Depletion of highly abundant proteins in blood plasma by ammonium sulfate precipitation for 2D-PAGE analysis

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 879, Issue 30, 2011, Pages 3645-3648

  Mahn, Andrea ^a   Ismail, Maritza ^a  

^a UNIVERSIDAD DE SANTIAGO DE CHILE   (Chile)

Author keywords

Ammonium sulfate precipitation; Plasma proteomics; Protein depletion; Two dimensional gel electrophoresis

Indexed keywords

2D-PAGE; AMMONIUM SULFATE; AMMONIUM SULFATE PRECIPITATION; ANTITRYPSIN; BLOOD PLASMA; DYNAMIC RANGE; HIGH REPRODUCIBILITY; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; LOW-ABUNDANCE PROTEINS; PLASMA PROTEIN; PROTEIN CONCENTRATIONS; PROTEIN DEPLETION; PROTEOMIC ANALYSIS; RELATIVE ABUNDANCE; TWO-DIMENSIONAL GEL ELECTROPHORESIS;

AMMONIUM COMPOUNDS; BLOOD; ELECTROPHORESIS; GELS; HYDROPHOBIC CHROMATOGRAPHY; HYDROPHOBICITY; LIQUID CHROMATOGRAPHY; MOLECULAR BIOLOGY; PLASMAS;

PROTEINS;

ALBUMIN; ALPHA 1 ANTITRYPSIN; AMMONIUM SULFATE;

ANIMAL EXPERIMENT; ARTICLE; CONCENTRATION (PARAMETERS); HYDROPHOBIC INTERACTION CHROMATOGRAPHY; IMMUNOAFFINITY CHROMATOGRAPHY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEOMICS; RAT; TWO DIMENSIONAL ELECTROPHORESIS;

ALBUMINS; ALPHA 1-ANTITRYPSIN; AMMONIUM SULFATE; ANIMALS; BLOOD PROTEINS; CHEMICAL PRECIPITATION; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; IMMUNOSORBENT TECHNIQUES; PROTEOMICS; RATS; RATS, WISTAR; REPRODUCIBILITY OF RESULTS;

EID: 80555150623     PISSN: 15700232     EISSN: 1873376X     Source Type: Journal    
DOI: 10.1016/j.jchromb.2011.09.024     Document Type: Article

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