The cytotoxicity of methacryloxylethyl cetyl ammonium chloride, a cationic antibacterial monomer, is related to oxidative stress and the intrinsic mitochondrial apoptotic pathway

Brazilian Journal of Medical and Biological Research

Volumn 44, Issue 11, 2011, Pages 1125-1133

  Sai, Ma ^a   Le Qun, Shan ^a   Yu Hong, Xiao ^b   Fang, Li ^a   Li, Huang ^a   Shen, Lijuan ^a   Ji Hua, Chen ^a  

^a FOURTH MILITARY MEDICAL UNIVERSITY   (China)

^b KUNMING GENERAL HOSPITAL   (China)

Author keywords

Apoptosis; Cytotoxicity; Dental monomer; Methacryloxylethyl cetyl ammonium chloride; Quaternary ammonium compounds; Reactive oxygen species

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; AMMONIUM CHLORIDE; BIOMATERIAL; CYTOCHROME C; DICHLORODIHYDROFLUORESCEIN DIACETATE; METHACRYLOXYLETHYL CETYL AMMONIUM CHLORIDE; MONOMER; PROTEIN BAX; PROTEIN BCL 2; REACTIVE OXYGEN METABOLITE; UNCLASSIFIED DRUG;

ANIMAL CELL; ANTIBACTERIAL ACTIVITY; APOPTOSIS; ARTICLE; CELL ASSAY; CELL CYCLE ARREST; CELL CYCLE G1 PHASE; CELL CYCLE G2 PHASE; CELL CYCLE PROGRESSION; CELL LEVEL; CELL STRAIN L 929; CONTROLLED STUDY; CYTOTOXICITY; FLOW CYTOMETRY; MITOCHONDRIAL MEMBRANE POTENTIAL; MITOCHONDRION; MOUSE; NONHUMAN; OXIDATIVE STRESS; PROTEIN EXPRESSION; PROTEIN SECRETION; STAINING; WESTERN BLOTTING;

EID: 80455162591     PISSN: 0100879X     EISSN: 1414431X     Source Type: Journal    
DOI: 10.1590/S0100-879X2011007500130     Document Type: Article

References (28)

