sci., 5: 466-473.
11. Farber E (1994). Programmed cell death: necrosis versus apoptosis. Mod. Pathol., 7: 605-609.
12. Hanigan MH, Devarajan P (2003). Cisplatin nephrotoxicity: molecular mechanisms. Cancer Ther., 1: 47-61.
13. Haque E, Mandal I, Pal S, Baral R (2006). Prophylactic dose of neem (Azadirachta indica) leaf preparation restricting murine tumor growth is nontoxic, hematostimulatory and immunostimulatory. Immunopharmacol. Immunotoxicol., 28: 33-50.
14. Husain K, Morris C, Whitworth C, Trammell GL, Rybak LP, Somani SM (1998). Protection by ebselen against cisplatin-induced nephrotoxicity: Antioxidant system. Mol. Cell. Biochem., 178: 127-133.
15. Kart A, Cigremis Y, Karaman M, Ozen H (2010). Caffeic acid phenethyl ester (CAPE) ameliorates cisplatin-induced hepatotoxicity in rabbit. Exp. Toxicol. Pathol., 62: 45-52.
16. Khan N, Mukhtar H (2008): Multitargeted therapy of cancer by green tea polyphenols. Cancer Lett., 269: 269-280.
17. Kim SH, Hong KO, Chung WY, Hwang JK, Park KK (2004). Abrogation of cisplatin-induced hepatotoxicity in mice by xanthorrhizol is related to its effect on the regulation of gene transcription. Toxicol. Appl. Pharmacol., 196: 346-355.
18. Kim YH, Kim YW, Oh YJ, Back NI, Chung SA, Chung HG, Jeong TS, Choi MS, Lee KT (2006). Protective effect of the ethanol extract of the roots of Brassica rapa on cisplatin-induced nephrotoxicity in LLCEzz-Din et al. 5505 PK1 cells and rats. Biol. Pharm. Bull., 29: 2436-2441.
19. Kintzel PE (2001). Anticancer drug-induced kidney disorders. Drug Saf., 24: 19-38.
20. Kishimoto S, Miyazawa K, Terakawa Y, Ashikari H, Ohtani A, Fukushima S, Takeuchi Y (2000). Cytotoxicity of cis-[((1R,2R)-1,2-cyclohexanediamine-N,N')bis(myristato)]-platinum (II) suspended in Lipiodol in a newly established cisplatin-resistant rat hepatoma cell line. Jpn. J. Cancer Res., 91: 1326-1332.
21. Lajolo PP, de Camargo B, del Giglio A (2009). Omission of day 2 of antiemetic medications is a cost saving strategy for improving chemotherapy-induced nausea and vomiting control: results of a randomized phase III trial. Am. J. Clin. Oncol., 32: 23-26.
22. Lee CK, Park KK, Hwang JK, Lee SK, Chung WY (2008). The extract of Prunus persica flesh (PPFE) attenuates chemotherapy-induced hepatotoxicity in mice. Phytother. Res., 22: 223-227.
23. Liao Y, Lu X, Lu C, Li G, Jin Y, Tang H (2008). Selection of agents for prevention of cisplatin-induced hepatotoxicity. Pharmacol. Res., 57: 125-131.
24. Maliakel DM, Kagiya TV, Nair CK (2008). Prevention of cisplatininduced nephrotoxicity by glucosides of ascorbic acid and alphatocopherol. Exp. Toxicol. Pathol., 60: 521-527.
25. Manikandan P, Letchoumy PV, Gopalakrishnan M, Nagini S (2008). Evaluation of Azadirachta indica leaf fractions for in vitro antioxidant potential and in vivo modulation of biomarkers of chemoprevention in the hamster buccal pouch carcinogenesis model. Food Chem. Toxicol., 46: 2332-2343.
26. Martins NM, Santos NA, Curti C, Bianchi ML, Santos AC (2008). Cisplatin induces mitochondrial oxidative stress with resultant energetic metabolism impairment, membrane rigidification and apoptosis in rat liver. J. Appl. Toxicol., 28: 337-344.
27. Moriya A, Hyodo I, Nishina T, Imaoka H, Imagawa A, Doi T, Endo H, Tanimizu M, Tajiri H (2000). Extensive liver metastasis of gastric cancer effectively treated by hepatic arterial infusion of 5-fluorouracil/cisplatin. Gastric Cancer, 3: 110-115.
28. Naziroglu M, Karaoglu A, Aksoy AO (2004). Selenium and high dose vitamin E administration protects cisplatin-induced oxidative damage to renal, liver and lens tissues in rats. Toxicol., 195: 221-230.
29. Peer PA, Trivedi PC, Nigade PB, Ghaisas MM, Deshpande AD (2008). Cardioprotective effect of Azadirachta indica A. Juss. on isoprenaline induced myocardial infarction in rats. Int. J. Cardiol., 126: 123-126.
30. Pratibha R, Sameer R, Rataboli PV, Bhiwgade DA, Dhume CY (2006). Enzymatic studies of cisplatin induced oxidative stress in hepatic tissue of rats. Eur. J. Pharmacol., 532: 290-293.
31. Rabik CA, Dolan ME (2007). Molecular mechanisms of resistance and toxicity associated with platinating agents. Cancer Treat. Rev., 33: 9-23.
32. Reitman S, Frankel S (1957). A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol., 28: 56-63.
33. Sadzuka Y, Shoji T, Takino Y (1992). Effect of cisplatin on the activities of enzymes which protect against lipid peroxidation. Biochem. Pharmacol., 43: 1872-1875.
34. Sarkar K, Bose A, Laskar S, Choudhuri SK, Dey S, Roychowdhury PK, Baral R (2007). Antibody response against neem leaf preparation recognizes carcinoembryonic antigen. Int. Immunopharmacol., 7: 306-312.
35. Schmidt M, Eisenburg J (1975). Serum bilirubin determination in newborn infants. A new micromethod for the determination of serum of plasma bilirubin in newborn infants. Fortschr. Med., 93: 1461-1466.
36. Szasz G (1969). A kinetic photometric method for serum gammaglutamyl transpeptidase. Clin. Chem., 15: 124-136.
37. Vickers AE, Rose K, Fisher R, Saulnier M, Sahota P, Bentley P (2004). Kidney slices of human and rat to characterize cisplatin-induced injury on cellular pathways and morphology. Toxicol. Pathol., 32: 577-590.
38. Vinothini G, Manikandan P, Anandan R, Nagini S (2009). Chemoprevention of rat mammary carcinogenesis by Azadirachta indica leaf fractions: modulation of hormone status, xenobioticmetabolizing enzymes, oxidative stress, cell proliferation and apoptosis. Food Chem. Toxicol., 47: 1852-1863.
39. Yanpallewar SU, Sen S, Tapas S, Kumar M, Raju SS, Acharya SB (2003). Effect of Azadirachta indica on paracetamol-induced hepatic damage in albino rats. Phytomedicine, 10: 391-396.
40. Yao X, Panichpisal K, Kurtzman N, Nugent K (2007). Cisplatin nephrotoxicity: a review. Am. J. Med. Sci., 334: 115-124.
41. Zhang JG, Lindup WE (1993). Role of mitochondria in cisplatin-induced oxidative damage exhibited by rat renal cortical slices. Biochem. Pharmacol., 45: 2215-2222.