DNA extraction from paraffin embedded material for genetic and epigenetic analyses

Journal of Visualized Experiments

Volumn , Issue 49, 2011, Pages

  Pikor, Larissa A ^a,b   Enfield, Katey S S ^a,b   Cameron, Heryet ^c   Lam, Wan L ^a,b  

^a BRITISH COLUMBIA CANCER RESEARCH CENTRE   (Canada)

^b UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^c BRITISH COLUMBIA CANCER AGENCY   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80355149133     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2763     Document Type: Article

References (7)

1. Santos, M.C., Saito, C.P. & Line, S.R. Extraction of genomic DNA from paraffin-embedded tissue sections of human fetuses fixed and stored in formalin for long periods. Pathol Res Pract 204, 633-6 (2008).
2. Hilz, H., Wiegers, U. & Adamietz, P. Stimulation of proteinase K action by denaturing agents: application to the isolation of nucleic acids and the degradation of 'masked' proteins. Eur J Biochem 56, 103-8 (1975).
3. Joseph Sambrook, D.R. Molecular Cloning: A Laboratory Manual, (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 2001).
4. Kennett, J.Y., Watson, S.K., Saprunoff, H., Heryet, C. & Lam, W.L. Technical demonstration of whole genome array comparative genomic hybridization. J Vis Exp (2008).
5. Thu, K.L. et al. Methylated DNA immunoprecipitation. J Vis Exp (2009).
6. Tang, W. et al. DNA extraction from formalin-fixed, paraffin-embedded tissue. Cold Spring Harb Protoc 2009, pdb prot5138 (2009).
7. Hongxin Fan, M.L.G. DNA Extraction from Paraffin-Embedded Tissues. Molecular Pathology Protocols 49, 1-4 (2001).