1. De Munck J, Van Landuyt K, Peumans M, Poitevin A, Lambrechts P, Braem M, et al. A critical review of the durability of adhesion to tooth tissue: methods and results. J Dent Res 2005; 84: 118-132.
2. Imazato S. Bio-active restorative materials with antibacterial effects: new dimension of innovation in restorative dentistry. Dent Mater J 2009; 28: 11-19.
3. Li F, Chai ZG, Sun MN, Wang F, Ma S, Zhang L, et al. Antibiofilm effect of dental adhesive with cationic monomer. J Dent Res 2009; 88: 372-376.
4. Xiao YH, Ma S, Chen JH, Chai ZG, Li F, Wang YJ. Antibacterial activity and bonding ability of an adhesive incorporating an antibacterial monomer DMAE-CB. J Biomed Mater Res B Appl Biomater 2009; 90: 813-817.
5. Li F, Chen J, Chai Z, Zhang L, Xiao Y, Fang M, et al. Effects of a dental adhesive incorporating antibacterial monomer on the growth, adherence and membrane integrity of Streptococcus mutans. J Dent 2009; 37: 289-296.
6. Schweikl H, Spagnuolo G, Schmalz G. Genetic and cellular toxicology of dental resin monomers. J Dent Res 2006; 85: 870-877.
7. Hume WR, Gerzina TM. Bioavailability of components of resin-based materials which are applied to teeth. Crit Rev Oral Biol Med 1996; 7: 172-179.
8. Noda M, Wataha JC, Kaga M, Lockwood PE, Volkmann KR, Sano H. Components of dentinal adhesives modulate heat shock protein 72 expression in heat-stressed THP-1 human monocytes at sublethal concentrations. J Dent Res 2002; 81: 265-269.
9. Nishida M, Imazato S, Takahashi Y, Ebisu S, Ishimoto T, Nakano T, et al. The influence of the antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide on the proliferation, differentiation and mineralization of odontoblast-like cells. Biomaterials 2010; 31: 1518-1532.
10. Gupta S, Kass GE, Szegezdi E, Joseph B. The mitochondrial death pathway: a promising therapeutic target in diseases. J Cell Mol Med 2009; 13: 1004-1033.
11. Kroemer G. The proto-oncogene Bcl-2 and its role in regulating apoptosis. Nat Med 1997; 3: 614-620.
12. Ratanasathien S, Wataha JC, Hanks CT, Dennison JB. Cytotoxic interactive effects of dentin bonding components on mouse fibroblasts. J Dent Res 1995; 74: 1602-1606.
13. Sturrock JE, Nunn JF. Chromosomal damage and mutations after exposure of Chinese hamster cells to high concentrations of oxygen. Mutat Res 1978; 57: 27-33.
14. Chang MC, Lin LD, Chan CP, Chang HH, Chen LI, Lin HJ, et al. The effect of BisGMA on cyclooxygenase-2 expression, PGE2 production and cytotoxicity via reactive oxygen species- and MEK/ERK-dependent and -independent pathways. Biomaterials 2009; 30: 4070-4077.
15. Chang HH, Guo MK, Kasten FH, Chang MC, Huang GF, Wang YL, et al. Stimulation of glutathione depletion, ROS production and cell cycle arrest of dental pulp cells and gingival epithelial cells by HEMA. Biomaterials 2005; 26: 745-753.
16. Stanislawski L, Lefeuvre M, Bourd K, Soheili-Majd E, Goldberg M, Perianin A. TEGDMA-induced toxicity in human fibroblasts is associated with early and drastic glutathione depletion with subsequent production of oxygen reactive species. J Biomed Mater Res A 2003; 66: 476-482.
17. Chang HH, Chang MC, Lin LD, Lee JJ, Wang TM, Huang CH, et al. The mechanisms of cytotoxicity of urethane di methacrylate to Chinese hamster ovary cells. Biomaterials 2010; 31: 6917-6925.
18. Shackelford RE, Kaufmann WK, Paules RS. Oxidative stress and cell cycle checkpoint function. Free Radic Biol Med 2000; 28: 1387-1404.
19. Shackelford RE, Innes CL, Sieber SO, Heinloth AN, Leadon SA, Paules RS. The Ataxia telangiectasia gene product is required for oxidative stress-induced G1 and G2 checkpoint function in human fibroblasts. J Biol Chem 2001; 276: 21951-21959.
20. Boonstra J, Post JA. Molecular events associated with reactive oxygen species and cell cycle progression in mammalian cells. Gene 2004; 337: 1-13.
21. Ryter SW, Kim HP, Hoetzel A, Park JW, Nakahira K, Wang X, et al. Mechanisms of cell death in oxidative stress. Antioxid Redox Signal 2007; 9: 49-89.
22. Paranjpe A, Bordador LC, Wang MY, Hume WR, Jewett A. Resin monomer 2-hydroxyethyl methacrylate (HEMA) is a potent inducer of apoptotic cell death in human and mouse cells. J Dent Res 2005; 84: 172-177.
23. Janke V, von Neuhoff N, Schlegelberger B, Leyhausen G, Geurtsen W. TEGDMA causes apoptosis in primary human gingival fibroblasts. J Dent Res 2003; 82: 814-818.
24. Engelmann J, Janke V, Volk J, Leyhausen G, von Neuhoff N, Schlegelberger B, et al. Effects of BisGMA on glutathione metabolism and apoptosis in human gingival fibroblasts in vitro. Biomaterials 2004; 25: 4573-4580.
25. Marcotte L, Barbeau J, Lafleur M. Permeability and thermodynamics study of quaternary ammonium surfactantsphosphocholine vesicle system. J Colloid Interface Sci 2005; 292: 219-227.
26. Ott M, Gogvadze V, Orrenius S, Zhivotovsky B. Mitochondria, oxidative stress and cell death. Apoptosis 2007; 12: 913-922.
27. Lefeuvre M, Amjaad W, Goldberg M, Stanislawski L. TEGDMA induces mitochondrial damage and oxidative stress in human gingival fibroblasts. Biomaterials 2005; 26: 5130-5137.
28. Kelekar A, Thompson CB. Bcl-2-family proteins: the role of the BH3 domain in apoptosis. Trends Cell Biol 1998; 8: 324-330.